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A taxonomic re-assessment in the Chinese Bupleurum(Apiaceae):Insights from morphology,nuclear ribosomal internal transcribed spacer,and chloroplast(trnH-psbA,matK) sequences

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Journal of Systematics and Evolution 2011年第6期49卷 558-589页

作者： Chang-Bao WANG Xiang-Guang MA Xing-Jin HE(College of Life Science,Sichuan University,Chengdu 610064,China) (College of Life Science Sichuan University Chengdu 610064 China)

As one of the big genera in Apiaceae,Bupleurum *** received considerable attention from plant ***,there are still many gaps in our knowledge of this genus,especially of those species endemic to China that have often been excluded from previous *** spite of a few recent studies on the phylogeny and classification of the Chinese Bupleurum,the most essential problem of species delimitation still remains *** re-evaluate the taxonomy of the Chinese Bupleurum using morphological and molecular *** observations of living plants growing the field and herbarium specimens were made to understand the morphological variation and to ensure species identification for the molecular *** analyses were carried out on sequence data of nuclear ribosomal internal transcribed spacer(nrits),trnH-psbA,and matK,using parsimony and Bayesian approaches to evaluate species *** of both nrits and combined datasets of the three regions revealed the following results:(i)the Chinese Bupleurum species were divided into two major lineages;and(ii)most species were well defined,but some traditional inter-and infraspecific relationships were not *** results,along with the results of previous works support that the Chinese Bupleurum species were derived from two different lineages and should be placed in Neves and Watson’s subgenus *** detailed taxonomic re-assessment of several Chinese species resulted in new taxonomic recombinations with rank changes for three species:Bupleurum tenerum,***,and ***;new combination for one variety,*** ***;and the description of one variety and one species,*** *** and ***.

关键词： Chinese Bupleurum chloroplast sequences(trnH-psbA matK) morphology nrits phylogenetic analyses taxonomy and new species.

A new orchid Paphiopedilum guangdongense and its molecular evidence

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Journal of Systematics and Evolution 2010年第5期48卷 350-355页

作者： 1 Li-Jun CHEN 1,2,3Zhong-Jian LIU 4You-Yu LI 1Li-Qiang LI 1(Shenzhen Key Laboratory for Orchid Conservation and Utilization,The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen,Shenzhen 518114,China) 2(The Center for Biotechnology and BioMedicine,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055,China) 3(College of Forestry,South China Agricultural University,Guangzhou 510642,China) 4(The Nature Reserve of Dawuling of Guangdong Province,Xinyi 525000,Guangdong,China) (Shenzhen Key Laboratory for Orchid Conservation and Utilization The National Orchid Conservation Center of China/The Orchid Conservation and Research Center of Shenzhen Shenzhen 518114 China) (The Center for Biotechnology and BioMedicine Graduate School at Shenzhen Tsinghua University Shenzhen 518055 China) (College of Forestry South China Agricultural University Guangzhou 510642 China) (The Nature Reserve of Dawuling of Guangdong Province Xinyi 525000 Guangdong China)

A new Paphiopedilum entity found in southern Guangdong was studied taxonomically by means of internal transcribed spacer of nuclear ribosomal DNA analysis in addition to morphological and phytogeographical *** entity is similar to Paphiopedilum gratrixianum(Masters)Rolfe morphologically,from which it differs by having narrower leaves not wider than 2 cm and lacking purple spots toward abaxial base,pale green dorsal sepal veined with pale purplish brown and not heavily spotted with purple,and staminode subtruncate and mucronate at *** analysis of internal transcribed spacer sequences of this entity and its allies came to the same conclusion that the entity is a member of Paphiopedilum section Paphiopedilum and closely related to but distinct from *** a result,it is treated here as a new species,Paphiopedilum guangdongense ***&***.

关键词： new species nrits Orchidaceae Paphiopedilum guangdongense.

Phylogenetic incongruence in Cymbidium orchids

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Plant Diversity 2021年第6期43卷 452-461页

作者： Guo-Qiang Zhang Gui-Zhen Chen Li-Jun Chen Jun-Wen Zhai Jie Huang Xin-Yi Wu Ming-He Li Dong-Hui Peng Wen-Hui Rao Zhong-Jian Liu Si-Ren Lan Forestry College of Fujian Agriculture and Forestry University Fuzhou350002China Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization at College of Landscape Architecture Fujian Agriculture and Forestry UniversityFuzhou350002China Shenzhen Key Laboratory for Orchid Conservation and Utilization The National Orchid Conservation Center of China and the Orchid Conservation and Research Center of ShenzhenShenzhen518114China

Cymbidium,which includes approximately 80 species,is one of the most ornamental and cultivated orchid ***,a lack of markers and sparse sampling have posed great challenges to resolving the phylogenetic relationships within the *** the present study,we reconstructed the phylogenetic relationships by utilizing one nuclear DNA(nrits)and seven plastid genes(rbcL,trnS,trnG,matK,trnL,psbA,and atpI)from 70 species(varieties)in *** also examined the occurrence of phylogenetic conflict between nuclear(nrits)and plastid loci and investigated how phylogenetic conflict bears on taxonomic classification within the *** found that phylogenetic conflict and low support values may be explained by hybridization and a lack of informative *** results do not support previous classification of the subgenera and sections within *** between gene trees and network analysis indicate that reticulate evolution occurred in the genus ***,our study indicates that Cymbidium has undergone a complex evolution.

关键词： Cymbidium Phylogenetic conflict nrits cpDNA Reticulate evolution

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青藏高原特有种黄缨菊谱系地理学研究

青藏高原特有种黄缨菊谱系地理学研究

作者：马子兰青海师范大学

学位级别：硕士

黄缨菊(Xanthopappus subacaulis)是菊科(Asteraceae)、黄缨菊属(Xanthopappus)的一种特有高山草甸药用植物,具有耐寒、耐旱、抗盐碱等诸多优良特性,尤其对维持高寒草甸生态系统稳定性有重要作用。迄今,国内外关于黄缨菊的研究主要集中... 详细信息

黄缨菊(Xanthopappus subacaulis)是菊科(Asteraceae)、黄缨菊属(Xanthopappus)的一种特有高山草甸药用植物,具有耐寒、耐旱、抗盐碱等诸多优良特性,尤其对维持高寒草甸生态系统稳定性有重要作用。迄今,国内外关于黄缨菊的研究主要集中在生物学特性、化学成分和药理活性、耐逆生理及系统位置等领域,然而采用分子标记对其进行群体遗传结构和进化历史的研究目前尚属空白。据此,本研究利用母系遗传的cpDNA(编码区rbc L和非编码区psb A-trn H、trn L-F和psb I-psb K)和双亲遗传的nrits序列,对青藏高原地区特有种黄缨菊44个居群、232个个体进行谱系地理学研究,探讨黄缨菊群体遗传结构和遗传多样性,推测其冰期避难所及种群历史动态,阐明青藏高原气候变迁对黄缨菊遗传变异分布式样和地理分布格局的影响,这些研究结果对于理解高山药用植物的进化历史具有重要启发意义。主要研究结果如下:(1)叶绿体DNA和核糖体nrits片段共检测到黄缨菊13种单倍型(H1-H13)和29种基因型(T1-T29);黄缨菊具有较高的遗传多样性(cpDNA:H=0.7010,π=0.0009,H=0.720;nrits:H=0.8430,π=0.0094,H=0.853),遗传变异主要存在于居群间(cpDNA:81.74%;nrits:86.06%),并且遗传分化程度较高(cpDNA:F=0.81736;nrits:F=0.86059),基因交流不频繁(cpDNA:N=0.11;nrits:N=0.08)。(2)群体遗传结构分析表明,黄缨菊存在较为明显的谱系地理结构(cpDNA:N=0.8090.05;nrits:N=0.861nrits:r=0.401,P=0.01)。(3)系统发育研究显示,黄缨菊44个居群以阿尼玛卿山为界划分为北部(Group 1)和南部(Group 2)两大谱系分支,与中央网络连接图、Split tree和STRUCTURE的聚类结果一致;单倍型分歧时间估算表明,黄缨菊南北两大谱系分支的分化时间为4.92 Ma(95%HPD:3.34-6.63 Ma),谱系内分化时间的分别为4.33 Ma(北部,95%HPD:2.94-5.89 Ma)和3.13 Ma(南部,95%HPD:1.81-4.47 Ma),谱系分化与中新世和上新世气候波动有关。(4)黄缨菊居群没有经历近期扩张,仅在第四纪冰期时经历居群收缩,第四纪冰期气候振荡和青藏高原抬升致使黄缨菊的最适分布区在末次盛冰期后面积骤缩、生境严重片段化;黄缨菊在青海湖地区、阿尼玛卿山和横断山等地可能存在多个冰期避难所,冰期后避难所内黄缨菊居群的二次接触应是黄缨菊形成现代地理分布格局的主要原因。

关键词：谱系地理学 nrits cpDNA 黄缨菊青藏高原

New record of luminescent Mycena chlorophos(Mycenaceae)from Western Ghats of India

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Studies in Fungi 2021年第1期6卷 507-513页

作者： Arya CP Ratheesh S Pradeep CK Microbiology Division Jawaharlal Nehru Tropical Botanic Garden&Research InstitutePalodeThiruvananthapuram695562KeralaIndia

An interesting luminescent Mycena was collected from dead bamboo culms on several occasions from an evergreen forest in Kerala State,*** morphological and molecular studies with nrits sequence data confirmed it as Mycena chlorophos.A reappraisal of the species along with comprehensive description,photographs and a discussion with related species is *** forms the first record of this species from India.

关键词： Bioluminescent new record nrits Omphalotaceae Pleurotaceae Physalacriaceae

九子母属的系统位置:来自叶绿体rbcL序列及核核糖体ITS序列的证据

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植物分类学报 2008年第4期46卷 586-594页

作者：潘跃芝龚洵杨杨中国科学院昆明植物研究所昆明650204

九子母属Dobinea Buch.-*** ***是一个东亚特有属,包含贡山九子母*** Buch.-*** ***和羊角天麻***(Baill.)Baill.两种,主要分布于中国西南地区。有关九子母属的系统位置长期存在争议,不同的学者曾将其归入漆树科Anacardiaceae、无患子科... 详细信息

九子母属Dobinea Buch.-*** ***是一个东亚特有属,包含贡山九子母*** Buch.-*** ***和羊角天麻***(Baill.)Baill.两种,主要分布于中国西南地区。有关九子母属的系统位置长期存在争议,不同的学者曾将其归入漆树科Anacardiaceae、无患子科Sapindaceae和九子母科Podoaceae。本文基于rbcL和ITS探讨了其系统位置,结果表明,九子母属的两个种自成单系,并且是漆树科的成员。

关键词：九子母属 nrits 系统位置 rbcL

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基于DNA序列的白头翁属植物自然杂交证据

西北植物学报 2011年第9期31卷 1738-1741页

作者：杨威邱爽金武烈吕惠子延边大学药学院吉林延吉133000 韩国忠北大学生命科学学院韩国全州561756

对长白山区兴安白头翁(Pulsatilla dahuricaSprengel)、朝鲜白头翁[***(Thunb.)Berchtold&Presl]以及其自然杂交种(***×***)进行ITS和psbA-trnH序列分析,结果表明,自然杂交种与兴安白头翁遗传距离为0;ITS电泳显示,在6个信息位点的峰值... 详细信息

关键词：白头翁自然杂交 nrits psbA-trnH

Umbilicariaceae科的姐妹种:进化意义和命名问题(英文)

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Umbilicariaceae科的姐妹种:进化意义和命名问题(英文)

2010全国地衣与苔藓系统学研讨会

作者： E.A.Davydov Altai State University Lenin Ave.61Barnaul656049Russia

Members of the Umbilicariaceae exhibit a wide range of propagation *** mycobionts may reproduce by ascospores or ***,lichenized propagules(reproduction of both mycobiont and photobiont),such as thallyles,lobes,isidia,... 详细信息

关键词： Evolution Phylogeny nrits mtLSU Umbilicaria Lasallia Thalloconidia

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两种独行菜序列特征差异比较

基因组学与应用生物学 2020年第6期39卷 2666-2670页

作者：钟江斌李威霖王婷都玉蓉马建滨青海师范大学生命科学学院青海省青藏高原生物多样性形成机制与综合利用重点实验室青海省青藏高原药用动植物资源重点实验室西宁810008

以宽叶独行菜和钝叶独行菜为材料,采用CTAB法提取总DNA,参照文献中引物对两物种nrits基因、trnL-trnF基因及psbA-trnH基因进行PCR扩增和测序,获得两物种的3个基因片段,并分析其序列特征。结果显示,nrits基因序列中A+T含量低于G+C含量,... 详细信息

以宽叶独行菜和钝叶独行菜为材料,采用CTAB法提取总DNA,参照文献中引物对两物种nrits基因、trnL-trnF基因及psbA-trnH基因进行PCR扩增和测序,获得两物种的3个基因片段,并分析其序列特征。结果显示,nrits基因序列中A+T含量低于G+C含量,而两叶绿体基因序列的碱基组成则相反,A+T含量显著高于G+C含量。用DNAMAN软件对两独行菜物种进行同源性分析,结果表明宽叶独行菜和钝叶独行菜nrits、trnL-trnF及psbA-trnH基因序列同源性分别为95.65%、97.97%及99.25%。本研究首次扩增获得宽叶独行菜和钝叶独行菜psbA-trnH基因序列,为独行菜属植物分子遗传学积累了研究资料。

关键词：宽叶独行菜钝叶独行菜 nrits trnL-trnF psbA-trnH 序列分析