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Bamboo charcoal addition enhanced the nitrogen removal of anammox granular sludge with COD:Performance,physicochemical characteristics and microbial community

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Journal of Environmental Sciences 2022年第5期34卷 55-64页

作者： Menglei Guo Ying Jiang Junxiang Xie Qianfei Cao Qun Zhang Adams Mabruk Chongjun Chen School of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou 215009China Jiangsu Provincial Key Laboratory of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China

The effects of different chemical oxygen demand(COD)concentrations on the anammox granular sludge with Bamboo Charcoal(BC)addition were evaluated in UASB reactor.The results showed that the average total nitrogen(TN)removal efficiency was reduced from 85.9%to 81.4%when COD concentration was increased from 50 to 150 mg/L.However,the TN removal efficiency of BC addition reactors was dramatically 3.1%-6.4%higher than that without BC under different COD concentrations.The average diameter of granular sludge was 0.13 mm higher than that without BC.The settling velocity was increased by elevated COD concentration,while the EPS and VSS/SS were increased with BC addition.The high-throughput Miseq sequencing analyses revealed that the bacterial diversity and richness were decreased under COD addition,and the Planctomycetes related to anammox bacteria were Candidatus Brocadia and Candidatus Kuenenia.The Metagenomic sequencing indicated that the abundance of denitrification related functional genes all increased with elevated COD,while the abundance of anammox related functional genes of decreased.The func-tional genes related to anammox was hydrazine synthase encoding genes(hzsA,hzsB and hzsB).The average relative abundance of hzs genes in the reactor with BC addition was higher than the control at COD concentrations of 50 mg/L and 150 mg/L.The functional genes of denitrification mediated by BC were higher than those without BC throughout the operation phase.It is interesting to note that BC addition greatly enriched the related func-tional genes of denitrification and anammox.

关键词： Anammox granular sludge Bamboo charcoal COD concentration nitrogen removal Microbial community

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Enhanced nitrogen removal at low temperature with mixed anoxic/oxic process

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Water Science and Engineering 2023年第1期16卷 67-75页

作者： Xiang-kun Li Ying-jun Yang Gai-ge Liu Dou-dou Sun Xiao-chen Ma School of Civil and Transportation Engineering Hebei University of TechnologyTianjin 300401China School of Environment Harbin Institute of TechnologyHarbin 150090China

Different hydraulic retention times(HRTs)were tested in a mixed anoxic/oxic(A/O)system at 5C and 10C to investigate the effects of HRT and carrier on nitrogen removal in wastewater at low temperatures.The results showed that the addition of the fillers improved the treatment effect of each index in the system.With an optimal HRT of 7.5 h at 5C,the removal efficiencies of NHþ4-N and total nitrogen(TN)reached 91.2%and 75.6%,respectively.With an HRT of 6 h at 10C,the removal efficiencies of NHþ4-N and TN were 96.7%and 82.9%,respectively.The results of high-throughput sequencing showed that the addition of the suspended carriers in the aerobic zone could improve the treatment efficiency of nitrogen at low temperatures.The microbial analysis indicated that the addition of the suspended carriers enhanced the enrichment of nitrogen removal bacteria.Nitrospira,Nitrotoga,and Nitrosomonas were found to be the bacteria responsible for nitrification,and their relative concentrations on the biofilm at 5C and 10C accounted for 98.11%,92.79%,and 69.98%of all biological samples,respectively.

关键词： Domestic sewage Low temperature Mixed A/O process nitrogen removal Bacterial community structure

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Effect of wetland plant fermentation broth on nitrogen removal and bioenergy generation in constructed wetland-microbial fuel cells

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Frontiers of Environmental Science & Engineering 2022年第12期16卷 89-100页

作者： Yiting Chen Jun Yan Mengli Chen Fucheng Guo Tao Liu Yi Chen Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment(Ministry of Education) Chongqing UniversityChongqing400045China College of Environment and Ecology Chongqing UniversityChongqing400045China

Constructed wetlands(CWs)are widely used as a tertiary treatment technology,and the addition of carbon sources can significantly improve advanced nitrogen removal.However,excessive carbon sources would lead to an increase in the effluent chemical oxygen demand in CWs,and microbial fuel cells(MFCs)can convert these into electricity.In this study,constructed wetland-microbial fuel cells(CW-MFCs)were built to achieve simultaneous nitrogen removal and electricity generation,using wetland plant litter fermentation broths as carbon sources.The total nitrogen removal in the groups with fermentation broth addition(FGs)reached 83.33%,which was 19.64%higher than that in the CG(group without fermentation broth),and the mean voltages in the FGs were at least 2.6 times higher than that of the CG.Furthermore,two main components of the fermentation broths,acetic acid(Ac)and humic acid(HA),were identified using a three-dimensional excitation emission matrix and gas chromatograph and added to CW-MFCs to explore the influence mechanism on the treatment performance.Denitrification and electrogenesis presented the same tendency:Ac&HA>Ac>CG’(groups without Ac and HA).These results indicate that Ac and HA increased the abundance of functional genes associated with nitrogen metabolism and electron transfer.This study demonstrated that CW-MFC fermentation broth addition can be a potential strategy for the disposal of secondary effluent and bioelectricity generation.

关键词： Constructed wetland Microbial fuel cell nitrogen removal Bioenergy generation Carbon source

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nitrogen removal from wastewater and bacterial diversity in activated sludge at different COD/N ratios and dissolved oxygen concentrations

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Journal of Environmental Sciences 2012年第6期24卷 990-998页

作者： Magdalena Zielińska Katarzyna Bernat Agnieszka Cydzik-Kwiatkowska Joanna Sobolewska Irena Wojnowska-Baryla Department of Environmental Biotechnology University of Warmia and Mazury in OlsztynS oneczna St.45G10-709 OlsztynPoland

The impact of the organic carbon to nitrogen ratio （chemical oxygen demand （COD）/N） in wastewater and dissolved oxygen （DO） concentration on carbon and nitrogen removal efficiency, and total bacteria and ammonia-oxidizing bacteria （AOB） communities in activated sludge in constantly aerated sequencing batch reactors （SBRs） was determined. At DO of 0.5 and 1.5 mg O2/L during the aeration phase, the efficiency of ammonia oxidation exceeded 90%, with nitrates as the main product. Nitrification and denitrification achieved under the same operating conditions suggested the simultaneous course of these processes. The most effective nitrogen elimination （above 50%） was obtained at the COD/N ratio of 6.8 and DO of 0.5 mg O2/L. Total bacterial diversity was similar in all experimental series, however, for both COD/N ratios of 6.8 and 0.7, higher values were observed at DO of 0.5 mg O2/L. The diversity and abundance of AOB were higher in the reactors with the COD/N ratio of 0.7 in comparison with the reactors with the COD/N of 6.8. For both COD/N ratios applied, the AOB population was not affected by oxygen concentration. Amplicons with sequences indicating membership of the genus Nitrosospira were the determinants of variable technological conditions.

关键词： nitrogen removal activated sludge COD/N ratio oxygen concentration ammonia-oxidizing bacteria microbial diversity

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nitrogen removal influence factors in A/O process and decision trees for nitrification/denitrification system

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Journal of Environmental Sciences 2004年第6期16卷 901-907页

作者： MAYong PENGYong-zhen WANGShu-ying WANGXiao-lian SchoolofMunicipalandEnvironmentalEngineering HarbinInstituteofTechnologyHarbin150090China DepartmentofEnvironmentalEngineering BeijingUniversityofTechnologyBeijing100022China

In order to improve nitrogen removal in anoxic/oxic(A/O) process effectively for treating domestic wastewaters, the influence factors, DO(dissolved oxygen), nitrate recirculation, sludge recycle, SRT(solids residence time), influent COD/TN and HRT(hydraulic retention time) were studied. Results indicated that it was possible to increase nitrogen removal by using corresponding control strategies, such as, adjusting the DO set point according to effluent ammonia concentration; manipulating nitrate recirculation flow according to nitrate concentration at the end of anoxic zone. Based on the experiments results, a knowledge-based approach for supervision of the nitrogen removal problems was considered, and decision trees for diagnosing nitrification and denitrification problems were built and successfully applied to A/O process.

关键词： A/O process nitrification denitrification nitrogen removal decision trees

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nitrogen removal from sludge dewatering effluent through anaerobic ammonia oxidation process

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Journal of Environmental Sciences 2005年第6期17卷 1030-1033页

作者： ZHANG Shao-hui ZHENG Ping HUA Yu-mei Department of Environmental Engineering Zhejiang University Hangzhou 310029 China

Anaerobic ammonia oxidation（Anammox） process is a novel and promising wastewater nitrogen removal process. The feasibility of transition of Anammox from denitrification and the performance of lab-scale Anammox biofilm reactor were investigated with sludge dewatering effluent. The results showed that Anammox process could be successfully started up after cultivation of denitrification biofilm and using it as inoculum. The transition of Anammox from denitrification was accomplished within 85 d. Anammox process was found suitable to remove ammonia from sludge dewatering effluent. The effluent ammonia concentration was detected to be 23.11 mgN/L at HRT of 28 h when influent ammonia concentration was fed 245 mgN/L, which was less than that for the national discharge standard Ⅱ （25 rngN/L） of China. Volumetric total nitrogen loading rate was up to 584.99 mg TN/（L. d） at HRT of 17 h, while influent concentrations were kept 243.25 mg NH4＊ -N/L and 288.31 mg NO2^- -N/L.

关键词： denitrification Anammox sludge dewatering effluent nitrogen removal

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nitrogen removal from Molten Steel under Argon DC Glow Plasma

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Journal of Iron and Steel Research(International) 2005年第4期12卷 6-8页

作者： SUNMing-shan DINGWei-zhong LUXiong-gang TaiyuanIronandSteelGroupCo Taiyuan030003China ShanghaiUniversity Shanghai200072China

Under argon DC glow plasma, the nitrogen removal from molten steel was studied. The experimental result showed that nitrogen mass percent could be reduced to 0000 8%. The change of polarity had no impact on nitrogen removal when the nitrogen mass percent was low. The mechanism of denitrogenation of molten steel under argon DC glow plasma was discussed.

关键词： argon DC glow plasma nitrogen removal molten steel kinetic

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Experimental analysis of a nitrogen removal process simulation of wastewater land treatment under three different wheat planting densities

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Journal of Environmental Sciences 2002年第3期14卷 317-324页

作者： WangHQ ChenJJ StateKeyJointLaboratoryofEnvironmentalSimulationandPollutionControl InstituteofEnvironmentalSciencesBeijinNormalUniversityBeijing100875China.E－mail：whongqi@*** StateKeyJointLaboratory

nitrogen contaminant transport, transformation and uptake simulation experiments were conducted in green house under three different planting density of winter wheat. They were Group A, planting density of 0.0208 plants/cm 2, Group B, 0.1042 plants /cm 2, and Group C, 0.1415 plants/cm 2. The capacity and ratio of nitrogen removal were different on three kinds of conditions of wastewater land treatment. From analysis of wastewater treatment capacity, wastewater concentration and irrigation intensity for Group C were suitable and nitrogen quantity added was 2 times of that for Group B, 2.6 times for Group A while nitrogen residue was only 7.06%. Hence, wastewater irrigation and treatment design with purpose of waste water treatment should select the design with maximum capacity, optimal removal ratio and least residue in soil, which was closely related to crop planting density, crop growth status and also background nitrogen quantity in soil.

关键词： wastewater land treatment planting density winter wheat nitrogen removal soil

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Influence of seasonal temperature change on autotrophic nitrogen removal for mature landfill leachate treatment with high-ammonia by partial nitrification-Anammox process

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Journal of Environmental Sciences 2021年第4期33卷 291-300页

作者： Xiang Li Ming-yu Lu Yong Huang Yi Yuan Yan Yuan School of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology Suzhou 215009China

In this study,a denitrification(DN)–partial nitritation(PN)–anaerobic ammonia oxidation(Anammox)system for the efficient nitrogen removal of mature landfill leachate was built with a zone-partitioning self-reflux biological reactor as the core device,and the effects of changes in seasonal temperature on the nitrogen removal in non-temperature-control environment were explored.The results showed that as the seasonal temperature decreased from 34℃to 11.3℃,the total nitrogen removal rate of the DN-PN-Anammox system gradually decreased from the peak value of 1.42 kg/(m^(3)·day)to 0.49 kg/(m^(3)·day).At low temperatures(nitrogen load(NLR)of the system is not appropriate,the fluctuation of high NH_(4)^(+)-N concentration in the landfill leachate greatly influenced the stability of the nitrogen removal.At temperatures of 11℃–15℃,the NLR of the system is controlled below 0.5 kg/(m^(3)·day),which can achieve stable nitrogen removal and the nitrogen removal efficiency can reach above 96%.The abundance of Candidatus Brocadia gradually increased with the decrease of temperature.Nitrosomonas,Candidatus Brocadia and Candidatus Kuenenia as the main functional microorganisms in the low temperature.

关键词： Landfill leachate Denitrification-partial nitrification-Anammox Seasonal temperature nitrogen removal Microbiology community

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nitrogen and phosphorus removal under intermittent aeration conditions

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Journal of Environmental Sciences 2002年第4期14卷 541-546页

作者： XIA Si\|qing, GAO Ting\|yao, ZHOU Zeng\|yan (State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092, China.) Tongji University State Key Laboratory of Pollution Control and Resource Reuse 上海 200092 Tongji University State Key Laboratory of Pollution Control and Resource Reuse 上海 200092

A practice wastewater treatment plant was operated using intermittent aeration activated sludge process to enhance biological nitrogen and phosphorus removal. When the influent concentrations of COD Cr , BOD 5, TN, TP, NH 3\|N, TKN, and SS varied in a range of 207.5—1640 mg/L, 61.8—637 mg/L, 28.5—75.6 mg/L, 4.38—20.2 mg/L, 13.6—31.9 mg/L, 28.5—75.6 mg/L, and 111—1208 mg/L, the effluent means were less than 50 mg/L, 20 mg/L, 5 mg/L, 1.0 mg/L, 5 mg/L, 10 mg/L, and 20 mg/L, respectively. Based on a long time of operating results, this process is very suitable for nutrient biological removal for treating the municipal wastewater those water characteristics are similar as that of the Songjiang Municipal Waste Water Treatment Plant(SJMWTP).

关键词： intermittent aeration process high concentration activated sludge nitrogen removal phosphorus removal

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