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negative cross-resistance of a pyrethroid-resistant Drosophila mutant to Phryma leptostachya-derived haedoxan A

Insect Science 2022年第3期29卷 817-826页

作者： Xingtao Qie Yuzhe Du Ahmed A.A.Aioub Ke Dong Zhaonong Hu Institution of Pesticide Science College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi 712100China Key Laboratory of Botanical Pesticide R&D in Shaanxi Province YanglingShaanxi 712100China Southern Insect Management Research Unit Agriculture Research ServiceUnited States Department of Agriculture141 Experiment Station RoadStonevilleMS 38776USA Plant Protection Department Faculty of AgricultureZagazig UniversityZagazig44511 Egypt Department of Biology Duke UniversityDurhamNC 27708USA

Voltage-gated sodium channels are the primary target of pyrethroid insecticides.Mutations in sodium channel confer knockdown resistance(kdr)to pyrethroids in various arthropod pests.Haedoxan A(HA)is the major insecticidal component from Phryma leptostachya.It has been shown that HA alters electrical responses at the Drosophila neuromuscular junction and modifies the gating properties of cockroach sodium channels expressed in Xenopus oocytes.However,whether sodium channel mutations that confer pyrethroid resistance also affect the action of HA is unknown.In this study,we conducted bioassays using HA and permethrin in two Drosophila melanogaster strains:w^(1118),an insecticide-susceptible strain,and para^(tsl),a pyrethroid-resistant strain due to a I265N mutation in the sodium channel,and identified a new case of negative cross-resistance(NCR)between permethrin and HA.Both para^(tsl) larvae and adults were more resistant to permethrin,as expected.However,both para^(tsl) larvae and adults were more sensitive to HA compared to w^(1118).We confirmed that the I265N mutation reduced the sensitivity to permethrin of a Drosophila sodium channel variant,DmNa_(v)22,expressed in Xenopus oocytes.Interestingly,the I265N mutation also abolished the effect of HA on sodium channels.Further characterization showed that I265 on the sodium channels is critical for the action of both pyrethroids and HA on sodium channels,pointing to an overlapping mode of action between pyrethroids and HA on the sodium channel.Overall,our results suggest an I265N-independnt mechanism(s)in para^(tsl) flies that is responsible for the NCR between permethrin and HA at the whole insect level.

关键词： botanical insecticide haedoxan A insecticidal mechanism negative crossresistance permethrin voltage-gated sodium channel

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