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Journal of Oceanology and Limnology 2021年第1期39卷 362-371页

作者： Zhihai ZHONG Zhengyi LIU Longchuan ZHUANG Wanlin SONG Weizhou CHEN Yantai Institute of Coastal Zone Research Chinese Academy of SciencesYantai 264003China Shantou University Shantou 515063China

Gracilariopsis lemaneiformis is an economically-valued species and widely cultured in China at *** being acclimated to diff erent growth temperatures(15,20,25,and 30°C)for 7 days,the relative growth rate(RGR),nitrate reductase activity,soluble protein content and chlorophyll a fl uorescence of *** were *** show that RGR was markedly aff ected by temperature especially at 20°C at which *** exhibited the highest eff ective quantum yield of PSII[Y(II)]and lightsaturated electron transport rate(ETR max),but the lowest non-photochemical *** of growth temperature,the nitrate reductase activity increased with the incubation temperature from 15 to 30°*** addition,the greatest nitrate reductase activity was found in the thalli grown at 20°*** value of temperature coeffi cient Q10 of alga cultured in 15°C was the greatest among those of other temperatures *** indicate that the optimum temperature for nitrate reductase synthesis was relatively lower than that for nitrate reductase activity,and the relationship among growth,photosynthesis,and nitrate reductase activity showed that the optimum temperature for activity of nitrate reductase in vivo assay should be the same to the optimal growth temperature.

关键词： chlorophyll a fl uorescence Gracilariopsis lemaneiformis growth nitrate reductase activity

nitrate reductase activity and its diurnal variation rhythm for Camptotheca acuminata seedlings

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Journal of Forestry Research 2004年第3期15卷 167-170页

作者：孙世芹阎秀峰东北林业大学

nitrate reductase activity (NRA) in different plant organs and leaves in different positions of Camptotheca acuminata seedlings was determined by an In vivo assay, the diurnal variation rhythm of NRA in leaves of different positions was observed,and the correlations between leaf NRA, leaf area and lamina mass per unit area (LMA) were also examined. The results showed that NRA in the leaf was significantly highest, compared with that in other organs such as roots, stems and leaves. In this experiment, the 10 leaves were selected from the apex to the base of the seedlings in order. The different NRA occurred obviously in leaves of different positions of C. acuminata seedlings from the apex to the base, and NRA was higher in the 4th-6th *** diurnal change rhythm of leaf NRA showed a one peak curve, and maximum NRA value appeared at about midday (at 12:30 or so). No obvious correlations between NRA and leaf area or lamina mass per unit area were observed. This study offered scientific foundation for the further research on nitrogen metabolism of C. acuminata.

关键词： Camptotheca acuminata nitrate reductase activity Diurnal variation

The δ^15N response and nitrate assimilation of Orychophragmus violaceus and Brassica napus plantlets in vitro during the multiplication stage cultured under different nitrate concentrations

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Acta Geochimica 2017年第2期36卷 190-197页

作者： Kaiyan Zhang Yanyou Wu State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 550081 China University of Chinese Academy of Sciences Beijing 100049 China

Natural nitrogen isotope composition(δ^(15)N) is an indicator of nitrogen sources and is useful in the investigation of nitrogen cycling in organisms and ecosystems. δ^(15)N is also used to study assimilation of inorganic nitrogen. However, the foliar δ^(15)N of intact plants, which is a consequence of nitrate assimilation occurring in the roots and shoots, is not suited for studying nitrate assimilation in cases where nitrate is the sole nitrogen source. In this study, Orychophragmus violaceus(Ov) and Brassica napus(Bn) plantlets, in which nitrate assimilation occurred in the leaves, were used to study the relationship between foliar δ^(15)N and nitrate *** plantlets were grown in vitro in culture media with different nitrate concentrations, and no root formation occurred for the plantlets during the multiplication *** isotope fractionation occurred in both the Ov and the Bn plantlets under all treatments. Furthermore, the foliar nitrogen content of both the Ov and Bn plantlets increased with increasing nitrate concentration. Foliar nitrogen isotope fractionation was negatively correlated with foliar nitrogen content for both the Ov and Bn plantlets. Our results suggest that the foliar nitrogen isotope fractionation value could be employed to evaluate nitrate assimilation ability and leaf nitrate reductase ***, high external nitrate concentrations couldcontribute to improved foliar nitrogen content and enhanced nitrate assimilation ability.

关键词： δ^15N nitrate assimilation Nitrogen isotopefractionation Nitrogen content nitrate reductase activity

Effect of Nitrogen Starvation on the Responses of Two Rice Cultivars to nitrate Uptake and Utilization

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Pedosphere 2014年第5期24卷 690-698页

作者： YIN Xiao-Ming LUO Wei WANG Song-Wei SHEN Qi-Rong LONG Xiao-Hua College of Resources and Environmental Sciences Nanjing Agricultural University

Ammonium(NH+4) is the main nitrogen(N) form for rice crops, while NH+4near the root surface can be oxidized to nitrate(NO-3)by NH+4-oxidizing bacteria. nitrate can be accumulated within rice tissues and reused when N supply is insufficient. We compared the remobilization of NO-3stored in the tissue and vacuolar between two rice(Oryza sativa L.) cultivars, Yangdao 6(YD6, indica)with a high N use efficiency(NUE) and Wuyujing 3(WYJ3, japonica) with a low NUE and measured the uptake of NO-3, expression of nitrate reductase(NR), NO-3transporter genes(NRTs), and NR activity after 4 d of N starvation following 7-d cultivation in a solution containing 2.86 mmol L-1NO-3. The results showed that both tissue NO-3concentration and vacuolar NO-3activity were higher in YD6 than WYJ3 under N starvation. YD6 showed a 2- to 3-fold higher expression of OsNRT2.1 in roots on the 1st and 4th day of N starvation and had significantly higher values of NO-3uptake(maximum uptake velocity, Vmax) than the cultivar ***, YD6 had significantly higher leaf and root maximum NR activity(NRAmax) and actual NR activity(NRAact) as well as stronger root expression of the two NR genes after the 1st day of N starvation. There were no significant differences in NRAmax and NRAact between the two rice cultivars on the 4th day of N starvation. The results suggested that YD6 had stronger NRA under N starvation, which might result in better NO-3re-utilization from the vacuole, and higher capacity for NO-3uptake and use, potentially explaining the higher NUE of YD6 compared with WYJ3.

关键词： nitrate reductase activity nitrate transpoters nitrogen use efficiency Oryza sativa L. remobilization

Effect of nitrate on Root Development and Nitrogen Uptake of Suaeda physophora Under NaCl Salinity

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Pedosphere 2010年第4期20卷 536-544页

作者： YUAN Jun-Feng FENG Gu MA Hai-Yan TIAN Chang-Yan Key Laboratory of Oasis Ecology and Desert Environment Xinjiang Institute of Ecology and Geography ChineseAcademy of Sciences Urumqi 830011 （China） Graduate-UniversitY of Chinese Academy of Sciences Beijing 100039 （China） College of Resources and Environmental Sciences China Agricultural University Beijing 100094 （China）

The effects of NaCl salinity and NO^-3 on growth, root morphology, and nitrogen uptake of a halophyte Suaeda physophora were evaluated in a factorial experiment with four concentrations of NaCl （1, 150, 300, and 450 mmol L^-1） and three NO^-3 levels （0.05, 5, and 10 mmol L^-1） in solution culture for 30 d. Addition of NO^-3 at 10 mmol L^-1 significantly improved the shoot （P 〈 0.001） and root （P 〈 0.001） growth and the promotive effect of NO^-3 was more pronounced on root dry weight despite the high NaCl concentration in the culture solution, leading to a significant increase in the root：shoot ratio （P 〈 0.01）. Lateral root length, but not primary root length, considerably increased with increasing NaCl salinity and NO^-3 levels （P 〈 0.001）, implying that Na^＋ and NO3^- in the culture solution simultaneously stimulated lateral root growth. Concentrations of Na^＋ in plant tissues were also significantly increased by higher NaCl treatments （P 〈 0.001）. At 10 mmol L^-1 NO^-3, the concentrations of NO^-3 and total nitrogen and nitrate reductase activities in the roots were remarkably reduced by increasing salinity （P 〈 0.001）, but were unaffected in the shoots. The results indicated that the fine lateral root development and effective nitrogen uptake of the shoots might contribute to high salt tolerance of S. physophora under adequate NO^-3 supply.

关键词： halophyte lateral roots nitrate reductase activity root morphology

Promotive effect of pyridine on indole degradation by activated sludge under anoxic conditions

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Frontiers of Environmental Science & Engineering 2007年第4期1卷 493-497页

作者： LI Yongmei LI Wenshu GU Guowei State Key Laboratory of Pollutant Control and Resources Reuse Tongji UniversityShanghai 200092China Jiangsu Electric Power Design Institute Nanjing 210024China

Batch experiments were carried out to investigate the promotive effect of pyridine on indole degradation under denitrifying *** seed sludge was obtained from a local coal-coking wastewater treatment facility and was acclimated in the *** and pyridine were supple-mented to the synthetic wastewater at different *** optimum ratio of chemical oxygen demand(COD)to nitrate(C/N)was 8.4-8.9 for both denitrification and indole and pyridine *** a temperature of 28°C and pH of 7.0-7.5,the nitrate reductase activity(NRA)was in the best *** addition of pyridine could promote NRA and the degradation of *** the initial concentration of indole was 150 mg/L,the concentration ratio of indole to pyridine was in the range of *** optimum C/N conditions,the degradation of indole could be described with pseudo-zero-order *** was no accumulation of nitrite during the *** the concentration ratio of pyridine to indole was less than 0.25 with an increase in the pyridine proportion,there were more significant augment rates for NRA and the degradation of indole than the situation when the concentration ratio was more than 0.25.

关键词： indole pyridine promotive effect denitrification nitrate reductase activity

Responses　of　Two　Cultivars　of　Winter　Wheat　Differing　in　Sensitivity　to　Aluminum　Toxicity

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Pedosphere 1996年第3期 233-241页

作者： SHEN ZHEN-GUO LIAN CHUN-LAN and WU LIE-HONG(Nanjing Agricutural University, Nanjing 210014 China)

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关键词： aluminum toxicity free proline growth analysis nitrate reductase activity wheat

Influences of 5-aminolevulinic acid on the growth and physiological properties of winter oilseed rape（Brassica napus）responses to cold

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Influences of 5-aminolevulinic acid on the growth and physio...