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The Crop Journal 2022年第6期10卷 1623-1632页

作者： Xinxin Zhu Dujun Wang Lijuan Xie Tao Zhou Jingyi Zhao Qian Zhang Meng Yang Wenjuan Wu Xingming Lian National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhan 430070HubeiChina

nitrate(NO_(3)^(-)) uptake involves a finely regulated and complex multilevel response *** the molecular mechanism of nitrate uptake may lead to improving the growth and productivity of plants in the presence of dynamic variation in nitrate *** this study,we identified three lateral organ boundaries domain(LBD)transcription factors,OsLBD37,OsLBD38,and OsLBD39,as regulators of nitrate uptake in response to nitrogen(N)***37,OsLBD38,and OsLBD39 were induced by ammonium and glutamine in rice *** or collective knockout of OsLBD37,OsLBD38,and OsLBD39 led to increased concentrations of nitrate and increased expression of OsNRT2.1,OsNRT2.2,and OsNRT2.3respectively under high-N conditions,whereas overexpression of each of these three LBD genes produced opposite effects where N accumulation was ***-luciferase reporter assay further confirmed that OsLBD37,OsLBD38,and OsLBD39 possessed transcription inhibitory activities in rice protoplast cells,downregulating the expression of OsNRT2.1/OsNRT2.2/*** two-hybrid and bimolecular fluorescence complementation assays showed that OsLBD37 interacted with OsLBD37,OsLBD38,and OsLBD39in the ***,these results show that OsLBD37,OsLBD38,and OsLBD39 collaborate to inhibit the expression of OsNRT2.1/OsNRT2.2/OsNRT2.3 transporters under N-sufficient conditions,thereby helping rice plants avoid excessive nitrate accumulation that may affect their growth.

关键词： Oslbd37/Oslbd38/Oslbd39 Transcription factor nitrate uptake Rice

Ammonium Effects on nitrate uptake by Roots of Upland and Paddy Rice Seedlings Related to Membrane Potential Differences

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Agricultural Sciences in China 2010年第6期9卷 799-805页

作者： WANG Xiao-li WANG Yu-qian TAO Yue-yue FENG Ke College of Environmental Science and Engineering Yangzhou University Yangzhou 225009 P.R. China

nitrate uptake characteristics and ammonium effects on nitrate uptake were compared between upland rice （Brazilian upland rice） and paddy rice （Wuyujing 3 and Yangdao 6） through the glass microelectrode technique and the concentration gradient method of uptake *** indicated that nitrate uptake by rice seedlings and ammonium effects were depending on membrane potential of root *** upland rice and paddy rice presented obviously different *** all cultivars,the nitrate treatments induced rapid depolarization and then slow repolarization of membrane potential in root epidermal cells,and even hyperpolarization was observed when nitrate concentration was *** membrane potential of epidermal cells in Brazilian upland rice roots was larger and its response to NO3- was bigger than those of two paddy rice *** of membrane potential was amplified when ammonium was simultaneously added with nitrate into the measure medium,but repolarization was reduced,even *** upland rice seedlings had high Vmax of nitrate uptake and low Km,furthermore,Vmax and Km were little affected by ammonium,but Vmax of Wuyujing 3 was reduced ***,inhibition of NH4＋ differed obviously between upland rice and paddy rice.

关键词： nitrate uptake membrane potential ammonium upland rice paddy rice

nitrate uptake of Rice as Affected by Growth Stages and Ammonium

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Agricultural Sciences in China 2003年第1期2卷 62-67页

作者： FENG Ke, WANG Xiao-li, CHEN Ping and SHENG Hai-junAgricultural College , Yangzhou University , Yangzhou 225009 , P. R. China Agricultural College Yangzhou University Yangzhou 225009 P. R. China

Solution culture experiments were carried out to study the NO3- uptake kinetics by rice roots and the nitrate reductase activity (NRA) in leaves of four typical rice genotypes (conventional indica, conventional japonica, hybrid indica, and hybrid japonica) at different growth stages. The effects of NH4+ on the NO3- uptake kinetics was also studied. The results indicated that the four genotypes responded differently to NO3- and NH4+. Comparing indica rice with japonica rice, hybrid rice with conventional rice, formers absorbed NO3-more rapidly than laters. The superiority of indica rice and hybid rice for NO3- uptake was mainly due to the higher Vmax at the early growth stage (20 days) and the higher affinity of nitrate transporters for NO3- at the middle growth stage (50 days). At both growth stages NH4+ significantly inhibited NO3- uptake for japonica rice, but showed little effect for indica rice. Conversely, NH4+ stimulated the uptake of NO3- for hybrid rice. NH4+ could repress NRA in leaves of all four genotypes with a sensitive order as following: hybrid japonica > hybrid indica > conventional indica > conventional japonica.

关键词： Rice genotype nitrate uptake nitrate reductase Ammonium

Physiological and Molecular Response of Wheat Roots to nitrate Supply in Seedling Stage

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Agricultural Sciences in China 2011年第5期10卷 695-704页

作者： WANG Ping WANG Zhen-lin CAI Rui-guo LI Yong CHEN Xiao-guang YIN Yan-ping State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/Agronomy College Shandong Agricultural University Tai＇an 271018 P.R.China Agronomy Department Hebei Normal University of Science and Technology Changli 066600 P.R. China

The objective of this study was to understand the morphological,physiological,and molecular responses of wheat roots to nitrate supply at seedling *** wheat genotypes,Jimai 22 and Shannong 15,were grown in Hoagland＇s nutrient solution with different nitrate levels at seedling *** indicated that the plant dry weight and N accumulation increased with the increase of nitrate *** number of axial root,total uptake area （TUA）,and active uptake area （AUA） increased with more nitrate *** analysis indicated that significant positive correlations existed between N accumulation and dry weight,N accumulation and AUA,and N accumulation and AUA/***,the expressions of NRT2.1,NRT2.2,and NRT2.3 decreased with nitrate supply increased,the expressions of NRT1,NRT2.1,and NRT2.3 could maintain high level at N3 *** free amino acid and NO3- content in shoot also increased with the increased nitrate application,but no significant difference was found in root among the *** results implied that the increase of N uptake by nitrate supply was due to the morphological and physiological responses of wheat roots and the high expression level of TaNRT ***,the contribution of morphological,physiological,and molecular parameters was different between two genotypes of wheat.

关键词： wheat root parameters nitrate uptake N metabolites TaNRT expression

A comparison of nitrate transport in four different rice (Oryza sativa L.) cultivars

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Science China Life Sciences 2005年第S2期48卷 897-911页

作者： Anthony J.Miller Crop Performance and Improvement Division Rothamsted Research Harpenden U.K. Hertfordshire AL5 2JQ

As rice can use both nitrate (NO3-) and ammonium (NH4+), we have tested the hypothesis that the shift in the pattern of cultivars grown in Jiangsu Province reflects the ability of the plants to exploit NO3- as a nitrogen (N) source. Four rice cultivars were grown in solution culture for comparison of their growth on NO3- and NH4+ nitrogen sources. All four types of rice, Xian You 63 (XY63), Yang Dao 6 (YD), Nong Keng 57 (NK) and Si You 917 (SY917), grew well and produced similar amounts of shoot biomass with 1 mmol/L NH4+ as the only N source. However, the roots of NK were significantly smaller in comparison with the other cultivars. When supplied with 1 mmol/L NO3- YD produced the greatest biomass; while NK achieved the lowest growth among the four cultivars. Electrophysiological measurements on root rhizodermal cells showed that the NO3- elicited changes in membrane potential (△Em) of these four rice cultivars were significantly different when exposed to low external NO3- (uptake depletion kinetics were also compared and for some cultivars (YD and XY63) a single-phase uptake system with first order kinetics best fitted the data; while other cultivars (ND and SY917) showed a better fit to two uptake systems. These uptake systems had two affinity ranges: one had a similar Km in all the cultivars (0.2 mmol/L); the other much higher affinity system (0.03 mmol/L) was only present in NK and SY917. The expression pattern of twelve different NO3-

关键词： Oryza saliva rice cultivars nitrate uptake nitrate transporters.

Root Morphology, Plant Growth, nitrate Accumulation and Nitrogen Metabolism of Temperate Lettuce Grown in the Tropics with Elevated Root-Zone CO₂ at Different Root-Zone Temperatures

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American Journal of Plant Sciences 2016年第14期7卷 1821-1833页

作者： Jie He Lin Qin Sing Kong Lee Natural Sciences and Science Education Academic Group National Institute of Education Nanyang Technological University Singapore

This paper investigated the effects of root-zone (RZ) CO2 concentration ([CO2]) on root morphology and growth, nitrate (NO3-) uptake and assimilation of lettuce plants at different root-zone temperatures (RZT). Elevated RZ [CO2] stimulated root development, root and shoot growth compared to ambient RZ [CO2]. The greatest increase in root growth was observed in plants grown under elevated RZ [CO2] of 50,000 ppm. However, RZ [CO2] of 10,000 ppm was sufficient to achieve the maximal leaf area and shoot productivity. Lettuce plants exhibited faster shoot and root growth at 20°C-RZT than at ambient (A)-RZT. However, under elevated RZ [CO2], the magnitude of increased growth was greater at A-RZT than at 20°C-RZT. Compared to RZ [CO2] of 360 ppm, elevated RZ [CO2] of 10,000 ppm increased NO3- accumulation and nitrate reductase activity (NRA) in both leaves and roots. NO3- concentrations of leaf and root were higher at 20°C-RZT than at A-RZT in all plants. NRA was higher in root than in leaf especially under A-RZT. The total reduced nitrogen (TRN) concentration was significantly higher in plants grown under elevated RZ [CO2] of 10,000 ppm than under ambient RZ [CO2] of 360 ppm with greater concentration in 20°C-RZT plants than in A-RZT plants. These results imply that elevated RZ [CO2] significantly affected root morphology, root and shoot growth and N metabolism of temperate lettuce with greater impacts at A-RZT than at 20°C-RZT. These findings have practical significance to vegetable production by growing the vegetable crops at cool-RZT with elevated RZ [CO2] to enhance its productivity.

关键词： Lettuce nitrate Assimilation nitrate uptake Root Morphology Root-Zone CO2 Root-Zone Temperature

Engineering nitrogen use efficiency in wheat

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Engineering nitrogen use efficiency in wheat