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Characterization and Fine Mapping of a Novel Rice narrow leaf Mutant nal9

Journal of Integrative Plant Biology 2013年第11期55卷 1016-1025页

作者： Wei Li Chao Wu Guocheng Hu Li Xing Wenjing Qian Huamin Si Zongxiu Sun Xingchun Wang Yaping Fu Wenzhen Liu State Key Laboratory of Rice Biology China National Rice Research Institute College of Life Sciences Shanxi Agricultural University Institute of Horticulture Zhejiang Academy of Agricultural Sciences Biotechnology Research Center China Three Gorges University

A narrow leaf mutant was isolated from transgenic rice （Oryza sativa L.） lines carrying a T-DNA insertion. The mutant is characterized by narrow leaves during its whole growth period, and was named nal9 （narrow leaf 9）. The mutant also has other phenotypes, such as light green leaves at the seedling stage, reduced plant height, a small panicle and increased tillering. Genetic analysis revealed that the mutation is controlled by a single recessive gene. A hygromycin resistance assay showed that the mutation was not caused by T-DNA insertion, so a map-based cloning strategy was employed to isolate the nal9 gene. The mutant individuals from the F2 generations of a cross between the nal9mutant and Longtepu were used for mapping. With 24 F2 mutants, the nal9 gene was preliminarily mapped near the marker RM156 on the chromosome 3. New INDEL markers were then designed based on the sequence differences between japonica and indica at the region near RM156. The nal9 gene was finally located in a 69.3 kb region between the markers V239B and V239G within BAC OJ1212_C05 by chromosome walking. Sequence and expression analysis showed that an ATP-dependent CIp protease proteolytic subunit gene （CIpP） was most likely to be the nal9 gene. Furthermore, the nal9 mutation was rescued by transformation of the CIpP cDNA driven by the 35S promoter. Accordingly, the CIpP gene was identified as the NAL9 gene. Our results provide a basis for functional studies of NAL9 in future work.

关键词： ice narrow leaf mutant nal9 fine mapping.

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Identification and gene mapping of a narrow and upper-albino leaf mutant in rice(Oryza sativa L.)

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Science Bulletin 2012年第Z2期 3798-3803页

作者： WANG Feng,TANG YanQiang,MIAO RunLong,XU FangFang,LIN TingTing,HE GuangHua & SANG XianChun * Rice Research Institute,Chongqing Key Laboratory of Application and Safety Control of Genetically Modified Crops,Engineering Research Center of South Upland Agriculture,Ministry of Education,Southwest University,Chongqing 400716,China

The leaf blade consists of color and shape *** of leaf-blade development are important for improvement of rice yield and quality because it is an essential organ for photosynthesis.A narrow and upper-albino leaf mutant(nul1) was identified from among progeny of the indica restorer line Jinhui10 raised from seeds treated with ethyl methane *** field conditions,the mutant displayed narrow and upper-albino leaf blades with significantly decreased photosynthetic pigment contents throughout their *** narrow-leaf trait is caused by a decreased number of small *** contrast to the wild type,the growth period was extended by approximately 8 d and agronomic traits,such as effective panicle number,percentage seed set and 1000-grain weight,declined significantly in the nul1 *** analysis suggested that the narrow and upper-albino leaf characteristics showed coseparation and were controlled by one recessive *** Nul1 gene was mapped onto chromosome 7 between the Indel marker Ind07-1 and the Simple Sequence Repeat marker *** physical distance between the markers was 75 kb and eight genes were annotated in this region based on the rice Nipponbare genome *** results provide a foundation for cloning and function analysis of Nul1.

关键词： rice Oryza sativa narrow leaf albino gene mapping

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