检索结果-南通市图书馆

Ouabain-Induced Auditory Nerve degeneration in Congenic Ly5.1 Mice

在线全文

学校读者我要写书评

暂无评论

Journal of Otology 2011年第2期6卷 19-28页

作者： Lauren A.Kilpatrick Devadoss J Samuvel ZHU Ju-hong Nancy Smythe LANG Hai-nan Department of Pathology and Laboratory Medicine Medical University of South Carolina Charleston SC 29425 United States Department of Otolaryngology - Heary & Neck Surgery Medical University of South Carolina Charleston SC 29425 United States

The Ly5.1 mouse,also termed ***-Ptprca Pepcb/BoyJ,is a congenic strain widely used as a recipient in animal studies of bone marrow *** previous study documented that a majority of type I spiral ganglion neurons (SGNs) in the apical turns of Ly5.1 mice are unmyelinated and aggregate into neuronal clusters,similar to the spiral ganglion in the human ***,a well known Na-K ATPase inhibitor,has been shown to induce neuronal degeneration in a variety of neural tissues including the adult gerbil and CBA/CaJ mouse spiral ***,functional and pathological changes of the auditory nerves in young-adult Ly5.1 mice were examined at 3,7 and 14 days after ouabain *** to observations in CBA/CaJ mice,ouabain application selectively removed type I SGNs,resulting in an immense decline of the auditory nerve *** of glial cells was seen in the injured auditory nerves at 7 days after ouabain *** data indicate that the 'human-like' features of unmyelinated type I SGNs have no protective impact on the fate of SGNs after ouabain *** incorporating bromodeoxyuridine (BrdU) and expressing Sox2 were also counted in the auditory nerves of control and ouabain-treated *** number of Sox2+ glial cells significantly increased at 3 and 7 days ***,the highest density of BrdU+ cells appeared in the apical turn of the injured auditory nerve shortly after ouabain exposure,suggesting that the pattern of SGN loss at the apical turn in Ly5.1 mouse may have some impact on the reaction of non-neuronal cells in response to acute ototoxic drug exposure in the auditory nerve.

关键词： primary auditory nerve Ly5.1 mouse ouabain neural degeneration Sox2 BrdU glial cells

Zhichan decoction induces differentiation of dopaminergic neurons in Parkinson's disease rats after neural stem cell transplantation

在线全文

学校读者我要写书评

暂无评论

neural Regeneration Research 2014年第9期9卷 931-936页

作者： Huifen Shi Jie Song Xuming Yang Department of Neurology Shanghai Municipal Hospital of Traditional Chinese Medicine Department of Encephalopathy Liu’an Hospital of Traditional Chinese Medicine College of Acupuncture and Massage Shanghai University of Traditional Chinese Medicine

The goal of this study was to increase the dopamine content and reduce dopaminergic metabolites in the brain of Parkinson’s disease rats. Using high-performance liquid chromatography, we found that dopamine and dopaminergic metabolite（dihydroxyphenylacetic acid and homovanillic acid） content in the midbrain of Parkinson’s disease rats was increased after neural stem cell transplantation ＋ Zhichan decoction, compared with neural stem cell transplantation alone. Our genetic algorithm results show that dihydroxyphenylacetic acid and homovanillic acid levels achieve global optimization. neural stem cell transplantation ＋ Zhichan decoction increased dihydroxyphenylacetic acid levels up to 10-fold, while transplantation alone resulted in a 3-fold increment. Homovanillic acid levels showed no apparent change. Our experimental findings show that after neural stem cell transplantation in Parkinson’s disease rats, Zhichan decoction can promote differentiation of neural stem cells into dopaminergic neurons.

关键词： nerve regeneration traditional Chinese medicine neurodegeneration Parkinson’s disease rat model Zhichan decoction stem cell transplantation dopamine metabolite dihydroxyphenylacetic acid homovanillic acid curve fitting equation genetic algorithm optimization model NSFC grant neural degeneration

Changes in the blood-nerve barrier after sciatic nerve cold injury:indications supporting early treatment

在线全文

学校读者我要写书评

暂无评论

neural Regeneration Research 2015年第3期10卷 419-424页

作者： Hao Li Jian-ping Jia Min Xu Lei Zhang Department of Neurology the First People’s Hospital of Yibin Department of Neurology Xuanwu Hospital Capital Medical University Department of Pharmacy the First People’s Hospital of Yibin

Severe edema in the endoneurium can occur after non-freezing cold injury to the peripheral nerve, which suggests damage to the blood-nerve barrier. To determine the effects of cold injury on the blood-nerve barrier, the sciatic nerve on one side of Wistar rats was treated with low tem- peratures （3-5℃） for 2 hours. The contralateral sciatic nerve was used as a control. We assessed changes in the nerves using Evans blue as a fluid tracer and morphological methods. Excess fluid was found in the endoneurium 1 day after cold injury, though the tight junctions between cells remained closed. From 3 to 5 days after the cold injury, the fluid was still present, but the tight junctions were open. Less tracer leakage was found from 3 to 5 days after the cold injury compared with 1 day after injury. The cold injury resulted in a breakdown of the blood-nerve barrier func- tion, which caused endoneurial edema. However, during the early period, the breakdown of the blood-nerve barrier did not include the opening of tight junctions, but was due to other factors. Excessive fluid volume produced a large increase in the endoneurial fluid pressure, prevented liquid penetration into the endoneurium from the microvasculature. These results suggest that drug treatment to patients with cold injuries should be administered during the early period after injury because it may be more difficult for the drug to reach the injury site through the microcirculation after the tissue fluid pressure becomes elevated.

关键词： nerve regeneration peripheral nerve injury sciatic nerve hypothermia blood-nervebarrier Evans blue tracer neural degeneration

Mechanical properties of nerve roots and rami radiculares isolated from fresh pig spinal cords

在线全文

学校读者我要写书评

暂无评论

neural Regeneration Research 2015年第11期10卷 1869-1873页

作者： Norihiro Nishida Tsukasa Kanchiku Junji Ohgi Kazuhiko Ichihara Xian Chen Toshihiko Taguchi Department of Orthopedic Surgery Yamaguchi University Graduate School of Medicine Faculty of Engineering Yamaguchi University Non-Profit Organization Corporation Japan Orthopedic Biomechanics Institute Faculties of Engineering Yamaguchi University

No reports have described experiments designed to determine the strength characteristics of spinal nerve roots and rami radiculares for the purpose of explaining the complexity of symptoms of medullary cone lesions and cauda equina syndrome. In this study, to explain the pathogenesis of cauda equina syndrome, monoaxial tensile tests were performed to determine the strength characteristics of spinal nerve roots and rami radiculares, and analysis was conducted to evaluate the stress-strain relationship and strength characteristics. Using the same tensile test device, the nerve root and ramus radiculares isolated from the spinal cords of pigs were subjected to the tensile test and stress relaxation test at load strain rates of 0.1, 1, 10, and 100 s-1 under identical settings. The tensile strength of the nerve root was not rate dependent, while the ramus radiculares tensile strength tended to decrease as the strain rate increased. These findings provide important insights into cauda equina symptoms, radiculopathy, and clinical symptoms of the medullary cone.

关键词： nerve regeneration cauda equina syndrome monoaxial tensile tests radiculopathy strength characteristics stress-strain relationship lumbar spinal canal stenosis paralysis spinal cord neural degeneration