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安徽医科大学学报 1985年第3期 1-4页

作者：王增贤杨兆莘安医寄生虫学教研室

结膜吸吮线虫成虫和初产蚴的体表超微结构用扫描电镜进行了观察,证实成虫口孔呈六边形。外环乳突4对,每对为大小各1个。头感器1对,呈裂隙状开口。颈乳头1对形似乳房状。成虫末端有1对呈新月形开口的尾感器。雌虫阴孔为纵形开口。雄虫短... 详细信息

结膜吸吮线虫成虫和初产蚴的体表超微结构用扫描电镜进行了观察,证实成虫口孔呈六边形。外环乳突4对,每对为大小各1个。头感器1对,呈裂隙状开口。颈乳头1对形似乳房状。成虫末端有1对呈新月形开口的尾感器。雌虫阴孔为纵形开口。雄虫短交合刺呈勺状,长交合刺经短交合刺的勺面伸出。初产蚴头部有3对齿状突起。体表环纹形态与成虫相似但较纤细。

关键词： Thelazia callipaeda Adult newborn larvae Scanning electron microscopy

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Proteomic analysis of differentially expressed proteins in the three developmental stages of Trichinella spiralis

Proteomic analysis of differentially expressed proteins in t...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Jingyi Liu Nianzhang Zhang Wenhui Li Li Li Hongbin Yan Zigang Qu Tingting Li Jianmin Cui Yangyang Wanzhong Jia Baoquan Fu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease

Trichinella spiralis, an intracellular parasitic nematode, can cause severe foodborne zoonosis, trichinellosis. The life cycle of T. spiralis consists of adult(Ad), muscle larvae(ML) and newborn larvae(NBL). The protein profiles in different developmental stages of the parasite remain unknown. In the present study, proteins from lysates of Ad, ML and NBL were identified by isobaric tags for relative and absolute quantitation(iTRAQ). A total of 4,691 proteins were identified in all the developmental stages, of which 1,067 proteins were differentially expressed(DEPs). DEPs in the three developmental stages of T. spiralis were involved in diverse functions in the life cycle, including regulation of worm growth and sexual maturity(e.g. major sperm protein), metabolism and utilization of carbohydrates, lipids and nucleotides(e.g. glucose-6-phosphate isomerase), modulation of host cell functions for the parasite benefit via excretory-secretory(E-S) antigens(e.g. serine protease), and other crucial developmental processes that occur at distinct stages. The number of up-regulated proteins in NBL was higher than that of the other two groups. The protein profiles from Ad, ML and NBL were compared in pairs. The identified proteins were involved in various functions of T. spiralis life cycle, including sexual maturity, metabolism, utilization of carbohydrates, lipids and nucleotides, and other crucial developmental processes that occur at distinct stages. The possible functions of the identified proteins were predicted by the gene function classification system GO, which has three ontologies including molecular function(16,015 proteins), cellular component(8,740 proteins) and biological process(4,676 proteins). Eight genes from DEPs designated E5S8I3 and E5SED3(major sperm protein, gene locus tag, Tsp0052 and Tsp2102), B7SIW4(serine protease gene locus tag, TspP-1.2), Q27071(53kD a E-S antigen, gene locus tag, Tspp53), E5SF13(zinc finger protein, gene locus tag Tsp2330), E5RY73

关键词： Trichinella spiralis iTRAQ adult muscle larvae newborn larvae proteomics

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