检索结果-南通市图书馆

Tailoring RGD local surface density at the nanoscale toward adult stem cell chondrogenic commitment

Nano Research 2017年第6期10卷 1959-1971页

作者： Anna Lagunas Iro Tsintzou Yolanda Vida Daniel Collado Ezequiel Perez-Inestrosa Cristina Rodriguez Pereira Joana Magalhaes Jose A. Andrades Josep Samitier Networking Biomedical Research Center (CIBER) C/Monforte de Lemos 3-5 Pabellon 11 Planta 0 Madrid 28029 Spain Institute for Bioengineering of Catalonia (IBEC) Baldiri-Reixac 10-12 Barcelona 08028 Spain Instituto de Investigacion Biomedica de Malaga (IBIMA) Department of Organic Chemistry Universidad de Malaga (UMA) Malaga 29071 Spain Andalusian Centre for Nanomedicine and Biotechnology-BlONAND Parque Tecnoldgico de Andalucia Mdlaga 29590 Spain Unidad de Bioingenieria Tisulary Terapia Celular (GBTTC-CHUAC) Grupo de Reumatologia Instituto de Investigacion Biomedica de A Coru6a (INIBIC) Complexo Hospitalario Universitario de A Coruf~a (CHUAC) Sergas Universidade da Coruna (UDC) As Xubias 84 Coruna 15006 A Spain Cell Biology Genetics and Physiology Department University de Malaga (UMA) Campus Teatinos Malaga 29071 Spain Department of Engineering Electronics University of Barcelona (UB) Marti i Franques 1-11 Barcelona 08028 Spain

Arginine-glycine-aspartic acid （RGD） dendrimer-based nanopatterns on poly（L- lactic acid） were used as bioactive substrates to evaluate the impact of the RGD local surface density on the chondrogenic induction of adult human mesenchymal stem cells. During chondrogenic commitment, active extracellular matrix （ECM） remodeling takes place, playing an instructive role in the differentiation process. Although three-dimensional environments such as pellet or micromass cultures are commonly used for in vitro chondrogenic differentiation, these cultures are rather limited with respect to their ability to interrogate cells in celI-ECM interactions. In the present study, the nanopatterns of the tunable RGD surface density were obtained as a function of the initial dendrimer concentration. The local RGD surface density was quantified through probability contour plots for the minimum interparticle distance, constructed from the corresponding atomic force microscopy images, and correlated with the cell adhesion and differentiation response. The results revealed that the local RGD surface density at the nanoscale acts as a regulator of chondrogenic commitment, and that intermediate adhesiveness of cells to the substrates favors mesenchymal cell condensation and early chondrogenic differentiation.

关键词： dendrimer arginine-glycine-asparticacid (RGD) nanopattem human mesenchymalstem cells (hMSCs) chondrogenesis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：