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Asian Journal of Andrology 2011年第6期13卷 895-897页

作者： Giorgio Cavallini Giovanni Beretta Giulio Biagiotti Andros-Italia Outpatient Clinic of Ferrara Via Mascheraio 46 Ferrara 44121 Italy Centro Italiano Fertilita e Sessualita Via della Fortezza 6 Firenze 50129 Italy Andros Italia Sede di Perugia Via Martiri dei Lager 58 Perugia 06128 Italy

We investigated whether letrozole （2.5 mg day-1） improves sperm count in non-obstructive azoospermia （NOA） patients. Four men were included in this study, and they had folliculo-stimulating hormone and other hormone levels within the normal range and no varicoceles or chromosomal aberrations. These four patients were administered letrozole for 3 months. Sperm count, testicular volume, gonadotropin, testosterone （T） and estradiol （E2） blood levels were assessed before, during and 1 week after the suspension of treatment. All patients showed spermatozoa in their ejaculate, increased gonadotropin and T levels and lower E2 levels （P〈0.05 in all cases）, when letrozole was administered. This suggests that letrozole treatment might improve sperm count in an NOA sub-population; however, more studies, including the proper controls, are needed to confirm its efficacy.

关键词： letrozole medical treatment non-obstructive azoospermia spermatogenesis

RNAs in the testicular tissue of patients with non-obstructive azoospermia

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Asian Journal of Andrology 2022年第6期24卷 660-665页

作者： Zhe Zhang Han Wu Lin Zheng Hai-Tao Zhang Yu-Zhuo Yang Jia-Ming Mao De-Feng Liu Lian-Ming Zhao Hui Liang Hui jiang Department of Urology Peking University Third HospitalBeijing 100191China Department of Andrology Peking University Third HospitalBeijing 100191China Department of Reproductive Medicine Center Peking University Third HospitalBejing 100191China Department of Pathology School of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China

Circular RNAs(circRNAs)are highly conserved and ubiquitously expressed noncoding RNAs that participate in multiple reproductionrelated ***,the expression pattern and potential functions of circRNAs in the testes of patients with non-obstructive azoospermia(NOA)remain *** this study,according to a circRNA array,a total of 37881 circRNAs were identified that were differentially expressed in the testes of NOA patients compared with normal controls,including 19874 upregulated circRNAs and 18007 downregulated *** quantitative real-time polymerase chain reaction(qRT-PCR)analysis,we confirmed that the change tendency of some specific circRNAs,including hsa_circ_0137890,hsa_circ_0136298,and hsa_circ_0007273,was consistent with the microarray data in another larger *** structures and characteristics of these circRNAs were confirmed by Sanger sequencing,and fluorescence in situ hybridization revealed that these circRNAs were primarily expressed in the *** analysis was used to construct the competing endogenous RNA(ceRNA)network,and numerous miRNAs that could be paired with circRNAs validated in this study were reported to be vital for spermatogenesis *** Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses indicated that genes involved in axoneme assembly,microtubule-based processes,and cell proliferation were significantly *** data suggest that there are aberrantly expressed circRNA profiles in patients with NOA and that these circRNAs may help identify key diagnostic and therapeutic molecular biomarkers forNoA patients.

关键词： circular RNA microarray non-obstructive azoospermia spermatogenesis testicular tissue

A susceptibility locus rs7099208 is associated with non-obstructive azoospermia via reduction in the expression of FAM160B1

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The Journal of Biomedical Research 2015年第6期29卷 491-500页

作者： Yan Zhang Jing Qian Minghui Wu Mingxi Liu Kai Zhang Yuan Lin Xuejiang Guo Zuomin Zhou Zhibin Hu Jiahao Sha State Key Laboratory of Reproductive Medicine Nanjing Medical University The Center for Reproductive Medicine The Second Affiliated Hospital of Nanjing Medical University Department of Histology and Embryology Nanjing Medical University Department of Epidemiology and Biostatistics and Key Laboratory of Modern Toxicology of Ministry of Education Nanjing Medical University

non-obstructive azoospermia （NOA） is a severe defect in male reproductive health that occurs in 1% of adult men. In a previous study, we identified that rs7099208 is located within the last intron of FAM160B1 at 10q25.3. In this study, we analysed expression Quantitative Trait Loci （eQTL） of FAM16OB1, ABLIM1 and TRUB1, the three genes surrounding rs7099208. Only the expression level of FAM16OB1 was reduced for the homozygous alternate genotype （GG） of rs7099208, but not for the homozygous reference or heterozygous geno- types. FAM160B1 is predominantly expressed in human testes, where it is found in spermatocytes and round sper- matids. From 17 patients with NOA and five with obstructive azoospermia （OA）, immunohistochemistry revealed that expression of FAM160B1 is reduced, or undetectable in NOA patients, but not in OA cases or normal men. We conclude that rs7099208 is associated with NOA via a reduction in the expression of FAM160B1.

关键词： non-obstructive azoospermia obstructive azoospermia rs7099208 FAM160B1 expressionQuantitative Trait Loci apoptosis

Testicular fine needle aspiration for sperm retrieval in non-obstructive azoospermia

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Asian Journal of Andrology 2004年第1期6卷 58-58页

作者： YiGuo Ke-JunGuo DepartmentofGynecology ChinaMedicalUniversityFirstClinicalCollegeShenyang110001China

Objective: To investigate the feasibility of obtaining mature spermatozoa for intracytoplasmic sperm injection (ICSI) by testicular fine needle aspiration (TEFNA) in men diagnosed non-obstructive azoospermia. Methods: TEFNA was performed in 121 patients with a mean of 15 punctures and aspirations from each testis with a #23 butterfly needle connected to a 20 mL syringe with an aspiration handle. Results: One hundred and twenty-one patients underwent 176 TEFNA cycles. Testicular sperm were recovered in 56.3 % (99/176) cycles from 57 % (69/121) of patients. The sperm recovery rate was 46.7 % (21/45) in patients with Sertoli cell-only syndrome, 45.7 % (16/35) in patients with maturation arrest, 96.1 % (25/26) in patients with hypospermatogenesis and 63.6 % (7/11) in patients of non-mosaic Klinefelter's syndrome as judged by testicular histology. No sperm were found in 3 cases with post-irradiation fibrosis and one, after resection and chemotherapy of unilateral testicular cancer. In 87 cycles of ICSI using the husbands' sperm, 591 mature oocytes were injected, 218 (36.9 %) were normally fertilized and 202 embryos developed; 178 were transferred in 62 cycles resulting in 26 pregnancies (41.9 %) with 44 gestational sacs (implantation rate: 24.7 %). Conclusion: TEFNA was an efficient, easy to learn, safe and well tolerated treatment in patients with non-obstructive azoospermia.

关键词： fine needle aspiration intracytoplasmic sperm injection non-obstructive azoospermia

Multivariate analysis to predict letrozole efficacy in improving sperm count of non-obstructive azoospermic and cryptozoospermic patients： a pilot study

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Asian Journal of Andrology 2013年第6期15卷 806-811,I0009-I0010页

作者： Giorgio Cavallini Giulio Biagiotti Elisa Bolzon Andros-ltalia Outpatient Clinic of Ferrara Ferrara 44121 Italy Androsqtalia Outpatient Clinic of Perugia PeruRia 06128 Italy

We tested the hypothesis that letrozole increases sperm count in non-obstructive azoospermic or cryptozoospermic patients with a testosterone （T）/17-beta-2-oestradiol （E2） ratio 〈 10. Forty-six patients with no chromosomal aberrations were randomized into two groups： 22 received letrozole 2.5 mg per day for 6 months （Group 1：6 azoospermic＋ 16 cryptozoospermic patients）, while 24 received a placebo （Group 2：5 azoospermic＋19 cryptozoospermic patients）. The following data were collected： two semen analyses, clinical history, scrotal Duplex scans, body mass index （BMI）, Y microdeletion, karyotype and cystic fibrosis screens and follicle-stimulating hormone （FSH）, luteinizing hormone （LH）, E2, T and prolactin levels. Both before and after letrozole or placebo administration, the patients underwent two semen analyses and hormonal assessments. The differences were evaluated using the Mann-Whitney Utest. The relationships between sperm concentration after letrozole administration with respect to FSH, TIE2 ratio, bilateral testicle volume and BMI before letrozole administration were assessed using multivariate analysis. The side effects were assessed using the chi-square test. Group 1 had sperm concentration （medians： 400-1.290× 10^6 ml^-1; P〈0.01） and motility （medians： class A from 2% to 15%; P〈0.01）, FSH, LH and T significantly increased, while Group 2 did not. E2 levels diminished significantly in Group 1, but not in Group 2. Eight patients in Group 1 demonstrated side effects, whereas no patient side effects were observed in Group 2. The sperm concentration after letrozole administration is inversely related to TIE2, FSH and BMI; a direct relationship emerged between sperm concentration and testicular volume.

关键词： cryptozoospermia letrozole male infertility non-obstructive azoospermia

Microdissection testicular sperm extraction： an update

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Asian Journal of Andrology 2013年第1期15卷 35-39页

作者： Ali A Dabaja Peter N Schlegel Department of Urology Male Reproductive Medicine Weill Cornell Medical College New York NY 10065 USA

Patients with non-obstructive azoospermia （NOA） were once considered to be infertile with few treatment options due to the absence of sperm in the ejaculate. In the last two decades, the advent of intracytoplasmic sperm injection （ICSI）, and the application of various testicular sperm retrieval techniques, including fine needle aspiration （FNA）, conventional testicular sperm extraction （TESE） and microdissection testicular sperm extraction （micro-TESE） have revolutionized treatment in this group of men. Because most men with NOA will have isolated regions of spermatogenesis within the testis, studies have illustrated that sperm can be retrieved in most men with NOA, including Klinefelter＇s syndrome （KS）, prior history of chemotherapy and cryptorchidism. Micro-TESE, when compared with conventional TESE has a higher sperm retrieval rate （SRR） with fewer postoperative complications and negative effects on testicular function. In this article, we will compare the efficacy of the different procedures of sperm extraction, discuss the medical treatment and the role of testosterone optimization in men with NOA and describe the micro-TESE surgical technique. Furthermore, we will update our overall experience to allow counseling on the prognosis of sperm retrieval for the specific subsets of NOA.

关键词： azoospermia genetics male infertility microsurgery non-obstructive azoospermia sperm retrieval testicular sperm extraction

Testis sperm extraction

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Asian Journal of Urology 2015年第2期2卷 79-84页

作者： Kirsten J.C.Janosek-Albright Peter N.Schlegel Ali A.Dabaja Department of Urology Vattikuti Urology InstituteHenry Ford HospitalDetroitMIUSA Department of Urology Weill Cornell Medical CollegeNew YorkNYUSA

The last 20 years have produced developments in the treatment for patients with non-obstructive azoospermia(NOA)who were once considered to be *** combination of intracytoplasmic sperm injection together with various testicular sperm retrieval techniques,including conventional testicular sperm extraction(TESE),microdissection TESE(micro-TESE)and fine needle aspiration(FNA),have revolutionized treatment for these *** men with NOA,isolated regions of spermatogenesis within the testis are *** goal for all types of sperm retrieval procedures is locating the focal region(s)of spermatogenesis,and harvesting the sperm for assisted *** review article explores the surgical management of men with NOA and describes all techniques that can be used for testicular sperm retrieval.A PubMed search was conducted using the key words:“sperm extraction”,“NOA”,“testicular FNA”,“testicular mapping”,“TESE”,and“testicular biopsy”.All articles were *** were included if they provided data on sperm retrieval *** methods for performing sperm retrieval rates and outcomes of the various techniques are ***-TESE has a higher sperm retrieval rates with fewer postoperative complications and negative effects on testicular function compared with conventional TESE.

关键词： non-obstructive azoospermia Microdissection testicular sperm extraction Fine needle aspiration Sperm retrieval

Proposed age-stratified reference intervals of FSH derived from normozoospermic men

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Asian pacific Journal of Reproduction 2021年第4期10卷 162-167页

作者： Emily-Jane Waller Jason Conceicao Phillip Matson John L Yovich School of Medical and Health Sciences Edith Cowan UniversityJoondalupPerthAustralia PIVET Medical Centre LeedervillePerthAustralia School of Pharmacy and Biomedical Sciences Faculty of Health SciencesCurtin UniversityBentleyPerthAustralia

Objective:To demonstrate that serum follicle-stimulating hormone(FSH)in men rises with age,and to explore FSH reference intervals of age-related ***:Men aged 20-50 years(n=1190)underwent semen analysis according to World Health Organization(2010)*** was frozen prior to measurement of FSH by using the Siemens ADVIA Centaur®XP immunoassay *** central 95%intervals after logarithmic transformation based on age were derived from 1037 normozoospermic *** were then applied to oligozoospermic and azoospermic *** producing azoospermic semen samples were further classified as having non-obstructive azoospermia by clinical diagnostic criteria,including genetic analysis and surgical ***:Serum FSH in normozoospermic men increased with age(Pazoospermia as either obstructive or non-obstructive azoospermia,and 86.4%(38/44)men with non-obstructive azoospermia had elevated serum FSH concentrations whereas only 6.7%(1/15)men with obstructive azoospermia had high FSH levels,and this was significantly different(P<0.01).Conclusions:FSH concentrations increase in men between 20-50 years,and clinical interpretation of serum FSH results in men must be made by using age-based reference intervals.

关键词： Male infertility FSH Reference intervals Age obstructive azoospermia non-obstructive azoospermia

The number of spermatozoa collected with testicular sperm extraction is a novel predictor of intracytoplasmic sperm injection outcome in non-obstructive azoospermic patients

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Asian Journal of Andrology 2011年第2期13卷 312-316页

作者： Giorgio Cavallini Maria Cristina Magli Andor Crippa Silvia Resta Giovanni Vitali Anna Pia Ferraretti Luca Gianaroli Andros-ltalia Outpatient Clinic of Ferrara 44121 Ferrara Italy Operative Unit of Laboratory of Assisted Reproduction Societe Italiana di Studi di Medicina della Riproduzione（S.I.S.ME.R.） 40138 Bologna Italy Operative Unit of Andrology Societ Italiana di di Studi di Medicina della Riproduzione 40138 Bologna Italy a Operative Unit of AssistedReproduction Societ＆ Italiana di di Studi di Medicina della Riproduzione 40138 Bologna Italy

The purpose of this study was to determine the relationships between monitors of spermatogenesis and predictors of the intracytoplasmic sperm injection （ICSI） outcome in patients with non-obstructive azoospermia （NOA） undergoing testicular sperm extraction （TESE）. Seventy-nine patients with NOA （mean age： 43.6±5.2 years）, each of whom yielded （97 000±3040） spermatozoa with conventional TESE, were considered in our analysis. Their partners （mean age： 35.8±5.1 years） underwent a total of 184 ICSI cycles; 632 oocytes were collected, 221 oocytes were injected, 141 oocytes were fertilized, 121 embryos were obtained, 110 embryos were transferred, 14 clinical pregnancies were achieved and only one miscarriage occurred. Multivariate regression analysis indicated relationships between the percentage of fertilized oocytes, transferred embryos and clinical pregnancies with the following variable values： female partner＇s age, number of spermatozoa collected, testicular volume, male partner＇s levels of follicle stimulating hormone （FSH）, number of oocytes collected, number of oocytes injected and number of ICSI cycles. A significant inverse relationship was found between female partner＇s age or male partner＇s FSH levels and biochemical pregnancies. A significant direct relationship emerged between the number of ICS! cycles and the percentage of oocytes fertilized, embryos transferred and biochemical pregnancies, and between the number of spermatozoa collected per testicular biopsy and biochemical pregnancies. The number of spermatozoa was positively linked to the number of clinical pregnancies, independent of the number of ICSI cycles and the number of oocytes collected/injected. The number of spermatozoa collected, FSH level and testicular volume are monitors of spermatogenesis linked to ICSI success.

关键词： conventional TESE ICSI outcomes non-obstructive azoospermia

Genetic basis of meiotic abnormalities in non-obstructive azoospermic patients

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Genetic basis of meiotic abnormalities in non-obstructive az...