检索结果-南通市图书馆

Residue substitution enhances the immunogenicity of neoepitopes from gastric cancers

Cancer Biology & Medicine 2021年第4期18卷 1053-1065页

作者： Huahui Yu Jieyu Li Yuan Yuan Yu Chen Jingwen Hong Chunmei Ye Wansong Lin Huijing Chen Zengqing Guo Bo Li Yunbin Ye The School of Basic Medical Sciences Fujian Medical UniversityFuzhou 350122China Laboratory of Immuno-Oncology Fujian Cancer Hospital&Fujian Medical University Cancer HospitalFuzhou 350014China Fujian Key Laboratory of Translational Cancer Medicine Fuzhou 350014China BGI-Shenzhen Shenzhen 518083China BGI Education Center University of Chinese Academy of SciencesShenzhen 518083China Department of Oncology Fujian Cancer Hospital&Fujian Medical University Cancer HospitalFuzhou 350014China

Objective:Neoantigens arising from gene mutations in tumors can induce specific immune responses,and neoantigen-based immunotherapies have been tested in clinical ***,we characterized the efficacy of altered neoepitopes in improving immunogenicity against gastric ***:Raw data of whole-exome sequencing derived from a patient with gastric cancer were analyzed using bioinformatics methods to identify *** were modified by P1Y(the first amino acid was replaced by tyrosine)and P2L(the second amino acid was replaced by leucine).T2 binding and stability assays were used to detect the affinities between the neoepitopes and the HLA molecules,as well as the stabilities of *** cells(DCs)presented with neoepitopes stimulated naïve CD8+T cells to induce specific cytotoxic T *** and carboxyfluorescein succinimidyl ester were used to detect IFN-γand TNF-αlevels,and T cell *** was detected by flow *** cytotoxicity of T cells was determined using the lactate dehydrogenase ***:Bioinformatics analysis,T2 binding,and stability assays indicated that residue substitution increased the affinity between neoepitopes and HLA molecules,as well as the stabilities of *** presented with altered neoepitopes stimulated CD8+T cells to release more IFN-γand had a greater effect on promoting proliferation than wild-type ***8+T cells stimulated with altered neoepitopes killed more wild-type neoepitope-pulsed T2 cells than those stimulated with wild-type neoepitopes,by secreting more IFN-γ,TNF-α,and ***:Altered neoepitopes exhibited greater immunogenicity than wild-type *** substitution could be used as a new strategy for immunotherapy to target neoantigens.

关键词： Gastric cancer bioinformatics neoepitope residue substitution immunotherapy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：