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Functional Characterization of a Putative nitrate transporter Gene Promoter from Rice

Acta Biochimica et Biophysica Sinica 2006年第11期38卷 795-802页

作者： Ting-Zhang HU~(1,3) Kai-Ming CAO~1 Mian XIA~2 Xi-Ping WANG~(1,2*) 1 Department of Biochemistry,School of Life Science Fudan University,Shanghai 200433,China 2 National Center for Molecular Crop Design,Weiming Kaituo Agriculture Biotech Co.,Ltd.,Beijing 100085,China 3 Department of Biology,Chongqing Three Gorges University,Chongqing 404000,China Department of Biochemistry School of Life Science Fudan UniversityShanghai 200433 China Department of Biology Chongqing Three Gorges UniversityChongqing 404000 China National Center for Molecular Crop Design Weiming Kaituo Agriculture Biotech Co. Ltd.Beijing 100085 China

Drought is one of the most significant abiotic stresses that influence plant growth *** analysis revealed that OsNRT1.3,a putative nitrate transporter gene in rice,wasinduced by *** confirm if the OsNRT1.3 promoter can respond to drought stress,a 2019 bpupstream sequence of OsNRT1.3 was *** OsNRT1.3 promoter fragments were generated by5′-deletion,and fused to the β-glucuronidase (GUS) *** chimeric genes were introduced into ***2019::GUS,NRT1196::GUS and NRT719::GUS showed similar expression patterns in seeds,roots,leaves and flowers in all transgenic rice,and GUS activity conferred by different OsNRT1.3 promoterfragments was significantly upregulated by drought stress,indicating that OsNRT1.3 promoter responds todrought stress and the 719 bp upstream sequence of OsNRT1.3 contains the drought response elements.

关键词： deletion analysis drought nitrate transporter OsNRT1.3 promoter

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OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield

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The Crop Journal 2022年第2期10卷 397-406页

作者： Jie Wang Renjing Wan Haipeng Nie Shaowu Xue Zhongming Fang Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education) College of Agricultural SciencesGuizhou UniversityGuiyang 550025GuizhouChina Center of Applied Biotechnology Wuhan University of BioengineeringWuhan 430415HubeiChina College of Life Science and Technology Huazhong Agricultural UniversityWuhan 430070HubeiChina

Rice has a large number of nitrate or peptide transporter family(NPF) genes, but the effects of most members on rice growth and development are unknown. We report that Os NPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of Os NPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica *** NPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In c RNA-injected Xenopus laevis oocytes, Os NPF5.16 transport of nitrate at high nitrate concentration depended on p H. Overexpression of Os NPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of Os NPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of Os NPF5.16 expression showed the opposite effects. Regulating Os NPF5.16 expression has potential for improving rice grain yield.

关键词： Rice OsNPF5.16 nitrate transporter Natural variation Growth Grain yield

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Overexpression of a pH-sensing nitrate transporter in the membrane to increase rice crop yields

Overexpression of a pH-sensing nitrate transporter in the me...

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2016年全国植物生物学大会

作者： Xiaorong Fan Zhong Tang Yawen Tan Yong Zhang Bingbing Luo Meng Yang Xingming Lian Qirong Shen Anthony J.Miller Guohua Xu State Key Laboratory of Crop Genetics and Germplasm Enhancement Key Laboratory of Plant Nutrition and Fertilization in Lower-Middle Reaches of the Yangtze River Ministry of AgricultureNanjing Agricultural University Metabolic Biology Department John Innes CentreNorwich Research Park National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University

Cellular pH homeostasis is fundamental for life and all cells adapt to maintain this balance. In plants the chemical form of nitrogen supply,nitrate and ammonium,is one of the cellular p H dominators. We report that the rice nitrate transporter OsNRT2.3 is transcribed into two spliced isoforms with natural variation in their expression ratio. One splice form,OsNRT2.3b is plasma membrane located mainly expressed in the phloem,and which has a regulatory motif on the cytosolic side that acts to switch nitrate transport activity on or off by a p H sensing mechanism. High OsNRT2.3b expression in rice enhances the p H buffering capacity of the plant,increasing N,Fe and P uptake. I n field trials,increased expression of OsNRT2.3b improved grain yield and nitrogen use efficiency(NUE) by 40%. These results indicate that p H sensing by the rice nitrate transporter OsNRT2.3b is important for plant adaption to varied N supply forms and can provide a target of improving NUE.

关键词： nitrate transporter p H-sensing nitrogen use efficiency yield rice

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