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中国海洋湖沼学会第十次全国会员代表大会暨学术研讨会

作者： Hong Du Yang Wang Xuejiao Bei Mingqi Zhong Department of Biology Shantou University

nitrogen significantly influence algae productivity and acquirement in ocean ecosystems. Macroalgae, limited by the nitrogen nutrients varying with seasonal oscillations and geographic settings,play a significant role in nitrogen utilization and storage. nitrogen storage mainly includes free amino acids, soluble proteins and photosynthetic pigments in algae. However, endocellular nitrogen storage may be depleted after a long period growth of the algae in lack of nitrogen condition. We cultured algae in N deprivation condition （-N treatment） for twelve days comparing to algae in N repletion condition （+N treatment）. This study aimed at explaining the response of macroalgae nitrogen and carbon metabolism to abrupt N deprivation condition at physiological and proteomic levels. Our data supported that the major storage units rapidly decreased and glutamine synthetase activity （GSA） reduced sharply to extremely low level during next 10 days after increasing by 33.69% in first two days under N deprived condition, but chlorophyll-a showed not significant differences from control group in this study （P＞0.05）. Furthermore, we proved that N-storage capacity was approximately 50% of total N in G. lemaneiformis by stoichiometry math model. The N deprivation group compared with the N repletion group at proteomic level on day two, four, eight and twelve. 101 protein spots were distinguishable in total using two-dimensional gel electrophoresis including 54 proteins increasing and 47 decreasing in abundance （greater than 1.5-fold change）, but we just identified 36 spots in 101 protein spots by MS. The proteins’ names are based on NCBI and the categories of proteins are based on AmiGO. There are four categories of proteins mainly involved in the response to nitrogen deprivation: nitrogen metabolism, carbohydrate metabolism, photosynthesis and energy metabolism and translational and transcriptional regulation.

关键词： Gracilaria lemaneiformis nitrogen deprivation storage stoichiometry 2DE

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PuHox52 promotes coordinated uptake of nitrate,phosphate, and iron under nitrogen deficiency in Populus ussuriensis

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Journal of Integrative Plant Biology 2023年第3期65卷 791-809页

作者： Ming Wei Mengqiu Zhang Jiali Sun Ying Zhao Solme Pak Miaomiao Ma Yingxi Chen Han Lu Jingli Yang Hairong Wei Yuhua Li Chenghao Li State Key Laboratory of Tree Genetics and Breeding School of ForestryNortheast Forestry UniversityHarbin 150040China College of Life Sciences Northeast Forestry UniversityHarbin 150040China College of Forest Resources and Environmental Science Michigan Technological UniversityHoughtonMichigan 49931USA Key Laboratory of Saline-alkali Vegetation Ecology Restoration(Northeast Forestry University) Ministry of EducationHarbin 150040China

It is of great importance to better understand how trees regulate nitrogen(N) uptake under N deficiency conditions which severely challenge afforestation practices, yet the underlying molecular mechanisms have not been well elucidated. Here,we functionally characterized PuHox52, a Populus ussuriensis HD-ZIP transcription factor, whose overexpression greatly enhanced nutrient uptake and plant growth under N deficiency. We first conducted an RNA sequencing experiment to obtain root transcriptome using PuHox52-overexpression lines of P. ussuriensis under low N treatment. We then performed multiple genetic and phenotypic analyses to identify key target genes of PuHox52 and validated how they acted against N deficiency under PuHox52 ***52 was specifically induced in roots by N deficiency, and overexpression of PuHox52promoted N uptake, plant growth, and root development. We demonstrated that several nitrate-responsive genes(PuNRT1.1, PuNRT2.4,PuCLC-b, PuNIA2, PuNIR1, and PuNLP1),phosphate-responsive genes(PuPHL1A and PuPHL1B), and an iron transporter gene(PuIRT1) were substantiated to be direct targets of PuHox52. Among them, PuNRT1.1, PuPHL1A/B, and PuIRT1 were upregulated to relatively higher levels during PuHox52-mediated responses against N deficiency in PuHox52-overexpression lines compared to WT. Our study revealed a novel regulatory mechanism underlying root adaption to N deficiency where PuHox52 modulated a coordinated uptake of nitrate, phosphate, and iron through 'PuHox52-PuNRT1.1', 'PuHox52-PuPHL1A/PuPHL1B', and'PuHox52-PuIRT1' regulatory relationships in poplar roots.

关键词： HD-Zip transcription factor iron transport nitrate transport nitrogen deprivation phosphate absorption Populus ussuriensis root architecture target gene

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Expression analyses of miRNA Up-MIR-843 and its target genes in Ulva prolifera

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Acta Oceanologica Sinica 2020年第10期39卷 27-34页

作者： Juanjuan Yang Dachun Yu Songdong Shen Department of Cell Biology School of Biology and Basic MedicalSoochow UniversitySuzhou 215123China

microRNAs(miRNA)families play a critical role in plant growth,development,and responses to abiotic *** this study,we characterized Up-miR-843 and its targets genes in Ulva prolifera responses to nitrogen depravation and heat *** data demonstrated that 184 target genes of Up-miR-843 could be successfully validated.N deficiency not heat stress stimulus induced increase in abundance of the Up-miR-843 while exhibited reverse expression of target genes,including cyclin A3 and cyclin L,which were strictly required for cell cycle *** addition,*** with highly expression of Up-miR-843 showed improved biomass,and photosynthesis compared with that under normal growth ***,the N deprivation and heat responsive miRNAs might be a possible member mediating the expression of these target genes,which further regulated the growth of ***.

关键词： cyclins nitrogen deprivation microRNA U.prolifera heat stress

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Involvement of phosphatidate phosphatase in the biosynthesis of triacylglycerols in Chlamydomonas reinhardtii

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1121-1131页

作者： Xiao-dong DENG Jia-jia CAI Xiao-wen FEI Key Laboratory of Tropical Crop Biotechnology Ministry of Agriculture Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences School of Science Hainan Medical College

Lipid biosynthesis is essential for eukaryotic cells, but the mechanisms of the process in microalgae remain poorly understood. Phosphatidic acid phosphohydrolase or 3-sn-phosphatidate phosphohydrolase(PAP) catalyzes the dephosphorylation of phosphatidic acid to form diacylglycerols and inorganic orthophosphates. This reaction is integral in the synthesis of triacylglycerols. In this study, the mRNA level of the PAP isoform CrPAP2 in a species of Chlamydomonas was found to increase in nitrogen-free conditions. Silencing of the CrPAP2 gene using RNA interference resulted in the decline of lipid content by 2.4%–17.4%. By contrast, over-expression of the CrPAP2 gene resulted in an increase in lipid content by 7.5%–21.8%. These observations indicate that regulation of the CrPAP2 gene can control the lipid content of the algal cells. In vitro CrPAP2 enzyme activity assay indicated that the cloned CrPAP2 gene exhibited biological activities.

关键词： Phosphatidate phosphohydrolase 2 Triacylglycerol biosynthesis RNAi Chlamydomonas reinhardtii nitrogen deprivation Over-expression

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