检索结果-南通市图书馆

Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading chinese cabbage with transgenic roots

引用

Horticultural Plant Journal 2024年第2期10卷 450-460页

作者： Huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou State Key Laboratory of Crop Genetics&Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNanjingJiangsu 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNanjingJiangsu 210095China

non-heading chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu *** one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading chinese cabbage genetic improvement breeding.

关键词： Agrobacterium rhizogenes non-heading chinese cabbage Transgenic roots Composite plant Transformation efficiency

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The construction of a genetic linkage map of non-heading chinese cabbage (Brassica campestris *** Makino)

引用

Journal of Genetics and Genomics 2009年第8期36卷 501-508页

作者： Yan Cheng Jianfeng Geng Jingyi Zhang Qian Wang Qingyu Ban Xilin Hou State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China Key laboratory of Southern Vegetable Crop Genetic Improvement Ministry ofAgriculture Nanjing Agricultural University Nanjing 210095 China Institute of Horticulture Henan Academy of Agricultural Science Zhengzhou 450002 China

non-heading chinese cabbage （Brassica carnpestris ssp. chinensis Makino） is one of the most important vegetables in eastern China. A genetic linkage map was constructed using 127 doubled haploid （DH） lines, and the DH population was derived from a commercial hybrid ＂Hanxiao＂（lines SW-13 x L-118）. Out of the 614 polyrnorphic markers, 43.49% were not assigned to any of the linkage groups （LGs）. Chi-square tests showed that 42.67% markers were distorted from expected Mendelian segregation ratios, and the direction of distorted segregation was mainly toward the paternal parent L-118. After sequentially removing the markers that had an interval distance smaller than 1 cM from the upper marker, the overall quality of the linkage map was increased. Two hundred and sixty-eight molecular markers were mapped into 10 LGs, which were anchored to the corresponding chromosome of the B. rapa reference map based on com- mon simple sequence repeat （SSR） markers. The map covers 973.38 cM of the genome and the average interval distance between markers was 3.63 cM. The number of markers on each LG ranged from 18 （R08） to 64 （R07）, with an average interval distance within a single LG from 1.70 cM （R07） to 6.71 cM （R06）. Among these mapped markers, 169 were sequence-related amplified polymorphism （SRAP） molecular markers, 50 were SSR markers and 49 were random amplification polymorphic DNA （RAPD） markers. With further saturation to the LG9 the current map offers a genetic tool for loci analysis for important agronomic traits.

关键词： non-heading chinese cabbage genetic linkage map SRAP SSR RAPD

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Molecular Cloning,Expression Analysis and Localization of Exo70A1 Related to Self Incompatibility in non-heading chinese cabbage(Brassica campestris ***)

引用

Journal of Integrative Agriculture 2013年第12期12卷 2149-2156页

作者： WANG Li GE Ting-ting PENG Hai-tao WANG Cheng LIU Tong-kun HOU Xi-lin LI Ying Horticultural Department Nanjing Agricultural University State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Southern Vegetable Crop Genetic Improvement Ministry of Agriculture

The exocyst is a conserved protein complex,and required for vesicles tethering,fusion and polarized ***70A1,the exocyst subunit,is essential for assembly of the exocyst *** better understand potential roles of Exo70A1 in non-heading chinese cabbage（Brassica campestris ***）,we obtained the full-length cDNA of Exo70A1 gene,which consisted of 1 917 bp and encoded a protein of 638 amino *** showed BcExo70A1 shared 94.9% identity with Brassica oleracea ***（AEI26267.1）,and clustered into a same group with other homologues in *** *** and Brassica *** localization analysis showed BcExo70A1 was localized to punctate structures in cytosol of onion epithelial *** showed that BcExo70A1 was widely presented in stamens,young stems,petals,unpollinated pistils,roots and leaves of self compatible and incompatible *** transcripts of BcExo70A1 in non- heading chinese cabbage declined during initial 1.5 h after incompatible pollination,while an opposite trend was presented after compatible *** study reveals that BcExo70A1 could play essential roles in plant growth and development,and is related to the rejection of self pollen in non-heading chinese cabbage.

关键词： Exo70A1 gene expression subcellular localization self-incompatibility non-heading chinese cabbage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Isolation and Characterization of a Novel Chitosan-Binding Protein from non-heading chinese cabbage Leaves

引用

Journal of Integrative Plant Biology 2005年第4期47卷 452-456页

作者： Hui-PingCHEN Lang-LaiXU CollegeofLifeSciences NanfingAgriculturalUniversityNanjing210095China／／CollegeofAgronomySouthChinaTropicalAgriculturalUniversityDanzhou571737China CollegeofLifeSciences NanfingAgriculturalUniversityNanjing210095China

Abstract: To know the mechanism of ammonia assimilation in non-heading chinese cabbage (Brassica campestrish L. ssp. chinensis (L.) Makino) leaves regulated by chitosan (CTS), a CTS-binding protein was isolated from non-heading chinese cabbage leaves using the chitosan affinity chromatography approach and this CTS-binding protein was partially characterized. The profile of the 53.1 kDa purified protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was compared with the native molecular weight of 106.5 kDa, which indicated that the purified protein was a dimer with identical subunits. After isoelectric focusing, a band was obtained at pH 8.25. The agglutination test and periodic acid-Schiff staining further revealed that the protein was a glycoprotein with lectin activity. Moreover, the purified protein contained 17.4% (w/w) neutral carbohydrate and 82.56% (w/w) protein. The comparison of this protein and the 67 kDa CTS-binding protein isolated previously from Rubus culture tissue exhibited some differences in characterization. According to results of peptide mass fingerprinting analysis, the protein purified in the present study does not show any similarity with any protein in the protein data bank. Thus, it was deduced that the protein purified in the present study is a novel CTS-binding protein.

关键词： binding-protein chitosan non-heading chinese cabbage

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Using a Suppression Subtractive Library-Based Approach to Identify non-heading chinese cabbage Genes Up-Regulated in Early Response to Elicitor PB90

引用

Agricultural Sciences in China 2008年第3期7卷 303-313页

作者： GAN Yun-zhe ZHANG Zheng-guang WANG Yuan-chao ZHENG Xiao-bo Key Lab of Monitoring and Management of Plant Disease and Insects Ministry of Agriculture/College of Plant Protection Nanjing Agricultural University Nanjing 210095 P.R.China

Monitoring expression at the transcriptional level is the first essential step for the functional analysis of plant genes. Genes-encoding proteins directly involved in early response to elicitor constitute only a small fraction of all the genes affected by elicitor. Transcriptional responses to various elicitors have been extensively studied in different plants including Nicotiana and Arabidopsis thaliana; however, corresponding data aren＇t available for non-heading chinese cabbage. To address this problem, we describe a suppression subtractive library-based approach to isolate the plant＇s ESTs up-regulated in the early induction/execution of the HR induced by elicitor PB90 from Phytophthora boehmeriae. According to their putative identification in BLAST searches against the three genome databases, 70 up-regulated genes were classified into 9 parts： some aspect of primary ＇metabolism＇ or ＇energy＇ production; ＇protein synthesis＇ or ＇protein fate＇; cellular communication/signal transduction mechanism; cell fates including Beclin, SPT1, and SPT2; HLA-B and AGO1 which participate in transcription; cellular transport and hypothetical proteins or proteins for which a function has yet to be determined. Seven selected genes such as Beclin, thioredoxin, HLA-B, MAP3K, SPT1, SPT2, and AGO1 were up-regulated induced by PB90, suggesting that the genes may play an important role in PB90-triggered HR.

关键词： PB90 non-heading chinese cabbage suppression subtractive hybridization up-regulated hypersensitive response

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of Partial Replacement of Nitrate by Amino Acid and Urea on Nitrate Content of non-heading chinese cabbage and Lettuce in Hydroponic Condition

引用

Agricultural Sciences in China 2002年第4期1卷 444-449页

作者： CHEN Gui-lin, GAO Xiu-rui and ZHANG Xian-bin( College of Horticulture , Agricultural University of Hebei, Baoding 071001 , P. R . China ) College of Horticulture Agricultural University of Hebei Baoding 071001 P.R. China

In this paper, the authors studied the effect of different mixtures of glycine (Gly), isoleucine (Iso), proline (Pro), and urea solutions used as a partial (20%) replacment of nitrate in the nitrate content and quality of non-heading chinese cabbage and lettuce in hydroponice. Five treatments were done 12 d before harvest. Compared to the control group, Gly had the best effect in reducing the nitrate content of both vegetable leaves and petioles; the mixture of Gly, Iso and Pro ranked second and urea the least. Treatments with amino acid could also increase soluble sugar and protein contents and enhance total-N in leaves significantly. In contrast, amino acid enhanced NRA in non-heading chinese cabbage, while they decreased it slightly in lettuce. The results showed that amino acids and urea could reduce the nitrate content of both vegetables, but they had almost the same effect on non-heading chinese cabbage. Moreover, amino acids were more effective than urea in lettuce. As a result, it was concluded that partial replacement of nitrate with amino acids not only reduced the nitrate content but also improved the quality of vegetables.

关键词： non-heading chinese cabbage Lettuce Hydroponice Nutrient solution Amino acid Nitrate

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Fine mapping and cloning of the sterility gene Bra2Ms in nonheading chinese cabbage(Brassica rapa ***)

引用

Journal of Integrative Agriculture 2024年第4期23卷 1195-1204页

作者： Liping Song Xia Li Liguang Tang Chuying Yu Bincai Wang Changbin Gao Yanfeng Xie Xueli Zhang Junliang Wang Chufa Lin Aihua Wang Wuhan Vegetable Research Institute Wuhan Academy of Agricultural SciencesWuhan 430345China Biotechnology and Genetic Resources Institute Yunnan Academy of Agricultural SciencesKunming 650000China

The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading chinese cabbage(Brassica rapa ***).However,the molecular mechanisms underlying male sterility in *** are still largely *** previously obtained the natural male sterile line WS24-3 of non-heading chinese cabbage and located the male sterile locus,Bra2Ms,on the A2 *** observations revealed that the male sterility of WS24-3 resulted from disruption of the meiosis process during pollen *** mapping of Bra2Ms delimited the locus within a physical distance of about 129 kb on the A2 chromosome of *** Bra039753 gene encodes a plant homeodomain(PHD)-finger protein and is considered a potential candidate gene for ***039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther *** analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants,which led to an amino acid insertion in the Bra039753 *** addition,the 369 bp fragment insertion was found to cosegregate with the male sterility *** study identified a novel locus related to male sterility in non-heading chinese cabbage,and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading chinese cabbage and other Brassica crops.

关键词： non-heading chinese cabbage male sterility Bra2Ms fine mapping PHD-finger protein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：