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The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

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Frontiers of Materials Science 2016年第4期10卷 346-357页

作者： Xuran GUO Kaile ZHANG Mohamed EL-AASSAR Nanping WANG Hany EL-HAMSHARY Mohamed EL-NEWEHY Qiang FU Xiumei MO State Key Lab for Modification of Chemical Fibers & Polymer Materials College of Chemistry & Chemical Engineering and Biotechnology Donghua University Shanghai 201620 China Department of Urology Affiliated Sixth People's Hospital Shanghai Jiaotong University Shanghai 200030 China Polymer Materials Research Department Advanced Technology and New Material Institute City of Scientific Research and Technological Applications (SRTA-City) New Borg El-Arab City Alexandria 21934 Egypt Shanghai Aquatic Product Research Institute Shanghai 200030 China Department of Chemistry College of Science King Saud University Riyadh i 1451 Saudi Arabia Department of Chemistry Faculty of Science Tanta University Tanta 31527 Egypt Shandong International Biotechnology Park Development Co. Ltd. Shanghai 200030 China

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagenlpoly（L-lactide-co-caprolactone）（ColIP（LLA-CL）） nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively, in addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wntsignaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H＆E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning （DLY）. It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.

关键词： biomaterials nanoyarn electrospinning inhibitor

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Fabrication of a Bi-layer Tubular Scaffold Consisted of a Dense Nanofibrous Inner Layer and a Porous nanoyarn Outer Layer for Vascular Tissue Engineering

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Journal of Donghua University(English Edition) 2014年第5期31卷 718-722页

作者： CHEN Jian-feng LIU Wei WU Tong LI Da-wei ZHANG Jian-guang WANG Nan-ping MO Xiu-mei State KeyLaboratory for Modifications of Chemical Fibers and Polymer Materials- Coilege of Material Science and EngineeringDonghua University Shanghai 201620 China Biomaterials and Tissue Engineering Laboratory College of Chemistry Chemical Engineering and Biotechnology DonghuaUniversity Shanghai 201620 China Shanghai Fisheries Research Institute Shanghai 201620 China

Recent years, it has attracted more attentions to increase the porosity and pore size of nanofibrous scaffolds to provide the for the cells to grow into the small-diameter vascular grafts. In this study, a novel bi-layer tubular scaffold with an inner layer and an outer layer was fabricated. The inner layer was random collagen/poly （ L-lactide-co-caprolactone ） I P （ LLA- CL） ] nanofibrous mat fabricated by conventional electrospinning and the outer layer was aligned collagen/P （LLA-CL） nanoyarns prepared by a dynamic liquid dectrospinning method. Fourier transform infrared spectroscopy （FTIR） was used to characterize the chemical structure. Scanning electron microscopy （ SEM ） was employed to observe the morphology of the layers and the cross- sectioned bi-layer tubular scaffold. A liquid displacement method was employed to measure the porosities of the inner and outer layers. Stress-strain curves were obtained to evaluate the mechanical properties of the two different layers and the bi-layer membrane. The diameters of the nanofibers and the nanoyarns were （480 ± 197 ） nm and （ 19.66 ± 4.05 ） μm, respectively. The outer layer had a significantly higher porosity and a larger pore size than those of the inner layer. Furthermore, the bi-layer membrane showed a good mechanical property which was suitable as small-diameter vascular graft. The results indicated that the bi-layer tubular scaffold had a great potential application in small vascular tissue engineering.

关键词： nanoyarn poly ( L-lactide-co-caprolactone ) [ P ( LLA-CL ) ] bi-layer tubular scaffold porous structure small vascular tissueengineering

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Double‑Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration

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Advanced Fiber Materials 2023年第2期5卷 662-680页

作者： Kaile Zhang Muhammad Aqeel Bhutto Liyang Wang Kai Wang Jie Liu Wenyao Li Wenguo Cui Qiang Fu The Department of Urology Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai Jiao Tong UniversityShanghai 200233People’s Republic of China Institute of Biotechnology and Genetic Engineering University of SindhJamshoro 76080Pakistan School of Materials Science and Engineering Shanghai University of Engineering ScienceShanghai 201620People’s Republic of China Clinical Research Center Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghai 200233People’s Republic of China Regenerative Medicine Research Center The First People’s Hospital of Yunnan ProvinceThe Affiliated Hospital of Kunming University of Science and TechnologyKunming 650000People’s Republic of China Shanghai Institute of Traumatology and Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint DiseasesRuijin HospitalShanghai Jiao Tong University School of MedicineShanghai 200025People’s Republic of China

Insufficient bionic performance is a structural obstacle and makes urethral repair unobtainable.To overcome this challenge,we mimicked the urethral matrix and applied two electrospinning techniques to build a double-layer sponge tube of nanofib-ers and nanoyarns.Intriguingly,silk fibroin(SF)and vitamin B5(VitB5)could be introduced to increase the elasticity of the outer layer and reduce the hydrophobicity to further improve mesenchymal cell proliferation.Systematic experiments validated the bionic structure,biocompatibility,and exosome delivery capacity in this scaffold.We achieved scarless urethral repair by delivering the bioactive growth factors from adipose-derived stem cell exosomes by physical absorption.Biological regeneration of the urethra can be accomplished with continuous epithelium in animals.Furthermore,bioinformatics studies revealed that the expression of cell proliferation and fibrotic genes(e.g.,Wnt7a,cfa-miR-574)was responsible for the bio-logical regeneration of the adipose-derived stem cells exosomes(ADSC-exos)by delivering poly l-lactide-co-caprolactone/SF/VitB5 bilayer sponge(PSVBS)via reduced fibrosis gene expression,as well as improved epithelial formation and blood vessel formation.Therefore,the PSVBS design appeared to be an instructive approach for urethral and other tubular organ regeneration.

关键词： Electrospinning Nanofiber nanoyarn Urethral stricture Urethral reconstruction

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