检索结果-南通市图书馆

nuclear factor kappa b: A marker of chemotherapy for human stage Ⅳ gastric carcinoma

World Journal of Gastroenterology 2008年第30期14卷 4739-4744页

作者： Sheng Ye You-Ming Long Jian Rong Wen-Rui Xie Oncology Department the First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China Clinical College Guangdong Pharmaceutical University Guangzhou 510080 Guangdong Province China Jian Rong Department of Anesthesiology the First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510080 Guangdong Province China the First Affiliated Hospital of Guangdong Pharmaceutical University Guangzhou 510080 Guangdong Province. China

AIM： To detect the nuclear factor kappa b （NF-κb） condition in human stage IV gastric carcinoma patients and to explore the correlation between NF-κb activation and survival of these patients after chemotherapy. METHODS： Expression of NF-κb-p65 was determined by immunohistochemical analysis. Activity of NF-κb DNA-binding in carcinoma tissue was detected by electrophoretic mobility shift assay. Kaplan-Meier survival analysis was performed to show the relation between NF-κb and progression-free survival （PFS） or overall survival （OS） of the patients. RESULTS： The positive expression rate of NF-κb-p65 in 60 gastric cancer tissue samples was 76.7% （46160）. The expression of NF-κb-p65 was reduced in adjacent carcinoma and normal tissue samples. Electrophoretic mobility shift assay （EMSA） analysis showed a strong activation of NF-κb in cancer tissue samples. A survival difference was found in NF-κb-p65 positive and negative patients. NF-κb-p65 expression was negative in cancer tissue samples （n = 14）. PFS was 191.40 ± 59.88 d and 152.93 ±16.99 d, respectively, in patients with positive NF-κb-p65 expression （n = 46）（P = 0.4028）. The survival time of patients with negative and positive NF-κb-p65 expression was 425.16 ±61.61 d and 418.85 ±42.98 d, respectively （P = 0.7303）. Kaplan-Meier analysis showed no significant difference in PFS or OS. The 46 patient tissue which positive NF-κb-p65 expression was found in the tissue samples from the 46 patients whose PFS and OS were 564.89 ± 75.94 d and s 352.37 ±41.32 d, respectively （P = 0.0165）. CONCLUSION： NF-κb is activated in gastric carcinoma tissue, which is related to the OS after chemotherapy.

关键词： Gastric carcinoma nuclear factor kappa b Activation Survival analysis Therapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Interleukin-10 inhibits the expression of nuclear factor kappa b in rats with cerebral ischemia

引用

Neural Regeneration Research 2006年第9期1卷 781-784页

作者： Zhihua Wu Nan Liu Houwei Du Ronghua Chen An Zheng Huapin Huang Department of Neurology the Affiliated Union Hospital of Fujian Medical UniversityFuzhou 350001Fujian ProvinceChina

bACKGROUND:Plenty of studies have demonstrated that inflammatory reaction is involved in ischemic cerebral damage,and the expression of inflammatory cytokines can be observed at the initial sites of cerebral damage at early period,including interleukin-6,interleukin-8,etc.,which are all the target gene products of nuclear factor kappa b(NF-κb).The process of ischemic damage can be affected by adjusting and controlling NF-κb activity via ***:To investigate the inhibitory effect of interleukin-10 on the expression of NF-κb in the ischemic sites of rats with focal cerebral ischemia in rats and its molecular ***:A randomized and controlled animal ***:Department of Neurology,the Affiliated Union Hospital of Fujian Medical ***:Thirty-two adult male Sprague-Dawley rats weighing(250±30)g were ***-κb p65(RelA)rabbit anti-rat monoclonal primary antibody was the product of Neomarkers Company;Immunohistochemical kit of the SP two-step method was purchased from beijing Zhongshan biotechnology Co.,***:The experiment was carried out in the Affiliated Union Hospital of Fujian Medical University from August 2005 to April *** rats were randomly assigned into sham-operated group,middle cerebral artery occlusion(MCAO)group,vehicle-treated group and interleukin-10 treated group,8 rats in each *** cerebral ischemia was induced by occlusion of the middle cerebral artery as previously *** in the MCAO group were anesthetized intraperitoneally,thyroid was bluntly *** common,external and internal carotid arteries were isolated,the trunk of external carotid artery was ligated and freed,an artery clamp was placed at the internal carotid artery,then a'V'shape incision was made at the free section of external carotid artery,filament was inserted for a depth of(18.5±0.5)*** rats in the sham-operated group were given the same treatments with the exception of filament *** the suc

关键词： focal cerebral ischemia middle cerebral artery internal carotid artery lateral ventricle nuclear factor kappa b neurological deficits inflammatory reaction molecular mechanisms rectal temperature inhibitory effect cascade reaction carotid arteries target gene

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recombinant osteopontin attenuates hyperoxia-induced acute lung injury through inhibiting nuclear factor kappa b and matrix metalloproteinases 2 and 9

引用

Chinese Medical Journal 2014年第23期127卷 4025-4030页

作者： Zhang Xiangfeng Liu Fen Zhu Guangfa Wang Zengzhi Department of Respiratory Medicine Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Epidemiology and Statistics School of Public Health Capital Medical University Beijing 100069 China

background Exposure of adult mice to more than 95％ O2 produces a lethal injury by 72 *** factor kappa b （NF-κb） is a transcriptional factor that plays a key role in the modulation of cytokine networks during hyperoxia-induced acute lung injury （ALl）.Osteopontin （OPN） is a phosphorylated glycoprotein produced principally by *** have reported that exogenous OPN can maintain the integrity of the cerebral microvascular basement membrane and reduce brain damage through inhibiting NF-κb activities in the brain after subarachnoid ***,it is not clear whether OPN can reduce lung injury during ALl by inhibiting transcriptional signal pathways of NF-κb and consequent inhibition of infiammatory *** we examined the effects and mechanisms of recombinant OPN （r-OPN） on *** Ninety-six mice were randomly divided into phosphate buffered saline （PbS） and r-OPN *** were put in an oxygen chamber （＞95％ O2） and assessed for lung injury at 24,48,and 72 *** of NF-κb,matrix metalloproteinases 2 and 9 （MMP-2 and MMP-9）,and tissue inhibitors of MMP-2 and MMP-9 （TIMP-1,TIMP-2） mRNA in lungs were examined with *** and distribution of NF-κb protein in lungs were measured with *** Exposure to hyperoxia for 72 hours induced more severe lung injury in the PbS group compared with the r-OPN *** of NF-κb mRNA in the PbS group exposed to hyperoxia for 48 and 72 hours was significantly higher than the r-OPN group （P ＜0.05）.With 72-hour exposure,expression of TIMP-1 mRNA in the r-OPN group was significantly higher than that of the PbS group （P ＜0.05）.Expression of TIMP-2 mRNA in the r-OPN group at 48 and 72 hours was significantly higher than those in the PbS group （P ＜0.05）.After 72-hour exposure,expression of NF-κb protein in airway epithelium in the PbS group was significantly higher than that in the r-OPN group （P ＜0.05）.Conclusion r-OPN can inhibit the release and acti

关键词： osteopontin hyperoxia acute lung injury nuclear factor kappa b matrix metalloproteinase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Tumor metastasis and the reciprocal regulation of heparanase gene expression by nuclear factor kappa b in human gastric carcinoma tissue

引用

World Journal of Gastroenterology 2005年第6期11卷 903-907页

作者： Hou-JunCao YongFang XingZhang Wen-JunChen Wen-PengZhou HongWang Lin-boWang Jin-MinWu CenterofOncology SirRunRunShawHospitalZhejiangUniversitySchoolofMedicineHangzhou310016ZhejiangProvinceChina

AIM: To investigate whether NF-kb is activated in human gastric carcinoma tissues and, if so, to study whether there is any correlation between NF-kb activity and heparanase expression in gastric carcinoma. METHODS: NF-kb activation was assayed by immunohistochemical staining in formalin-fixed, paraffin-embedded specimens from 45 gastric carcinoma patients. Electrophoretic mobility shift assay (EMSA) method was used for nuclear protein from these fresh tissue specimens. Heparanase gene expression was quantified using quantitative RT-PCR. RESULTS: The nuclear translocation of RelA (marker of NF-kb activation) was significantly higher in tumor cells compared to adjacent and normal epithelial cells [(41.3±3.52)% vs (0.38±0.22) %, t=10.993, P= 0.000b activation was correlated with tumor invasion-related clinicopathological features such as lymphatic invasion, pathological stage, and depth of invasion (Z= 2.148, P= 0.032b activation was significantly correlated with expression of heparanase gene (r= 0.194, P=0.046b RelA (p65) activation was related with increased heparanase gene expression and correlated with poor clinicopathological characteristics in gastric cancers. This suggests NF-kb as a major controller of the metastatic phenotype through its reciprocal regulation of some metastasis-related genes.

关键词： nuclear factor kappa b Heparanase gene Metastasis Gastric carcinoma

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Inhibition of p38 mitogen-activated protein kinase attenuates experimental autoimmune hepatitis: Involvement of nuclear factor kappa b

引用

World Journal of Gastroenterology 2007年第31期13卷 4249-4254页

作者： Xiong Ma Yi-Tao Jia De-Kai Qiu ShanghaiJiaotong University School of Medicine Shanghai Institute ofDigestive Disease Shanghai 200001 China

To investigate the role of p38 mitogen-activated protein kinase （p38MAPK） in murine experimental autoimmune hepatitis （EAH）.METHODS： To induce EAH, the syngeneic S-100 antigen emulsified in complete Freud＇s adjuvant was injected intraperitoneally into adult male C57bI/6 mice. Liver injury was assessed by serum ALT and liver histology. The expression and activity of p38 MAPK were measured by Western blot and kinase activity assays. In addition, DNA binding activities of nuclear factor kappa b （NF-Kb） were analyzed by electrophoretic mobility shift assay. The effects of Sb203580, a specific p38 MAPK inhibitor, on liver injuries and expression of proinflammatory cytokines （interferon-y, IL-12, IL-1β and TNF-α） were ***： The activity of p38 MAPK and NF-~：b was increased and reached its peak 14 or 21 d after the first syngeneic S-100 administration. Inhibition of p38 MAPK activation by Sb203580 decreased the activation of NF-~：b and the expression of proinflammatory cytokines. Moreover, hepatic injuries were improved significantly after Sb203580 administration.

关键词： Autoimmune hepatitis p38 mitogen-activatedprotein kinase nuclear factor kappa b Proinflammatorycytokines

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Regulatory Effects of Polyporus Polysaccharide on the nuclear factor kappa b Signal Pathway of bladder Cancer Cells Stimulated by bacillus Calmette-Guerin

引用

Chinese Journal of Integrative Medicine 2011年第7期17卷 531-536页

作者：危建安曾星韩凌黄羽 The Central Laboratory the Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine

Objective：To detect the effects of Polyporus polysaccharide（PPS）,bacillus Calmette-Guerin （bCG）,and their combination on the nuclear factor kappa b（NF-κb）signaling pathway associated-gene expression and investigate the molecular mechanisms of the toxic-reducing effect of PPS in coordination with bCG against bladder ***：After T739 cells were treated with PPS,bCG and their combination, the changes in mRNA and protein expression of inhibitor of kappa b kinase beta（IKKβ）,NF-κb subunit p65 （NF-κb p65）,intracellular adhesion molecule 1（ICAM1）and chemokine（C-c motif）ligand 2（CCL2）in bladder cancer cell line T739 were determined by relative quantitative real-time PCR,Western blot,and flow cytometry （FCM）.NF-κb p65 DNA-binding activity in T739 cell was detected by biotinylated probe-ELISA,and NF-κb p65 nuclear expression in T739 cell was observed by ***：Compared with the T739 control group,the mRNA expression of IKbKb（IKKβ）,Rel A（NF-κb p65）,ICAM1 and CCL2 in T739 cells treated with bCG were increased obviously（Ratio2.0）,as well as the expression of IKKβ,CCL2 and ICAM1 ***,NF-κb p65 DNA-binding activity and NF-κb p65 nuclear expression in T739 cells treated with bCG were up-regulated significantly（P0.05）.Compared with the control,the increased expression in T739 cells were simultaneously down-regulated after PPS treatment,except for ICAM1 protein *** cells treated with a combination of bCG and PPS,the expression of genes associated with the NF-κb signaling pathway,such as IKbKb,ICAM1 and CCL2,were all down-regulated compared to the bCG group,as well as Rel A mRNA expression,NF-κb p65 DNA-binding activity and NF-κb p65 nuclear ***： PPS could inhibit the over-activation of the NF-κb signaling pathway induced by bCG in bladder cancer cells and accordingly attenuate the adverse reactions to bCG therapy.

关键词： Polyporus polysaccharide bacillus Calmette-Guerin bladder cancer nuclear factor kappa b signaling pathway

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of the regimen of Gaoshan Hongjingtian on the mechanism of poly（ADP-ribose） polymerase regulation of nuclear factor kappa b in the experimental diabetic retinopathy

引用

Chinese Medical Journal 2013年第9期126卷 1693-1699页

作者： ZHAO Hong-shu SHI Xiang-yu WEI Wen-bin WANG Ning-li beijing Tongren Eye Center Beijing Tongren Hospital Capital Medical University beijing Ophthalmology & Visual Sciences Key Laboratory Beijing 100730 China

background Poly（ADP-ribose） polymerase （PARP） plays an important role in the death of retinal capillary cells in diabetic retinopathy （DR） partly via its regulation of nuclear factor kappa b （NF-κb）. The current study investigated the effect of the regimen of Gaoshan Hongjingtian （RG） on the mechanism of PARP regulation of NF-κb, and demonstrated the possible impact of the RG and Gaoshan Hongjingtian （Rhodiola sachalinensis, RS） on diabetic retinopathy. Methods Wistar rats were made diabetic by administering streptozotocin. They were then assigned to three groups at random. After 2 months, the three groups of these diabetic rats were treated with RS or RG, or untreated. Analyses of expression levels of PARP, NF-κb, and intercellular adhesion molecule-1 （ICAM-1） in the retinas of rats in different groups were performed by Western blotting and immunohistochemical assays, and mRNA levels of NF-κb and ICAM-1 were determined by real-time polymerase chain reaction （PCR）. In addition, the basement membranes of capillaries in the rats＇ retinas were observed using electron microscopy, and diabetes-induced capillary degeneration （ghost pericytes and acellular capillaries） were quantitated. Results From the third month after the injection of streptozotocin, the diabetic rats were given daily RG, RS or tap water separately. The diabetic rats failed to gain weight compared with normal age-matched rats, whereas their glycated hemoglobin levels were significantly increased. After 5 months, the mRNA levels of NF-Kb and ICAM-1 and the protein expression of PARP, NF-κb, and ICAM-1 were significantly increased in the retinas of diabetic rats in the untreated group compared with the nondiabetic controls. After 8 months, the number of degenerated retinal capillaries （ghost pericytes and acellular capillaries） was significantly increased in the diabetic rats in the untreated group compared with normal age-matched rats. RG and RS inhibited diabetes-induced over-expression of PARP, NF-Kb,

关键词： Rhodiola sachalinensis Gaoshan Hongjingtian diabetic retinopathy poly(ADP-ribose) polymerase nuclear factor kappa b

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

bMS-345541 inhibited nuclear factor kappa b expression and improved locomotor function recovery in rats after acute spinal cord injury

引用

Neural Regeneration Research 2011年第23期6卷 1775-1779页

作者： Xin Han Shouyu Wang Zhen Zhang Decheng Lu Hairun Liu Department of Orthopedic Surgery First Affiliated Hospital of Dalian Medical University Dalian 116000 Liaoning Province China

This study sought to elucidate the changes of nuclear factor kappa b （NF-Kb） expression and locomotor function of hind limb after subdural injection of bMS-345541 was applied in rats with acute spinal cord injury. The results indicated that bMS-345541 treatment reduced the expression of NF-kb at 24 hours after injury, compared with normal saline-treated rats. This treatment also led to a significant improvement in locomotor functional recovery at 14 days after injury. Overall, the findings demonstrated that bMS-345541 significantly ameliorated spinal cord injury-induced hind limb dysfunction by inhibiting the expression of NF-kb after spinal cord injury.

关键词： spinal cord injury bMS-345541 nuclear factor kappa b locomotor function neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Up-regulation of intestinal nuclear factor kappa b and intercellular adhesion molecule-1 following traumatic brain injury in rats

引用

World Journal of Gastroenterology 2005年第8期11卷 1149-1154页

作者： Chun-HuaHang Ji-XinShi Jie-ShouLi Wei-QinLi Hong-XiaYin DepartmentofNeurosurgery JinlingHospitalClinicalSchoolofMedicineNanjingUniversityNanjing210002JiangsuProvinceChina ResearchInstituteofGeneralSurgery JinlingHospitalClinicalSchoolofMedicineNanjingUniversityNanjing210002JiangsuProvinceChina

AIM: nuclear factor kappa b (NF-κb) regulates a large number of genes involved in the inflammatory response to critical illnesses, but it is not known if and how NF-Kb is activated and intercellular adhesion molecule-1 (ICAM-1) expressed in the gut following traumatic brain injury (TbI). The aim of current study was to investigate the temporal pattern of intestinal NF-κb activation and ICAM-1 expression following TbI. METHODS: Male Wistar rats were randomly divided into six groups (6 rats in each group) including controls with sham operation and TbI groups at hours 3, 12, 24, and 72, and on d 7. Parietal brain contusion was adopted using weight-dropping method. All rats were decapitated at corresponding time point and mid-jejunum samples were taken. NF-Kb binding activity in jejunal tissue was measured using EMSA. Immunohistochemistry was used for detection of ICAM-1 expression in jejunal samples. RESULTS: There was a very low NF-κb binding activity and little ICAM-1 expression in the gut of control rats after sham surgery. NF-Kb binding activity in jejunum significantly increased by 160% at 3 h following TbI (Pby 390% increase. Compared to controls, ICAM-1 was significantly up-regulated on the endothelia of microvessels in villous interstitium and lamina propria by 24 h following TbI and maximally expressed at 72 h post-injury (Pb activation and endothelial ICAM-1 expression coincided in time with the period during which secondary mucosal injury of the gut was also at their culmination following TbI. CONCLUSION: TbI could induce an immediate and persistent up-regulation of NF-κb activity and subsequent up-regulation of ICAM-1 expression in the intestine. Inflammatory response mediated by increased NF-κb activation and ICAM-1 expression may play an impor

关键词： Traumatic brain injury Intestine nuclear factor kappa b Intercellular adhesion molecule-1 Inflammatory response

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Role of nuclear factor kappa b in central nervous system regeneration

引用

Neural Regeneration Research 2014年第7期9卷 707-711页

作者： Christian Engelmann Falk Weih Ronny Haenold Leibniz Institute for Age Research-Fritz Lipmann Institute

Activation of nuclear factor kappa b （NF-κb） is a hallmark of various central nervous system （CNS） pathologies. Neuron-specific inhibition of its transcriptional activator subunit RelA, also referred to as p65, promotes neuronal survival under a range of conditions, i.e., for ischemic or excitotoxic insults. In macro- and microglial cells, post-lesional activation of NF-κb triggers a growth-permissive program which contributes to neural tissue inflammation, scar formation, and the expression of axonal growth inhibitors. Intriguingly, inhibition of such inducible NF-~b in the neuro-glial compartment, i.e., by genetic ablation of RelA or overexpression of a trans- dominant negative mutant of its upstream regulator Iκba, significantly enhances functional recovery and promotes axonal regeneration in the mature CNS. by contrast, depletion of the NF-κb subunit p50, which lacks transcriptional activator function and acts as a transcriptional repressor on its own, causes precocious neuronal loss and exacerbates axonal degeneration in the lesioned brain. Collectively, the data imply that NF-κb orchestrates a multicellular pro- gram in which κb-dependent gene expression establishes a growth-repulsive terrain within the post-lesioned brain that limits structural regeneration of neuronal circuits. Considering these subunit-specific functions, interference with the NF-κb pathway might hold clinical potentials to improve functional restoration following traumatic CNS injury.

关键词： nuclear factor kappa b RelA p65 p50 central nervous system injury axonal regeneration neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：