检索结果-南通市图书馆

Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

引用

World Journal of Stem cells 2021年第5期13卷 452-469页

作者： Vladimir P Baklaushev Oleg V Durov Vladimir A Kalsin Eugene V Gulaev Sergey V Kim Ilya L Gubskiy Veronika A Revkova Ekaterina M Samoilova Pavel A Melnikov Dzhina D Karal-Ogly Sergey V Orlov Alexander V Troitskiy Vladimir P Chekhonin Alexander V Averyanov Jan-Eric Ahlfors Biomedical Research Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Neurosurgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBAMoscow 115682MoskvaRussia Department of Anesthesiology N.N.Blokhin Russian Cancer Research CentreMoscow 115478MoskvaRussia Radiology and Clinical Physiology Scientific Research Center Federal center of brain research and neurotechnologies of the Federal Medical Biological AgencyMoscow 117997Russia Department of Neurobiology Serbsky National Medical Research Center for Psychiatry and NarcologyMoscow 119992MoskvaRussia Department of Primatology Russian Acad Med SciResearch Institute of Medical PrimatologySochi 119992SochiRussia Department of Vascular Surgery Federal Research and Clinical Center of Specialized Medical Care and Medical TechnologiesFMBA of RussiaMoscow 115682MoskvaRussia Department of Basic and Applied Neurobiology V.P.Serbsky Federal Medical Research Center for Psychiatry and NarcologyRussia Department of Medical Nanobiotechnology Pirogov Russian National Research Medical University(RNRMU)Moscow 115682MoskvaRussia New World Laboratories Laval H7V 4A7QuebecCanada

BACKGROUND The development of regenerative therapy for human spinal cord injury(SCI)is dramatically restricted by two main challenges:the need for a safe source of functionally active and reproducible neural stem cells and the need of adequate animal models for preclinical *** reprogramming of somatic cells into neuronal and glial precursors might be a promising solution to the first *** use of non-human primates for preclinical studies exploring new treatment paradigms in SCI results in data with more translational relevance to human *** To investigate the safety and efficacy of intraspinal transplantation of directly reprogrammed neural precursor cells(drNPCs).METHODS Seven non-human primates with verified complete thoracic SCI were divided into two groups:drNPC group(n=4)was subjected to intraspinal transplantation of 5 million drNPCs rostral and caudal to the lesion site 2 wk post injury,and lesion control(n=3)was injected identically with the equivalent volume of *** Follow-up for 12 wk revealed that animals in the drNPC group demonstrated a significant recovery of the paralyzed hindlimb as well as recovery of somatosensory evoked potential and motor evoked potential of injured *** resonance diffusion tensor imaging data confirmed the intraspinal transplantation of drNPCs did not adversely affect the morphology of the central nervous system or cerebrospinal fluid *** immunohistochemical analysis showed that drNPCs maintained SOX2 expression characteristic of multipotency in the transplanted spinal cord for at least 12 wk,migrating to areas of axon growth *** Our data demonstrated that drNPC transplantation was safe and contributed to improvement of spinal cord function after acute SCI,based on neurological status assessment and neurophysiological recovery within 12 wk after *** functional improvement described was not associated with neuronal differentiation of th

关键词： Direct cell reprogramming neural precursor cells Directly reprogrammed neural precursor cells Spinal cord injury Nonhuman primates Regenerative therapy,Evoked potentials

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Functional electrical stimulation-facilitated proliferation and regeneration of neural precursor cells in the brains of rats with cerebral infarction

引用

neural Regeneration Research 2014年第3期9卷 243-251页

作者： Yun Xiang Huihua Liu Tiebin Yan Zhiqiang Zhuang Dongmei Jin Yuan Peng Department of Rehabilitation Medicine Sun Yat-sen Memorial Hospital Sun Yat-sen University Department of Rehabilitation Medicine Shenzhen Sixth People’s Hospital Department of Rehabilitation Medicine Guangzhou First People’s Hospital

Previous studies have shown that proliferation of endogenous neural precursor cells cannot alone compensate for the damage to neurons and axons. From the perspective of neural plastici- ty, we observed the effects of functional electrical stimulation treatment on endogenous neural precursor cell proliferation and expression of basic fibroblast growth factor and epidermal growth factor in the rat brain on the infarct side. Functional electrical stimulation was performed in rat models of acute middle cerebral artery occlusion. Simultaneously, we set up a placebo stimulation group and a sham-operated group. Immunohistochemical staining showed that, at 7 and 14 days, compared with the placebo group, the numbers of nestin （a neural precursor cell marker）-positive cells in the subgranular zone and subventricular zone were increased in the functional electrical stimulation treatment group. Western blot assays and reverse-transcription PCR showed that total protein levels and gene expression of epidermal growth factor and basic fibroblast growth factor were also upregulated on the infarct side. Prehensile traction test results showed that, at 14 days, prehension function of rats in the functional electrical stimulation group was significantly better than in the placebo group. These results suggest that functional electrical stimulation can promote endogenous neural precursor cell proliferation in the brains of acute cerebral infarction rats, enhance expression of basic fibroblast growth factor and epidermal growth factor, and improve the motor function of rats.

关键词： nerve regeneration brain injury functional electrical stimulation neural precursor cells neurogenesis basic fibroblast growth factor epidermal growth factor nestin stroke rats NSFC grant neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Do neural precursor cells exist in a distal neurogenic region following cerebral hemorrhage?

引用

neural Regeneration Research 2010年第5期5卷 325-330页

作者： Chaoxian Yang Ling Zhou Li Deng Xiaoqing Gao Department of Neurobiology Luzhou Medical College Luzhou 646000 Sichuan Province China Department of Endocrinology Affiliated Hospital of Luzhou Medical College Luzhou 646000 Sichuan Province China

BACKGROUND： Cerebral injury in adult mammals can induce neural precursor cells （NPCs） to proliferate and migrate towards the focal zone, but it is unclear whether endogenous NPCs can migrate towards regions distal to the hemorrhagic focus or whether NPCs differentiate in the peripheral hemorrhagic region. OBJECTIVE： To investigate the distribution of endogenous NPCs in different brain regions of rats with experimental cerebral hemorrhage, as well as NPC proliferation and differentiation with time. DESIGN, TIME AND SE＇B＇ING： A randomized, controlled animal experiment was performed at the Department of Neurobiology, Luzhou Medical College, between January 2007 and October 2008. MATERIALS： Bromodeoxyuridine （BrdU） was purchased from Roche, Germany. Mouse anti-rat BrdU monoclonal antibody, rabbit anti-nestin polyclonal antibody, rabbit anti-neuron specific enolase （NSE） polyclonal antibody were purchased from Wuhan Boster, China. Rabbit anti-glial fibrillary acidic protein （GFAP） polyclonal antibody was purchased from Sigma, USA. METHODS： Thirty-five adult Sprague Dawley rats were randomly divided into three groups：（1） cerebral hemorrhage group （n = 25）, rats were stereotaxically administered 50 p L autologous arterial blood via the dorsal caudate putamen to induce cerebral hemorrhage; （2） sham-surgery group （n = 5）, rats underwent surgery but did not receive blood injection; （3） blank control group （n = 5）, rats received no surgery and blood administration. At 2 hours after surgery, all rats were intraperitoneally administered BrdU. MAIN OUTCOME MEASURES： Distribution and proliferation of BrdU-positive cells were observed by immunohistochemical staining. BrdU-positive cell differentiation into neurons and glial cells in the peripheral hemorrhagic region was detected by double-label immunofluorescence. RESULTS： Immunohistochemistry results revealed that BrdU-positive cells existed not only in the peripheral hemorrhagic region, such as the subependymal layer and hippoca

关键词： rats cerebral hemorrhage neural precursor cells differentiation distal neuroyenic region distal neurogenic region

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Expression and regulation of versican in neural precursor cells and their lineages

引用

Acta Pharmacologica Sinica 2007年第10期28卷 1519-1530页

作者： Wen-li GU~(2,3),Sai-li FU~2,Yan-xia WANG~2,Ying LI~2,Xiao-fei WANG~2,Xiao-ming XU~(2,4),Pei-hua LU~(2,5)~2 Department of Neurobiology,Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China ~3 Department of ClinicalLaboratory,Ninth People's Hospital of Shanghai,Shanghai Jiao Tong University,Shanghai 200011,China ~4 Department of Neurological Surgery,University of Louisville School of Medicine,Louisville,Kentucky 40292,USA ment of Neurobiology Shanghai Jiao Tong University School of Medicine Shanghai 200025 China. peihualua3@***.

Aim:To have a better understanding of the expression and regulation of versicanisoforms in neural precursor cells (NPC) and ***:By immunocytochemistry,RT-PCR,and real-time PCR,we examined the tem-poral expression of versican in NPC isolated from embryonic d 16 rats as wellas in oligodendrocyte (OL) lineage cells induced to differentiate from NPC,which mimicked the oligodendrogliogenesis in ***:We found thatversican was constitutively expressed in NPC and their lineage cells,includingneurons,astrocytes,and *** addition,2 versican isoforms,V1/V0 and V2,were found to express at low levels in NPC,but at significantly higher levels inOL lineage *** peak expression of versican V2 was found at the oligo-dendrocyte precursor cell ***,the treatment of 2 pro-inflam-matory cytokines,TNF-α and IFN-γ,enhanced the transcription of versican V2in NPC in a dose-dependent manner,but showed no effect on V1/V0 ***:Taken together,our results demonstrate that versican,particularlythe inhibitory V2 isoform,is increasingly expressed in OL lineage cells inducedto differentiate from *** increase in versican V2 expression after cytokinestimulation implies the interplay between the injury-induced upregulation ofinflammatory cytokines and chondroitin sulfate proteoglycan-mediated inhibi-tion of axonal regeneration after central nervous system injury.

关键词： chondroitin sulfate proteoglycans neural precursor cells oligodendrocyte precursor cells oligodendrocytes pro-inflammatory cytokines versican

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

The involvement of SVZ-derived neural precursor cells in the epileptogenicity of focal cortical dysplasia

The involvement of SVZ-derived neural precursor cells in the...

引用

中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Hai-Feng SHU~1,Chun-Qing ZHANG~1,Qin YIN~2,Ning AN~1,Shi-Yong LIU~1,Zhuan ZHOU~3,Hui YANG~(1,*) 1 Department of Neurosurgery,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China 2 Department of Rehabilitation,Southwest Hospital,Third Military Medical University,Chongqing 400038,China 3 Institute of Molecular Medicine and State Key Laboratory of Membrane Bioengineering,Peking University,Beijing, 100871,China

＜正＞Objective Focal cortical dysplasia（FCD） is a common cause of medically intractable epilepsy in pediatric *** has indicated that the abnormal cells within the cortical lesions of FCD,which are closely correlated with the severity of seizure,are derived in part from the neural precursor cells（NPCs） of subventricular zone（SVZ）. Therefore,to observe the migration,differentiation and the electrophysiological features of the SVZ-derived cells within the cortical dysplastic lesions of FCD will help us better understand the epileptogenicity of *** We created an FCD rat model by focal contact of a frozen metal probe on the scalp immediately after birth,in which the SVZ-derived NPCs were labeled with DiI through intracerebroventricular *** neurobiological features of DiI+ cells within the freezing-induced cortical lesions were studied by morphological and electrophysiological *** The DiI+ cells,which form a migratory stream from SVZ to cortical lesions,could be continuously observed from P10 to *** immunofluorescence results showed that the majority of SVZ-derived NPCs in the cortical lesions differentiated into glial cells and neurons,and to format synaptic connections with the surround cells, suggesting the possibility of these cells to influence the local neural *** studies indicated that the SVZ-NPCs-derived neurons showed high excitability and received more excitatory input,while the feedback suppression to the presynaptic cells *** These data suggested that the high excitability of SVZNPCs -derived neurons would result in the hyperexcitability of the local neural circuit,and consequently trigger the kindling of the epileptiform discharge.

关键词： focal cortical dysplasia neural precursor cells epilepsy

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Regulator of G protein signaling 6 mediates exercise-induced recovery of hippocampal neurogenesis,learning,and memory in a mouse model of Alzheimer’s disease

引用

neural Regeneration Research 2025年第10期20卷 2969-2981页

作者： Mackenzie M.Spicer Jianqi Yang Daniel Fu Alison N.DeVore Marisol Lauffer Nilufer S.Atasoy Deniz Atasoy Rory A.Fisher Department of Neuroscience and Pharmacology University of Iowa Carver College of MedicineIowa CityIAUSA Iowa Neuroscience Institute University of Iowa Carver College of MedicineIowa CityIAUSA Interdisciplinary Graduate Program in Molecular Medicine University of Iowa Carver College of MedicineIowa CityIAUSA neural Circuits and Behavior Core University of Iowa Carver College of MedicineIowa CityIAUSA

Hippocampal neuronal loss causes cognitive dysfunction in Alzheimer’s *** hippocampal neurogenesis is reduced in patients with Alzheimer’s *** stimulates adult hippocampal neurogenesis in rodents and improves memory and slows cognitive decline in patients with Alzheimer’s ***,the molecular pathways for exercise-induced adult hippocampal neurogenesis and improved cognition in Alzheimer’s disease are poorly ***,regulator of G protein signaling 6(RGS6)was identified as the mediator of voluntary running-induced adult hippocampal neurogenesis in ***,we generated novel RGS6fl/fl;APP_(SWE) mice and used retroviral approaches to examine the impact of RGS6 deletion from dentate gyrus neuronal progenitor cells on voluntary running-induced adult hippocampal neurogenesis and cognition in an amyloid-based Alzheimer’s disease mouse *** found that voluntary running in APP_(SWE) mice restored their hippocampal cognitive impairments to that of control *** cognitive rescue was abolished by RGS6 deletion in dentate gyrus neuronal progenitor cells,which also abolished running-mediated increases in adult hippocampal *** hippocampal neurogenesis was reduced in sedentary APP_(SWE) mice versus control mice,with basal adult hippocampal neurogenesis reduced by RGS6 deletion in dentate gyrus neural precursor ***6 was expressed in neurons within the dentate gyrus of patients with Alzheimer’s disease with significant loss of these RGS6-expressing ***,RGS6 mediated voluntary running-induced rescue of impaired cognition and adult hippocampal neurogenesis in APP_(SWE) mice,identifying RGS6 in dentate gyrus neural precursor cells as a possible therapeutic target in Alzheimer’s disease.

关键词： adult hippocampal neurogenesis Alzheimer’s disease dentate gyrus exercise learning/memory neural precursor cells regulator of G protein signaling 6(RGS6)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Spreading depression and focal venous cerebral ischemia enhance cortical neurogenesis

引用

neural Regeneration Research 2017年第8期12卷 1278-1286页

作者： Ryo Tamaki Samuel Ige Orie Beat Alessandri Oliver Kempski Axel Heimann Department of Neurosurgery Nara Medical University University Medical Center of the Johannes Gutenberg-University of Mainz Institute for Neurosurgical Pathophysiology

Endogenous neurogenesis can arise from a variety of physiological stimuli including exercise, learning, or ＂enriched environment＂ as well as pathological conditions such as ischemia, epilepsy or cortical spreading depression. Whether all these conditions use a common trigger to set off endogenous neurogenesis is yet unclear. We hypothesized that cortical spreading depression（CSD） induces neurogenesis in the cerebral cortex and dentate gyrus after cerebral venous ischemia. Forty-two Wistar rats alternatively underwent sham operation（Sham）, induction of ten CSDs or venous ischemia provoked via occlusion of two adjacent superficial cortical vein followed by ten induced CSDs（CSD ＋ 2-VO）. As an additional control, 15 na？ve rats received no intervention except 5-bromo-2′-deoxyuridine（Brd U） treatment for 7 days. Sagittal brain slices（40 μm thick） were co-stained for Brd U and doublecortin（DCX; new immature neuronal cells） on day 9 or Neu N（new mature neuronal cells） on day 28. On day 9 after sham operation, cell proliferation and neurogenesis occurred in the cortex in rats. The sole induction of CSD had no effect. But on days 9 and 28, more proliferating cells and newly formed neurons in the ipsilateral cortex were observed in rats subjected to CSD ＋ 2VO than in rats subjected to sham operation. On days 9 and 28, cell proliferation and neurogenesis in the ipsilateral dentate gyrus was increased in sham-operated rats than in na？ve rats. Our data supports the hypothesis that induced cortical neurogenesis after CSD ＋ 2-VO is a direct effect of ischemia, rather than of CSD alone.

关键词： nerve regeneration cortical spreading depression two-vein occlusion adult neurogenesis stem cells cerebral cortex neural precursor cells neuron penumbra neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Adult neurogenesis in the primate hippocampus

引用

Zoological Research 2023年第2期44卷 315-322页

作者： Ye Li Na-Na Xu Zhao-Zhe Hao Sheng Liu State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangdong Provincial Key Laboratory of Ophthalmology and Visual ScienceGuangzhouGuangdong 510060China Guangdong Province Key Laboratory of Brain Function and Disease GuangzhouGuangdong 510080China

Adult hippocampal neurogenesis(AHN) is crucial for learning,memory,and *** of AHN may lead to reduced cognitive abilities and neurodegenerative disorders,such as Alzheimer’s *** studies on rodent AHN have clarified the developmental and maturation processes of adult neural stem/progenitor ***,to what extent these findings apply to primates remains *** advances in next-generation sequencing technologies have enabled in-depth investigation of the transcriptome of AHN-related populations at single-cell ***,we summarize studies of AHN in *** suggest that neurogenesis is largely shared across species,but substantial differences also *** gene expression patterns in primates differ from those of *** with rodents,the primate hippocampus has a higher proportion of immature dentate granule cells and a longer maturation period of newly generated granule *** research on species divergence may deepen our understanding of the mechanisms underlying adult neurogenesis in primates.

关键词： neural precursor cells Immature granule cells Sc-RNA-seq

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Behavioral outcomes of combination therapy after chronic spinal cord injury

Behavioral outcomes of combination therapy after chronic spi...

引用

2012第二届国际神经再生高峰论坛

作者： Y.Jin J.Bouyer J.S.Shumsky I.Fischer Department of Neurobiology and Anatomy Drexel University College of Medicine

Few studies addressed chronic spinal cord injury due to the experimental complexities and the daunting challenges associated with axonal growth through the scar formed during chronic injury. Our previous studies demonstrated that grafting neural precursor cells （NPCs） into a thoracic contused spinal cord 9 days after injury can improve bladder, sensory and motor functions. We reasoned that for chronic injury we need to reduce the scar and increase axonal growth and therefore transplanted NPC combined with a lentivirus （LV） vector that expresses chondroitinase （Chase）, to digest the gliotic scar, and neurotrophic factors （including BDNF and NT-3）, to stimulate neuronal plasticity. The main aim of the study was to determine whether such a combination strategy will improve functional recovery after chronic spinal cord injury. Adult female Sprague-Dawley rats received a moderate contusion lesion （NYU, 10g, 25 mm） at T 10 . Rats were divided into four groups matched by BBB score 2-3 days post-injury and recovered 12 weeks before receiving the following treatments: medium, NPC, NPC/Chase （NPC + LV-Chase）, and NPC/Chase/NTF （NPC + LV-Chase + LV-BDNF/NT-3）. Animals survived for 8 weeks after transplantation. NPCs （1 × 106 /10 μL/rat） were prepared from embryonic day 13.5 alkaline phosphatase transgenic rats and cultured no more than 5 days before transplantation. One week later, the lentivirus vectors were injected into center, rostral, and caudal relative to the transplant. Behavioral tests, including BBB, grid （motor function）, heat and Von Frey （sensory function）, were performed before and after injury, and following transplantation once a week until animals were sacrificed. Horizontal sections including lesion/transplant were stained with Nissl/myelin for tissue volume measurements. We found that combination treatment （NPC/Chase/NTF） reduced the cyst volume;however, there were no differences in lesion/transplant size or spared gray and white matter amongst the groups. A

关键词： chronic spinal cord injury behavioral outcomes neural precursor cells chondroitinase neurotrophic factors

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Effects of Agmatine on the Proliferation of progenitor cells from Neonatal Rat Hippocampus

Effects of Agmatine on the Proliferation of progenitor Cells...

引用

第十五届国际药理学大会

作者： LIU Ying. LI Yun-Feng. LI Jin (Beijing Institute of Pharmacology and Toxicology. Beijing 100850. China)

＜正＞ AIM: To study the effects of agmatine on the proliferation ability of neural progenitor cells from neonatal rat hippocampus. METHODS: Hippocampus of neonatal rat was isolated and made into single - cell suspension,which was cultured in serum- free medium. The survival rate of neural precursor cells incubated with various concentrations of agmatine and efaroxan was tested by CCK - 8 kit assay and 3H - thymidine incorporation assay. RESULTS: cells could continuously proliferate and cultured as floating neurospheres. Agmatine at 1μmol·L-1 and 10μmol·L-1 enhanced the survival rate of neural precursor cells by CCK- 8 kit assay,and imidazoline- 1 receptor antagonist efaroxan blocked the proliferation effect of agmatine. The same results were observed by 3H - thymidine incorporation assay. CONCLUSION: Agmatine were found to increase the proliferation of neural precursor cells and efaroxan can block the proliferation effect. It suggested that imidazoline - 1 receptor may be related to the proliferation effect.

关键词： neural precursor cells proliferation ability agmatine imidazoline-1 receptor

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：