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Plantlet regeneration of adult Pinus massoniana Lamb. trees using explants collected in March and thidiazuron in culture medium

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Journal of Forestry Research 2017年第6期28卷 1165-1171页

作者： Yin Wang Ruiling Yao Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation Guangxi Forestry Research InstituteNanning 530002 Guangxi People’s Republic of China

A protocol for micropropagation using nodal explants from mature Pinus massoniana trees has been developed. Time of explant collection is crucial for the initial success of aseptic culture. Explants collected in early March gave the highest percentage of explant survival (64.5%) and shoot-forming percentage (52.3%). Thidiazuron (TDZ) concentration significantly influenced shoot formation; 4 mu M TDZ was optimum, with 4.8 shoots produced per explant with a mean length of 7.1 cm after 120 days of culture. Regenerated shoots rooted for 60 days in basic medium with 1 mu M NAA were ready for growth in pots. This is the first report on plantlet regeneration in vitro from mature trees of P. massoniana that provides a reliable method for propagating selected elites.

关键词： Thidiazuron nodal segment Adventitious root Acclimatization NAA

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Artificial Seed Production of Tylophora indica for Interim Storing and Swapping of Germplasm

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Horticultural Plant Journal 2017年第1期3卷 41-46页

作者： Saikat Gantait Joshitha Vijayan Adity Majee Department of Genetics and Plant Breeding Faculty of AgricultureBidhan Chandra Krishi Viswavidyalaya Department of Agricultural Biotechnology Faculty Centre for Integrated Rural Development and ManagementSchool of Agriculture and Rural DevelopmentRamakrishna Mission Vivekananda University

Our research work demonstrates the single bead alginate-encapsulation, interim storing and conversion of Tylophora indica(Burm. Fil.) *** effective encapsulation of in vitro nodal segments [(4 ± 1) mm long], ensuing in sphere-shaped artificial seeds of similar morphology, was achieved through 75 mmol·L^(-1)calcium chloride(CaCl_2 · 2H_2O) plus 3%(w/v) Na-alginate with 93.3% conversion frequency. The earliest conversion(within 7 days of incubation) of artificial seeds occurred in half-strength liquid Murashige and Skoog medium. Among the three different temperature regimes [(5 ± 1) °C,(15 ± 1) °C, and(25 ± 1) °C], storage of artificial seeds at(15 ± 1) °C executed the highest frequency of conversion(90%) after 15 days of storage. Lengthier storage significantly reduced the conversion frequency of artificial seeds irrespective of storage temperature. Nevertheless, the conversion frequency after 30 days of storage at(15 ± 1) °C was recorded at 70% without further decline even following45 days of storage, which evidently suggests that lower temperature(15 ± 1) °C is apt for storage and subsequent conversion of T. indica artificial seeds. The present protocol could be expedient for short-term storing and swapping of T. indica germplasms between national and international laboratories.

关键词： Tylophora indica calcium chloride conversion encapsulation germplasm swap Na-alginate nodal segment

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