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Pro-catabolic effects of the mycotoxin nivalenol in bovine articular chondrocytes

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Pro-catabolic effects of the mycotoxin Nivalenol in bovine a...

Summary of Effects on Aggrecan Metabolism of Chondrocytes by nivalenol and Selenium

第十一届中国生物毒素研究及医药应用年会

作者： Siyuan Li Jinghong Chen 曹峻岭 Emma J Blain Bruce Caterson Victor C Duance The Department of Anaesthesia The Second Hospital of Xi'an Jiaotong University Key Laboratory of Environment and Genes Related to Diseases(Xi'an Jiaotong University) Ministry of Education Arthritis Research UK Biomechanics and Bioengineering Centre School of BiosciencesCardiff UniversityMuseum AvenueCardiffUKCF10 3AX

＜正＞Objective:Kashin-Beck Disease（KBD） is an endemic,age-related degenerative osteoarthritis and its cause is hypothesised to involve Fusarium mycotoxins,commonly associated with inappropriate storage of *** study sought to investigate the Fusarium mycotoxin nivalenol（NIV）,widely distributed in the cereals of KBD afflicted areas,on the metabolism of articular chondrocytes in vitro. Methods:The effect of 0.1,0.2 or 0.5μg/ml NIV,on primary bovine articular chondrocyte matrix metabolism was investigated using quantitative PCR to analyse transcript levels of matrix proteins *** I and II collagen,aggrecan,matrix metalloproteinases （MMPs）,aggrecanases（ADAMTS） and the tissue inhibitors of MMPs（TMPs）.Amounts of sGAG,MMPs and TIMPs released by the chondrocytes were assessed using the Dimethylmethylene Blue assay,gelatin zymography and reverse gelatin zymography *** element organisation was analysed using immunofluorescence in conjunction with scanning laser confocal microscopy,and expression levels measured by quantitative PCR and Western blot analysis. Results:A NIV dose-dependent increase in aggrecan transcription,concomitant with sulphated glycosaminoglycan retention in the cell lysate was ***,NIV significantly increased MMPs 2,-3 and -9,ADAMTS-4 and -5,and TIMPs 2 and -3 transcript *** contrast,NIV inhibited typeⅠcollagen,MMP 1 and TMP 1 mRNA *** promoted extensive cytoskeletal network remodelling,particularly evident for vimentin where there was dose-dependent peri-nuclear aggregation. Conclusion:Our studies have shown that NIV decreased matrix deposition,whilst enhancing catabolic enzyme production,suggesting its potential pro-catabolic ability in *** hypothesise that the observed reduction in matrix production may be attributed to the extensive remodelling and/or disassembly of the cytoskeletal elements in response to ***,these findings support the hypothesis that trichothecen

关键词： articular chondrocytes nivalenol extracellular matrix matrix metalloproteinases cytoskeleton Kashin-Beck Disease

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海外英语 2012年第20期 287-288页

作者： CAO Pei-hua1,CAO Jun-ling2,CAO Li-min1,LI Ren-ning1(1.Xi’an Medical University,Xi’an 710021,China 2.Institute of Endemic Diseases,Environment Related Gene Key Laboratory of Ministry of Edu cation,Xi’an Jiaotong University,Xi’an 710061,China)

From this article,we can find that nivalenol could damage the DNA,disturb aggrecan mRNA expression in chondro cytes and further induce metabolic disturbance of cartilage extracellular matrix,which may play an important role during patho genesis of *** supplementation can resist the injury of nivalenol to chondrocytes,but its action is limited.

关键词： chondrocytes nivalenol selenium aggrecan

The Effect of nivalenol on Metabolism of Cultured Chondrocytes and the Protection of Selenium

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Journal of Nanjing Medical University 2003年第3期17卷 97-105页

作者：楚瑞琦曹峻岭张军芳谢龙岳燕胡小平李思远谭升顺西安交通大学医学院第二医院皮肤科

Objective: To investigate the possible effect of nivalenol on metabolism ofthe cultured chondrocytes and the protection of selenium. Methods: The quantitative analyses ofmetabolism in single- layer cultured chondrocytes were performed by biocliemical means and theimpairment of DNA was observed by both of the single cell microgel electrophoresis assay and theagarose gel electrophoresis assay. Results: In the media containing different concentrations ofnivalenol (0. 000 5-0. 020 0 mg/L), the amounts of DNA and proteoglycan in matrix of thechondrocytes were decreased. The syn-thesis of protein was reduced and the impairment of DNAdeteriorated with the increase of the concentrations of nivalenol in tlte given dose. When seleniumwas added into the media, the impairment by nivalenol was decreased. In the media containingdifferent concentrations of nivalenol, however, the lipid peroxidation of the chondrocytes was notaffected by nivalenol, yet the amount of lipid peroxides significantly declined. Conclusion:nivalenol may evidently cause impairment of the chondrocytes when its concentrations are in thepresent experimental range. Selenium can protect cultured cliondrocytes, but cannot prevent theirDNA from being impaired.

关键词： nivalenol chondrocyte selenium

THE EXPERIMENTAL STUDY ON THE EFFECTS OF SIX MYCOTOXINS ON THE CULTURAL CHONDROCYTES

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Journal of Pharmaceutical Analysis 1998年第1期12卷 1-8页

作者：曹峻岭熊咏民李斯纯郑滨张矢远毕华银莫东旭 Institute of Endemic Bone Diseases Xi'an Medical University Xi'an 710061 China

The effects of deoxynlvalenol (DON), T--2 toxin, nivalenol (NIv), hutenolide (BuT).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyte monolayer culture. The amounts or DNA in chondrocytesand glucuronate in matrix were measured. And the chondrocytes were observed by inversion microscope and transmission electron microscope (TEM). The results showed that the cultured chondrocytes were damaged by all the six mycotoxinsl and the synthesis of DNA and the divided reproductionof chondrocytes were restrained; the damage errect was more evident, esl,ecially in the early stage ofculturel the higher concentration or toxin in the media was used, the lower density of the culturalckondrocytes was observed; the cells were even round damaged and dead, so long as the media contolued toxin. When the six mycotoxins arrected the cultural chondrocytes ***, three dirfereut kinds or ultrastructural changes in ckondrocytes were seen by TEa. The relationship betweenmycotoxiu and KBD was preliminarily discussed, and some problems still need further investigation..

关键词： chondrocyte culture deoxynivalenol T-2 toxin nivalenol butenolide alternariol methyl ether moniliformin Kashin-Beck disease