检索结果-南通市图书馆

Proteomic response of Phaeocystis globosa to nitrogen limitation

Journal of Oceanology and Limnology 2024年第1期42卷 141-149页

作者： Haisu LIU Ruiwang WEI Qiangyong LEI Lei CUI Songhui LÜ Key Laboratory of Eutrophication and Red Tide Prevention Research Center for Harmful Algae and Marine BiologyJinan UniversityGuangzhou 510632China Southern Marine Science and Engineering Guangdong Laboratory Zhuhai 519082China National Engineering Laboratory of Electronic and Information Standardization Shenzhen 518057China

Phaeocystis globosa is an important unicellular eukaryotic alga that can also form colonies.P.globosa can cause massive harmful algal blooms and plays an important role in the global carbon or sulfur cycling.Thus far,the ecophysiology of P.globosa has been investigated by numerous studies.However,the proteomic response of P.globosa to nitrogen depletion remains largely unknown.We compared four protein preparation methods of P.globosa for two-dimensional electrophoresis(2-DE)(Urea/Triton X-100 with trichloroacetic acid(TCA)/acetone precipitation;TCA/acetone precipitation;Radio Immuno Precipitation Assay(RIPA)with TCA/acetone precipitation;and Tris buffer).Results show that the combination of RIPA with TCA/acetone precipitation had a clear gel background and showed the best protein spot separation effect,based on which the proteomic response to nitrogen depletion was studied using 2-DE.In addition,we identified six differentially expressed proteins whose relative abundance increased or decreased more than 1.5-fold(Pnitrogen limitation,replication protein-like,RNA ligase,and sn-glycerol-3-phosphate dehydrogenase were reduced,which may decrease the DNA replication level and ATP production in P.globosa cells.The increase of endonucleaseⅢand transcriptional regulator enzyme may affect the metabolic and antioxidant function of P.globosa cells and induce cell apoptosis.These findings provide a basis for further proteomic study of P.globosa and the optimization of protein preparation methods of marine microalgae.

关键词： Phaeocystis globosa nitrogen limitation proteomic response two-dimensional electrophoresis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparing the temperature sensitivity of organic matter decomposition in oxic and oxygen-deprived soils

引用

Soil Ecology Letters 2024年第1期6卷 29-32页

作者： Zhenhui Jiang Xin Wang Ting Liu Xiaojuan Feng State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeiing 100093China University of Chinese Academy of Sciences Bejing 100049China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China Henan Key Laboratory of Ecological Environment Protection and Restoration of Yellow River Basin Yellow River Institute of Hydraulic ResearchZhengzhou 450003China

No consistent variation was found in soil respiration Q10 under various O2 conditions.Substrate C quality had a strong effect on Q10 in oxic soils.N limitation had a large impact on Q10 in soils under O2 limitation.Current studies on the temperature sensitivity(Q10)of soil organic matter(SOM)decomposition mainly focus on aerobic conditions.However,varia-tions and determinants of Q10 in oxygen(O2)-deprived soils remain unclear.Here we incubated three grassland soils under oxic,suboxic,and anoxic conditions subjected to varying temperatures to compare variations in Q10 in relation to changing substrates.No consistent variation was found in Q10 under various O2 conditions.Further analysis of edaphic properties demon-strated that substrate carbon quality showed a strong influence on Q10 in oxic soils,whereas nitrogen limitation played a more important role in suboxic and anoxic soils.These results suggest that substrate carbon quality and nitrogen limitation may play roles of varying importance in determining the temperature sensitivity of SOM decomposition under various O2 conditions.

关键词： oxygen-limited conditions temperature sensitivity soil respiration carbon substrate nitrogen limitation

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effects of phosphorus and nitrogen limitation on PHA production in activated sludge

引用

Journal of Environmental Sciences 2010年第10期22卷 1602-1607页

作者： Qinxue Wen,Zhiqiang Chen,Ting Tian,Wei Chen State Key Laboratory of Urban Water Resource and Environment,Harbin Institute of Technology,Harbin 150090,China State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Harbin 150090 China

The effects of phosphorus and nitrogen limitation on polyhydroxyalkanoate （PHA） production and accumulation by activated sludge biomass with acetate as a carbon source were investigated. Pre-selected influent carbon-phosphorus （C：P, W/W） of 100, 160, 250, 500 and 750, and carbon-nitrogen （C：N, W/W） of 20, 60, 100, 125 and 180 were applied in the phosphorus limitation experiments and the nitrogen limitation experiments, respectively. The maximum PHA accumulation up to 59% of the cell dry weight with a PHA productivity of 1.61 mg PHA/mg COD consumed was observed at the C：N 125 in the nitrogen limitation experiment. This value was much higher than that obtained in previous studies with a normal substrate feeding. The study showed that activated sludge biomass would produce more polyhydroxybutyrate than polyhydroxyvalerate under the stress of nutrient limitation, especially under phosphorus limitation conditions. The experimental result also indicated that both phosphorus and nitrogen limitation may cause sludge bulking.

关键词： polyhydroxyalkanoate acetate nitrogen limitation phosphorus limitation sludge bulking

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Dynamic carbon-nitrogen coupling under global change

引用

Science China(Life Sciences) 2023年第4期66卷 771-782页

作者： Shuli Niu Lei Song Jinsong Wang Yiqi Luo Guirui Yu Key Laboratory of Ecosystem Network Observation and Modelling Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China Center for Ecosystem Science and Society Department of Biological SciencesNorthern Arizona UniversityFlagstaffAZ 86011USA

Carbon-nitrogen coupling is a fundamental principle in ecosystem ecology.However,how the coupling responds to global change has not yet been examined.Through a comprehensive and systematic literature review,we assessed how the dynamics of carbon processes change with increasing nitrogen input and how nitrogen processes change with increasing carbon input under global change.Our review shows that nitrogen input to the ecosystem mostly stimulates plant primary productivity but inconsistently decreases microbial activities or increases soil carbon sequestration,with nitrogen leaching and nitrogenous gas emission rapidly increasing.nitrogen fixation increases and nitrogen leaching decreases to improve soil nitrogen availability and support plant growth and ecosystem carbon sequestration under elevated CO_(2)and temperature or along ecosystem succession.We conclude that soil nitrogen cycle processes continually adjust to change in response to either overload under nitrogen addition or deficiency under CO_(2)enrichment and ecosystem succession to couple with carbon cycling.Indeed,processes of both carbon and nitrogen cycles continually adjust under global change,leading to dynamic coupling in carbon and nitrogen cycles.The dynamic coupling framework reconciles previous debates on the“uncoupling”or“decoupling”of ecosystem carbon and nitrogen cycles under global change.Ecosystem models failing to simulate these dynamic adjustments cannot simulate carbonnitrogen coupling nor predict ecosystem carbon sequestration well.

关键词： nitrogen limitation carbon-nitrogen interaction global change carbon sequestration soil nitrogen cycle

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

How do nitrogen-limited alpine coniferous forests acquire nitrogen?A rhizosphere perspective

引用

Forest Ecosystems 2022年第6期9卷 745-754页

作者： Huajun Yin Bartosz Adamczyk Qitong Wang Biao Zhu Wanji Guo Xiaomin Zhu Qing Liu Ziliang Zhang CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu610041China China-Croatia“Belt and Road”Joint Laboratory on Biodiversity and Ecosystem Services Chengdu Institute of BiologyChinese Academy of SciencesChengdu610041China Natural Resources Institute Finland PL 200791HelsinkiFinland Institute of Ecology College of Urban and Environmental Sciencesand Key Laboratory for Earth Surface Processes of the Ministry of EducationPeking UniversityBeijing100871China Institute for Sustainability Energyand EnvironmentUniversity of Illinois at Urbana-ChampaignUrbanaIL61801USA

Background:Alpine coniferous forest ecosystems dominated by ectomycorrhizal(ECM)tree species are generally characterized by low soil nitrogen(N)availability but stabilized plant productivity.Thus,elucidating potential mechanisms by which plants maintain efficient N acquisition is crucial for formulating optimized management practices in these ecosystems.Methods:We summarize empirical studies conducted at a long-term field monitoring station in the alpine coniferous forests on the eastern Tibetan Plateau,China.We propose a root-soil interaction-based framework encompassing key components including soil N supply,microbial N transformation,and root N uptake in the rhizosphere.Results:We highlight that,(i)a considerable size of soil dissolved organic N pool mitigates plant dependence on inorganic N supply;(ii)ectomycorrhizal roots regulate soil N transformations through both rhizosphere and hyphosphere effects,providing a driving force for scavenging soil N;(iii)a complementary pattern of plant uptake of different soil N forms via root-and mycorrhizal mycelium-pathways enables efficient N acquisitions in response to changing soil N availability.Conclusions:Multiple rhizosphere processes abovementioned collaboratively contribute to efficient plant N acquisition in alpine coniferous forests.Finally,we identify several research outlooks and directions to improve the understanding and prediction of ecosystem functions in alpine coniferous forests under on-going global changes.

关键词： Plant nitrogen acquisition Root-soil interaction Alpine coniferous forests nitrogen limitation Rhizosphere

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：