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p42/p44 mitogen-activated protein kinases inhibit atrial natriuretic peptide mRNA transcription in gp130-mediated hypertrophic ventricular myocytes

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Asian Pacific Journal of Tropical Medicine 2014年第3期7卷 216-220页

作者： Zhan-Ling Dong Yang Wang Tian-Fa Li Shao-Jiang Zheng Yue-Qiong Kong You-Ling Lan Jun-Li Guo Shi-Gan Fu Department of Physiology and Cardiovascular Institute Hainan Medical College Department of Physiology Hainan Provincial Health School

Objective:To understand the role of ANP mRNA transcription regulation in gpl30-mediated cardiomyocyte hypertrophy,and the involved mitogen-aetivated protein kinase kinase(MEK)-extracellular signal-regulated kinase(ERK,also called p42/p44 MAPK)signaling ***:isolated neonatal ventricular myocytes were treated with different concentrations of CT-1(10^(-9),10^(-8)and 10^(-7)mol/L).MTT was used to analyze the viability and RT-PCR was used to detect ANP mRNA levels in *** inhibit p42/p44 MAPK activity in hypertrophic cardiomyoeytes,the cells were pretreated with a specific MEKI ***:CT-1significantly induced ANP mRNA expression and the viability of canliomyocytes in a doseand time-dependent ***,blocking p42/p44 MAPK activity by the special MEk1 inhibitor uprcgulatcd the ANP ***:p42/p44 MAPK have an important role in suppressing ANP mRNA transcription and cell activity in gpl30-mediated hypertrophic ventricular myocytes.

关键词： Cardiomyocytes Hypertrophy Atrial natriuretic peptide

STUDY ON MULTI-POINT SITE-SPECIFIC MUTAGENESIS——DOUBLE-POINT SITE-SPECIFIC MUTAGENESIS FOR MODIFYING α-HUMAN ATRIAL natriuretic PEPTIDE GENE

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Chinese Science Bulletin 1990年第3期35卷 232-238页

作者：杨迪谢毅施文闵永洁王启松 Institute of Genetics Fudan University Shanghai 200433 PRC

Although oligonucleotide-directed mutagenesis has been widely applied in the field of molecular biology, this method has some disadvantages: It is time-consuming and overelaborate, besides only a single base mutation ... 详细信息

关键词： site-specific mutagenesis double-point site-specific mutagenesis α-human atrial natriuretic peptide.

Effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in rats

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Asian Pacific Journal of Tropical Medicine 2014年第5期7卷 398-401页

作者： Yan Xu Yu Yang Ying-Quan Luo Department of Geratology Second Xiangya HospitalCentral South University

Objective:To investigate the effect of atorvastatin on serum oxidative stress and N-terminal brain natriuretic peptide expression in ***:A total of 40 healthy male SD rats were randomly divided into the sham group(Croup A,n=10,saline 5 mL/d),ischemia-reperfusion group(Group B,n=10,saline S mL/d),atorvastatin group(Group C,n=*** 20 mg/kg·d),atorvastatin + N-amino-arginine group(Group D,n=10,atorvastatin 20 mg/kg·d + N-amino arginine 15 mg/kg).Myocardial ischemia-reperfusion rat model was eslablished after 3 days of gavage.N-amino arginine 15 mg/kg was given by tail vein injection 15 min before *** reperfusion,enzymology indicators such us creatine kinase(CK) and lactate dehydrogenase and the oxidative stress parameters such as nitric oxide(NO),malondialdehyde(MDA) and total superoxide dismutase(TSOD),and n-terminal pro-brain natriuretic peptide(NT-proBNP)expression was detected by ***:LDH and CK levels of group A were significantly lower than the outer three groups,and group B was the *** was significant difference between group B and group C(P0.05).MDA levels in group B were significantly higher than the other three *** lowest was group A,followed by group C,the difference among groups was significantly(P<0.05).TSOD and NO levels in group B was the lowest,the level in group A was the highest,followed by group C,the difference among groups was significant(P<0.05).NT-proBNP level in group B was significantly higher than the other three groups,the lowest was group A,followed by group C,the difference among groups was significant(P<0.05).Conclusions:Atorvastatin has a protective effect on the myocardial injury in the myocardial ischemia and reperfusion *** can increase NO synthesis and decrease MDA content,increase serum TSOD activity and the oxidative stress effect,meanwhile protect myocardial cells and reduce myocardial injur

关键词： Atorvastatin Myocardial ischemia-reperfusion Oxidative stress n-terminal pro-brain natriuretic peptide

Plasma N-terminal pro-brain natriuretic peptide levels in elderly patients with isolated diastolic dysfunction

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Journal of Geriatric Cardiology 2005年第4期2卷 211-215页

作者： Yixin SONG Qing LIN Xiaomin SHI Yunyun QI Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China Department of Geriatric Medicine First Hospital of Beijing University Beijing 100034 China

Objective To investigate plasma N-terminal pro-brain natriuretic peptide (NT-BNP) levels and to assess their clinical significance in elderly patients with isolated diastolic dysfunction. Methods Plasma NT-BNP level were measured by electrochemiluminescence immunoassay in 34 symptomatic patients (Group 1), 34 asymptomatic patients (Group 2) with isolated diastolic dysfunction, and in 16 elderly healthy subjects (control group, Group 3), serving controls. Colored Doppler echocardiography was performed to evaluate the patients' cardiac structures and functions. Results The plasma NT-BNP level in Group 1 was significantly higher than those in Group 2 and Group 3 and increased with the severity of heart failure. There was no significant difference of plasma NT-BNP levels between Group 2 and Group 3 (p>0.05). A NT-BNP value of 102.75 pg/mL showed a sensitivity of 88.2%, a specificity of 87.5%, and an accuracy of 88.1% for diagnosing diastolic dysfunction. Patients with restrictive filling pattern on echocardiography had higher NT-BNP levels than those of impaired relaxation pattern (1961.2±304.9 versus 460.1±92.7pg/mL, p<0.001). Conclusion The elevation of plasma NT-BNP level in elderly patients with isolated diastolic dysfunction correlates with the severity of their diastolic abnormalities. The level of plasma NT-BNP has an important clinical value in the diagnosis of elderly patients with isolated diastolic dysfunction.

关键词： elderly isolated diastolic dysfunction N-terminal pro-brain natriuretic peptide echocardiography

Growth differentiation factor-15 combined with N-terminal prohormone of brain natriuretic peptide increase 1-year prognosis prediction value for patients with acute heart failure: a prospective cohort study

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Chinese Medical Journal 2019年第19期132卷 2278-2285页

作者： Ji Hao Iokfai Cheang Li Zhang Kai Wang Hui-Min Wang Qian-Yun Wu Yan-Li Zhou Fang Zhou Dong-Jie Xu Hai-Feng Zhang Wen-Ming Yao Xin-Li Li Department of Cardiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Cardiology The Second Affiliated Hospital of Xuzhou Medical CollegeXuzhouJiangsu 221002China

Background:Clinical assessment and treatment guidance for heart failure depends on a variety of *** objective of this study was to investigate the prognostic predictive value of growth differentiation factor-15(GDF-15)and N-terminal prohormone of brain natriuretic peptide(NT-proBNP)in assessing hospitalized patients with acute heart failure(AHF).Methods:In total,260 patients who were admitted for AHF in the First Affiliated Hospital of Nanjing Medical University were enrolled from April 2012 to May *** history and blood samples were collected within 24 h after the *** primary endpoint was the all-cause mortality within 1 *** patients were divided into survival group and death group based on the *** established mortality risk factors and serum GDF-15 level,receiver-operator characteristic(ROC)analyses were *** regression analyses were used to further analyze the combination values of NT-proBNP and ***:Baseline GDF-15 and NT-proBNP were significantly higher amongst deceased than those in survivors(P<0.001).In ROC analyses,area under curve(AUC)for GDF-15 to predict 1-year mortality was 0.707(95%confidence interval[CI]:0.648–0.762,P<0.001),and for NT-proBNP was 0.682(95%CI:0.622–0.738,P<0.001).No statistically significant difference was found between the two markers(P=0.650).Based on the optimal cut-offs(GDF-15:4526.0 ng/L;NT-proBNP:1978.0 ng/L),the combination of GDF-15 and NT-proBNP increased AUC for 1-year mortality prediction(AUC=0.743,95%CI:0.685–0.795,P<0.001).Conclusions:GDF-15,as a prognostic marker in patients with AHF,is not inferior to *** the two markers could provide an early recognition of high-risk patients and improve the prediction values of AHF long-term *** trial registration:ChiCTR-ONC-12001944,http://***.

关键词： Growth differentiation factor-15 Heart failure N-terminal pro-B type natriuretic peptide Prognosis

Physiology of natriuretic peptides: The volume overload hypothesis revisited

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World Journal of Cardiology 2014年第1期6卷 4-7页

作者： Olli Arjamaa Department of Biology University of Turku

The discovery of the natriuretic peptide system in the early 1980s aroused great interest among clinical cardiologists. The heart was not a mechanical pump alone, but also an endocrine organ that had powerful effects on blood circulation. natriuretic peptides caused both natriuresis and diuresis, and they responded to a volume overload which caused either stretch or pressure on the heart. As a result, the findings led to the conclusion that the human body had a hormone with effects similar to those of a drug which treats high blood pressure. Later, it became evident that the volume contraction was fortified by extrarenal plasma shift. Here, a hypothesis is presented in which the role of natriuretic peptides is to regulate oxygen transport as the volume contraction leads to hemoconcentration with an increased oxygen-carrying capacity. Wall stress, either chemical or mechanical, changes the oxygen gradient of the myocardium and affects the diffusion of oxygen within a myocyte. In support of this hypothesis, hypoxia-response elements have been found in both the atrial natriuretic peptide and the brain natriuretic peptide genes.

关键词： natriuretic peptides Hypoxia Hemoglobin concentration Volume overload

N-terminal pro-B-type natriuretic peptide levels after hospital discharge in patients with acute myocardial infarction for predicting long term outcome

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South China Journal of Cardiology 2019年第3期20卷 168-173页

作者： ZHENG Bao-juan CHEN Jian-jia MA dun-liang ZHUO Sheng-qing TIAN Xiang-ting Department of Cardiology Guangdong Cardiovascu lar InstituteGuangdong Academy of Medical ScienceGuangdong Provincial People's HospitalGuangzhou 510100 De partment of Cardiology Lecong HospitalFoshan 528315 Department of Cardiology Guangdong General Hospital's Nanhai HospitalFoshan 528200China

Background N-terminal-pro-brain natriuretic peptide(NT-pro-BNP)is associated with worse outcome in patients with acute myocardial infarction(AMI). However,the role of short-term follow-up of NT-pro-BNP level remains unclear. Methods Three hundred and sixty-two patients diagnosed with AMI were retrospectively enrolled in this study from March 2014 to March 2017 in our center. Blood samples were obtained at initial admission and again within 1 month after hospital discharge. The univariate and multivariate cox regression analyses including significant covariables were performed on NT-pro-BNP level at admission,discharge,or change from admission to discharge to predict adverse cardiovascular events(MACE)as study endpoints. Results There were 211 cases in NT-pro-BNP decrease group,while 151 cases in NT-pro-BNP increase group. The median follow-up was 365 days(interquartile range[IQR],322-861 days). After adjusting the covariables in the multiple logistic regression analysis,follow-up NT-pro-BNP level was still a significant independent predictor for MACE(OR,1.395;95% CI,1.102-1.869,P=0.005). However,the initial NT-pro-BNP level or change of NT-pro-BNP level had no significant predictive value for MACE. Conclusions A short-term follow-up NT-pro-BNP level after hospital discharge is a powerful prognostic biomarker for MACE in patients with AMI.[S Chin J Cardiol 2019;20(3):168-173]

关键词： N-terminal pro-B-type natriuretic peptide major adverse cardiovascular events prognosis

Forskolin Modulates the Inhibitory Effect of C-Type natriuretic Peptide on Hypoxia-Induced Atrial Dynamics and Hypoxia Inducible Factor 1 Alpha Activity

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Journal of Biosciences and Medicines 2017年第1期5卷 1-10页

作者： Chengming Guan Yanan Jia Chaochao Bian Bo Zhang Dazhi Ding Xun Cui Institue of Clinical Medicine Yanbian University Yanji China Department of Physiology School of Medicine Yanbian University Yanji China Key Laboratory of Organism Functional Factors of the Changbai Mountain Ministry of Education Yanbian University Yanji China Cellular Function Research Center Yanbian University Yanji China

Our study investigated effects of C-type natriuretic peptide (CNP) on atrial dynamics and hypoxia inducible factor 1 alpha (HIF-1α) activity in perfused beating rat atria, under hypoxic conditions. Hypoxia significantly increased the levels of HIF-1α, concomitant with decreased trial dynamics. CNP (0.1 μmol/L) further decreased atrial dynamics under hypoxia and suppressed hypoxia-induced stimulation of HIF-1α expression. An adenylylcyclase (AC) activator, forskolin (0.1 μmol/L), significantly up-regulated atrial phosphodiesterase subtype 3A (PDE 3A) protein without affecting hypoxia-induced dynamics. In the presence of forskolin, the inhibitory effects of CNP on hypoxia-induced atrial dynamics and HIF-1α levels were significantly attenuated. Forskolin also prevented hypoxia-induced downregulation of PDE3A protein. These findings suggested that CNP inhibited atrial dynamics and HIF-1α activity in the isolated perfused beating rat atria under hypoxic conditions. Furthermore, both effects were modulated by the AC activator forskolin, through activation of CNP-PDE 3A signaling.

关键词： C-Type natriuretic Peptide Hypoxia Inducible Factor-1α Phosphodiesterase Adenylyl Cyclase Forskolin

The role of B-type natriuretic peptide in the evaluation of congestive heart failure patients in emergency department

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Open Journal of Clinical Diagnostics 2012年第2期2卷 11-17页

作者： Hao Wang Tiffany Littleton Sonya J. Wilson Ferran Ros Richard D. Robinson Kathleen A. Delaney Department of Emergency Medicine JPS Health Network Fort Worth USA JPS Health Network

Aim: Congestive heart failure (CHF) is a severe cardiovascular disorder seen in the Emergency Department (ED). B-type natriuretic Peptide (BNP) is usually ordered to evaluate the CHF severity. However, it is difficult to interpret serum BNP level when different clinical entities existed. The aim of this study is to illustrate the correlation between serum BNP level and relevant clinical variables and further determine the role of serum BNP in different CHF patients. Methods: Univariate comparisons between 26 clinical variables and serum BNP level were analyzed. In order to avoid confounding factors, potential independent clinical variables were analyzed together using multivariate regression. Results: 529 CHF patients were reviewed and divided into different groups by 26 clinical variables. Serum BNP levels were found statistically significant different by univariate compareson between groups divided by 8 clinical variables that included obesity, diastolic/systolic heart failure (HF), serum blood urea nitrogen (BUN) level, serum creatinine (Cr) level, serum sodium (Na) level, patients taking loop diuretics, history of cerebrovascular accident (CVA), and history of dementia. Among all 8 clinical variables, obesity, serum BUN, Cr level, and diastolic/systolic HF had weak-to-moderate correlation effects with serum BNP level by correlation coefficient analysis. However, only obesity and dia- stolic/systolic HF were two moderately stronger clini- cal variables that can affect the serum BNP levels by multivariate regression. Analyzing CHF patients separately by obesity and diastolic/systolic HF subsets showed longer hospitalization in diastolic HF patients with relatively higher serum Cr level. In addition, poor correlation was found between serum BNP level and length of hospitalization (LOH) as well. Conclusion: High variability of serum BNP levels exists in CHF patients with weak-to-moderate correlation effects particularly on obesity and diastolic/systolic *** is rec

关键词： Congestive Heart Failure B-Type natriuretic Peptide Clinical Variables Emergency Department