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Proteomic analysis of trans-hemispheric motor cortex reorganization following contralateral C7 nerve transfer

Neural Regeneration Research 2018年第2期13卷 331-339页

作者： Yin Yuan Xiu-yue Xu Jie Lao Xin Zhao Department of Hand Surgery Huashan HospitalFudan UniversityShanghaiChina Key Laboratory of Hand Reconstruction Ministry of HealthShanghaiChina Shanghai Key Laboratory of Peripheral Nerve&Microsurgery ShanghaiChina

Nerve transfer is the most common treatment for total brachial plexus avulsion injury. After nerve transfer, the movement of the injured limb may be activated by certain movements of the healthy limb at the early stage of recovery, i.e., trans-hemispheric reorganization. Pre- vious studies have focused on functional magnetic resonance imaging and changes in brain-derived neurotrophic factor and growth asso- ciated protein 43, but there have been no proteomics studies. In this study, we designed a rat model of total brachial plexus avulsion injury involving contralateral C7 nerve transfer. Isobaric tags for relative and absolute quantitation and western blot assay were then used to screen differentially expressed proteins in bilateral motor cortices. We found that most differentially expressed proteins in both cortices of upper limb were associated with nervous system development and function （including neuron differentiation and development, axonogenesis, and guidance）, microtubule and cytoskeleton organization, synapse plasticity, and transmission of nerve impulses. Two key differentially expressed proteins, neurofilament light （NFL） and Thy-1, were identified. In contralateral cortex, the NFL level was upregulated 2 weeks after transfer and downregulated at 1 and 5 months. The Thy-1 level was upregulated from 1 to 5 months. In the affected cortex, the NFL level increased gradually from 1 to 5 months. Western blot results of key differentially expressed proteins were consistent with the proteom- ic findings. These results indicate that NFL and Thy-1 play an important role in trans-hemispheric organization following total brachial plexus root avulsion and contralateral C7 nerve transfer.

关键词： nerve regeneration brachial plexus brain plasticity contralateral C,q cortex reorganization isobaric tags for relative and absolute quantitation proteomics nerve transfer neurofilament light Thy-1 neural regeneration

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Differential effect of ethanol intoxication on peripheral markers of cerebral injury in murine blunt traumatic brain injury

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Burns & Trauma 2021年第1期9卷 207-219页

作者： Zhenghui Li Jin Zhang Steffen Halbgebauer Akila Chandrasekar Rida Rehman Albert Ludolph Tobias Boeckers Markus Huber-Lang Markus Otto Francesco Roselli Florian olde Heuvel Department of Neurology Ulm UniversityZBMF-Helmholtzstrasse 8/189081 UlmGermany Department of Neurosurgery Kaifeng Central HospitalKaifeng475000 Henan China German Center for Neurodegenerative Diseases(DZNE) UlmGermany Institute of Anatomy and Cell Biology Ulm UniversityUlmGermanyM24Albert-Einstein Allee 1189081 UlmGermany Institute of Clinical and Experimental Trauma-Immunology University HospitalZBMF-Helmhotzstrasse 8/189081 UlmGermany Zoological Institute Braunschweig Cellular Neurobiology–BiozentrumSpielmannstrasse 738106 Braunschweig

Background:Blood-based biomarkers have proven to be a reliable measure of the severity and outcome of traumatic brain injury(TBI)in both murine models and *** particular,neuronspecific enolase(NSE),neurofilament light(NFL)and S100 beta(S100B)have been investigated in the clinical setting *** intoxication(EI)remains a significant comorbidity in TBI,with 30–40%of patients having a positive blood alcohol concentration *** effect of ethanol on blood-based biomarkers for the prognosis and diagnosis of TBI remains *** this study,we investigated the effect of EI on NSE,NFL and S100B and their correlation with blood–brain barrier integrity in a murine model of ***:We used ultra-sensitive single-molecule array technology and enzyme-linked immunosorbent assay methods to measure NFL,NSE,S100B and claudin-5 concentrations in plasma 3 hours ***:We showed that NFL,NSE and S100B were increased at 3 hours ***,ethanol blood concentrations showed an inverse correlation with NSE but not with NFL or ***-5 levels were increased post-injury but no difference was detected compared to ethanol *** increase in claudin-5 post-TBI was correlated with NFL but not with NSE or ***:Ethanol induces an effect on biomarker release in the bloodstream that is different from TBI not influenced by *** could be the basis of investigations into humans.

关键词： Traumatic brain injury Ethanol Biomarkers neurofilament light neuron-specific enolase S100B Claudin-5

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