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Analysis of sensitivity and specificity:precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

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Forensic Sciences Research 2022年第2期7卷 228-237页

作者： Jiajia Niu Guoshuai An Zhen Gu Peng Li Qiqing Liu Rufeng Bai Junhong Sun Qiuxiang Du School of Forensic Medicine Shanxi Medical UniversityJinzhongChina Criminal Investigation Brigade Zhuji Public Security BureauZhujiChina 2011 Cooperative Innovation Center of Judicial Civilization BeijingChina Key Laboratory of Evidence Science China University of Political Science and LawMinistry of EducationBeijingChina

In this report,we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle,and to provide information for follow-up studies on neutrophils to estimate wound *** injury model was established,and skeletal muscle samples were obtained from the control group and contusion groups at 1,1.5,2,3,4,and 6 h,as well as at 1,3,5,and 15 d post-injury(n紏5 per group).The expression of nuclei and neutrophils was detected by hematoxylin and eosin(HE)staining and immunohistochemical(IHC)staining.A total of 20 injury site areas of 0.25mm^(2)(0.5mm0.5mm)were then randomly selected at all time points.A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative *** of five professional medical workers were considered the gold standard to measure the false positive rate(FPR),false negative rate(FNR),sensitivity,specificity,and area under the curve(AUC)of receiver operating characteristic(ROC)*** a result,with a staining area of neutrophils from 8 μm^(2) to 15 μm^(2),the FPR was 4.28%–12.14%,the FNR was 12.42%–64.08%,the sensitivity was 35.92%–87.58%,the specificity was 87.86%–95.72%,the Youden index was 0.316–0.754,the accuracy was 82.80%–88.30%,and the AUC was 0.771–*** AUC was largest when the cut-off value of the staining area was 12 mm^(2).Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil *** on the sensitivity and specificity,neutrophils can be accurately identified during regeneration of contused skeletal *** TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further ***,it will provide evidence for forensic pathology cases.

关键词： Forensic sciences forensic pathology immunohistochemistry TissueFAXS 200 digital pathological analysis system parameter optimization cut-off value of staining area neutrophil staining area

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