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Effects of neuregulin-1 on autonomic nervous system remodeling post-myocardial infarction in a rat model

Neural Regeneration Research 2017年第11期12卷 1905-1910页

作者： Xin Lai Liang Zhong Hai-xia Fu Song Dang Xin Wang Ning Zhang Gao-ke Feng Zi-qiang Liu Xi Wang Long Wang Department of Cardiology Renmin Hospital of Wuhan UniversityWuhanHubei ProvinceChina Cardiovascular Research Institute Wuhan UniversityWuhanHubei ProvinceChina Hubei Key Laboratory of Cardiology WuhanHubei ProvinceChina Wuhan Medical&Healthcare Center for Women and Children WuhanHubei ProvinceChina Department of Cardiology Henan Province People's HospitalZhengzhouHenan ProvinceChina

Sympathetic nerve and vagus nerve remodeling play an important part in cardiac function post-myocardial infarction （MI）. Increasing evidence indicates that neuregulin-1 （NRG-1） improves cardiac function following heart failure. Since its impact on cardiac function and neural remodeling post-MI is poorly understood, we aimed to investigate the role of NRG-1 in autonomic nervous system remodeling post-MI. Forty-five Sprague-Dawley rats were equally randomized into three groups： sham （with the left anterior descending coronary artery exposed but without ligation）, MI （left anterior descending coronary artery ligation）, and MI plus NRG-1 （left anterior descending coronary artery ligation followed by intraperitoneal injection of NRG-1 （10 lag/kg, once daily for 7 days））. At 4 weeks after MI, echocardi- ography was used to detect the rat cardiac function by measuring the left ventricular end-systolic inner diameter, left ventricular diastolic diameter, left ventricular end-systolic volume, left ventricular end-diastolic volume, left ventricular ejection fraction, and left ventricular fractional shortening, mRNA and protein expression levels of tyrosine hydroxylase, growth associated protein-43 （neuronal specific pro- tein）, nerve growth factor, choline acetyltransferase （vagus nerve marker）, and vesicular acetylcholine transporter （cardiac vagal nerve fiber marker） in ischemic myocardia were detected by real-time PCR and western blot assay to assess autonomous nervous remodeling. After MI, the rat cardiac function deteriorated significantly, and it was significantly improved after NRG-1 injection. Compared with the MI group, mRNA and protein levels of tyrosine hydroxylase and growth associated protein-43, as well as choline acetyltransferase mRNA level significantly decreased in the MI plus NRG-1 group, while mRNA and protein levels of nerve growth factor and vesicular acetylcholine transporters, as well as choline acetyltransferase protein level slightly decreased. Our results indicate that NRG- 1 can improve cardiac

关键词： nerve remodeling myocardial infarction neuregulin-1 sympathetic nerve vagus nerve animal model real-time PCR westernblot assay cardiac function echocardiography

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Short-term observations of the regenerative potential of injured proximal sensory nerves crossed with distal motor nerves

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Neural Regeneration Research 2017年第7期12卷 1172-1176页

作者： Xiu-xiu Zhang Yu-hui Kou Xiao-feng Yin Bao-guo Jiang Pei-xun Zhang Department of Orthopedics and Trauma Peking University People's Hospital Beijing China

Motor nerves and sensory nerves conduct signals in different directions and function in different ways.In the surgical treatment of peripheral nerve injuries,the best prognosis is obtained by keeping the motor and sensory nerves separated and repairing the nerves using the suture method.However,the clinical consequences of connections between sensory and motor nerves currently remain unknown.In this study,we analyzed the anatomical structure of the rat femoral nerve,and observed the motor and sensory branches of the femoral nerve in the quadriceps femoris.After ligation of the nerves,the proximal end of the sensory nerve was connected with the distal end of the motor nerve,followed by observation of the changes in the newly-formed regenerated nerve fibers.Acetylcholinesterase staining was used to distinguish between the myelinated and unmyelinated motor and sensory nerves.Denervated muscle and newly formed nerves were compared in terms of morphology,electrophysiology and histochemistry.At 8 weeks after connection,no motor nerve fibers were observed on either side of the nerve conduit and the number of nerve fibers increased at the proximal end.The proportion of newly-formed motor and sensory fibers was different on both sides of the conduit.The area occupied by autonomic nerves in the proximal regenerative nerve was limited,but no distinct myelin sheath was visible in the distal nerve.These results confirm that sensory and motor nerves cannot be effectively connected.Moreover,the change of target organ at the distal end affects the type of nerves at the proximal end.

关键词： nerve regeneration nerve remodeling peripheral nerve acetylcholinesterase staining muscle denervation neural anastomosis nerveconduit neural regeneration

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