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Electrodeposition of chitosan/graphene oxide conduit to enhance peripheral nerve regeneration

Neural Regeneration Research 2023年第1期18卷 207-212页

作者： Ya-Nan Zhao Ping Wu Zi-Yuan Zhao Fei-Xiang Chen Ao Xiao Zhi-Yi Yue Xin-Wei Han Yong Zheng Yun Chen Department of Biomedical Engineering and Hubei Province Key Laboratory of Allergy and Immune Related Disease School of Basic Medical SciencesWuhan UniversityWuhanHubei ProvinceChina Department of Interventional Radiology The First Affiliated Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Clinical Medical College Hubei University of Science and TechnologyXianningHubei ProvinceChina

Currently available commercial nerve guidance conduits have been applied in the repair of peripheral nerve defects.However,a conduit exhibiting good biocompatibility remains to be developed.In this work,a series of chitosan/graphene oxide(GO)films with concentrations of GO varying from 0-1 wt%(collectively referred to as CHGF-n)were prepared by an electrodeposition technique.The effects of CHGF-n on proliferation and adhesion abilities of Schwann cells were evaluated.The results showed that Schwann cells exhibited elongated spindle shapes and upregulated expression of nerve regeneration-related factors such as Krox20(a key myelination factor),Zeb2(essential for Schwann cell differentiation,myelination,and nerve repair),and transforming growth factorβ(a cytokine with regenerative functions).In addition,a nerve guidance conduit with a GO content of 0.25%(CHGFC-0.25)was implanted to repair a 10-mm sciatic nerve defect in rats.The results indicated improvements in sciatic functional index,electrophysiology,and sciatic nerve and gastrocnemius muscle histology compared with the CHGFC-0 group,and similar outcomes to the autograft group.In conclusion,we provide a candidate method for the repair of peripheral nerve defects using free-standing chitosan/GO nerve conduits produced by electrodeposition.

关键词： chitosan electrodeposition free-standing graphene oxide nerve conduit nerve factors Schwann cells tissue engineerin

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The engine initiating tissue regeneration:does a common mechanism exist during evolution?

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Cell Regeneration 2021年第1期10卷 127-138页

作者： Yanmei Liu Wilson Pak-KinLou Ji-Feng Fei Key Laboratory of Brain Cognition and Education SciencesMinistry of EducationInstitute for Brain Research and RehabilitationSouth China Normal University510631 GuangzhouChina School of Life Sciences South China Normal University510631 GuangzhouChina Research Institute of Molecular Pathology(IMP) Vienna Biocenter(VBC)ViennaAustria Guangdong Provincial People’s Hospital Guangdong Academy of Medical Sciences510080 GuangzhouChina

A successful tissue regeneration is a very complex process that requires a precise coordination of many molecular,cellular and physiological events.One of the critical steps is to convert the injury signals into regeneration signals to initiate tissue regeneration.Although many efforts have been made to investigate the mechanisms triggering tissue regeneration,the fundamental questions remain unresolved.One of the major obstacles is that the injury and the initiation of regeneration are two highly coupled processes and hard to separate from one another.In this article,we review the major events occurring at the early injury/regeneration stage in a range of species,and discuss the possible common mechanisms during initiation of tissue regeneration.

关键词： Tissue regeneration ROS Immune response nerve factors Cell types Regeneration initiating factors Injury

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Combination of bone marrow mesenchymal stem cells and brain-derived neurotrophic factor for treating spinal cord injury

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Neural Regeneration Research 2010年第7期5卷 491-495页

作者： Dengliang Wang Dezhi Kang Jianhua Lin Lianghong YU Zhangya Lin Zhaoyang Wu Department of Neurosurgery First Hospital of Fujian Medical University Fuzhou 350004 Fujian Province China Department of Orthopedics First Hospital of Fujian Medical University Fuzhou 350004 Fujian Province China

BACKGROUND： Because bone marrow mesenchymal stem cells （BMSCs） do not secrete sufficient brain-derived neurotrophic factor （BDNF）, the use of exogenous BDNF could improve microenvironments in injured regions for BMSCs differentiation. OBJECTIVE： To analyze recovery of the injured spinal cord following BMSCs venous transplantation in combination with consecutive injections of BDNF. DESIGN, TIME AND SETTING： A randomized, controlled animal experiment was performed at the Central Laboratory of First Hospital and Anatomical Laboratory, Fujian Medical University from October 2004 to May 2006. MATERIALS： Human BDNF was purchased from Sigma, USA. METHODS： A total of 44 New Zealand rabbits were randomly assigned to model （n = 8）, BDNF （n = 12）, BMSC （n= 12）, and BMSC＋BDNF （n= 12） groups. Spinal cord （I-2）injury was established with the dropping method. The model group rabbits were injected with 1 mL normal saline via the ear margin vein; the BDNF group was subdurally injected with 100 μg/d human BDNF for 1 week; the BMSC group was injected with 1 mL BMSCs suspension （2 × 10^6/mL） via the ear margin vein; and the BMSC＋BDNF group rabbits were subdurally injected with 100 μg/d BDNF for 1 week, in addition to BMSCs suspension via the ear margin vein. MAIN OUTCOME MEASURES： BMSCs surface markers were detected by flow cytometry. BMSCs differentiation in the injured spinal cord was detected by immunofluorescence histochemistry. Functional and structural recovery, as well as morphological changes, in the injured spinal cord were respectively detected by Tarlov score, horseradish peroxidase retrograde tracing, and hematoxylin ＆ eosin staining methods at 1, 3, and 5 weeks following transplantation. RESULTS： Transplanted BMSCs differentiated into neuronal-like cells in the injured spinal cord at 3 and 5 weeks following transplantation. Neurological function and pathological damage improved following BMSC ＋ BDNF treatment compared with BDNF or BMSC alone （P 〈 0.01 or P 〈 0.05）. CONCLUSION： BMSCs venous transplantation in combination

关键词： bone marrow mesenchymal stem cells brain-derived neurotrophic factor cell transplantation differentiation nerve factors spinal cord injury neural regeneration

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TrkB and p-trkB expression in brain-derived neurotrophic factor-pretreated rat retina following acute high intraocular pressure

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Neural Regeneration Research 2010年第12期5卷 911-916页

作者： Lizhu Jiang Jufang Huang Hui Wang Dan Chen Hongnian Zhao Department of Neuropsychopathy Clinical Medical School Daft University Dali 671000 Yunnan Province China Department of Anatomy & Neurobiology Xiangya Medical School Central South University Changsha 410013 Hunan Province China

BACKGROUND： Exogenous brain-derived neurotrophic factor （BDNF） promotes retinal ganglion cell survival. However, the protective mechanisms remain unclear. OBJECTIVE： To investigate changes in retinal tyrosine kinase receptor B （trkB） expression and effects of exogenous BDNF on trkB activation in a rat model of acute high intraocular pressure （HtOP）. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Department of Anatomy and Neurobiology, Xiangya Medical School, Central South University from January 2004 to August 2006. MATERIALS： Rabbit anti-BDNF and anti-trkB.FL（full-length） polyclonal antibodies were purchased from Santa Cruz Biotechnology, USA; rabbit anti-p-trkB polyclonal antibodies were purchased from Cellsignal, USA. METHODS： A total of 48 healthy, adult, Sprague Dawiey rats were randomly assigned to acute HIOP （without BDNF pre-treatment） and BDNF pre-treated groups, with 24 animals in each group. In the BDNF pre-treated group, the left eyes were intravitreally injected with 3 pg/kg BDNF 2 days prior to HIOP. Rats in the acute HIOP group were not pre-treated with BDNE HIOP models were established by increased intraocular pressure in the left eyes until the b-wave of flash electroretinogragh disappeared and pressure was maintained for 60 minutes. The right eyes of all rats were not treated and served as the normal controls. MAIN OUTCOME MEASURES： Retinal structure and cell numbers in the ganglion cell layer （GCL） were detected by Nissl staining; expression of trkB and phosphorylated trkB in the rat retina were determined by immunohistochemistry. RESULTS： A greater number of GCL neurons were observed in the pre-treated group compared to the acute HIOP group （P 〈 0.05）. TrkB expression was significantly increased following HIOP at days 1 and 3 （P 〈 0.05）, but expression varied between retinal areas. Although trkB expression decreased at 7 days, phosphorylated trkB dramatically decreased with increasing time （P 〈 0.05）. TrkB expression in BDNF pre-treated rats was simi

关键词： acute high intraocular pressure brain-derived neurotrophic factor tyrosine kinase receptor B phosphorylated trkB retina rats nerve factors neural regeneration

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Rapid construction of phosphatase and tensin homolog-deleted on chromosome ten gene recombinant adenovirus using the AdEasy system

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Neural Regeneration Research 2010年第15期5卷 1166-1170页

作者： Yongqiong Wei Lixue Chen Zhaofang Zeng Chongbiao Shen Key Laboratory of Laboratory Medical Diagnostics Ministry of Education Chongqing Medical University Chongqing 400016 China Chongqing Key Laboratory of Neurology First Affiliated Hospital Chongqing Medical University Chongqing 400016 China

Recent studies have shown that phosphatase and tensin homolog-deleted on chromosome ten （PTEN） gene plays an important role in ischemic brain damage and synaptic plasticity. The AdEasy system, which has been widely used, greatly simplifies preparation of recombinant adenovirus. Therefore, recombinant defective adenovirus vector carrying human PTEN tumor suppressor gene （Ad-PTEN） was constructed using the AdEasy-1 system and was transfected into HEK293 cells for packaging and amplification. Infection efficiency and expression intensity were observed in primary cultured rat hippocampal neurons infected with Ad-PTEN in vitro. Results revealed a cytopathic effect in green fluorescent protein expression, which increased with prolonged time. After three cycles of amplification, the adenovirus titer was increased to an adequate titer for infecting hippocampal neurons. The entire process typically requires 4-5 weeks for completion. Results suggested that recombinant defective adenovirus vector carrying the PTEN gene was successfully and rapidly constructed using the AdEasy system.

关键词： phosphatase and tensin homolog-deleted on chromosome ten recombinant adenovirus AdEasy system vector construction nerve factors neural regeneration

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