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Research Article Dynamically crosslinked nanocapsules for the efficient and serumresistant cytosolic protein delivery

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Nano Research 2024年第3期17卷 1760-1771页

作者： Qiang Yang Ningyu Liu Ziyin Zhao Xun Liu Lichen Yin Institute of Functional Nano and Soft Materials(FUNSOM) Jiangsu Key Laboratory of Carbon-Based Functional Materials and DevicesSoochow UniversitySuzhou 215123China Department of Thoracic Surgery The Second Affiliated Hospital of Soochow UniversitySuzhou 215004China

Intracellular protein delivery is critical to the development of protein-based biopharmaceuticals and therapies.However,current delivery vectors often suffer from complicated syntheses,low generality among various proteins,and insufficient serum stability.Herein,we developed an enlightened cytosolic protein delivery strategy by dynamically crosslinking epigallocatechin gallate(EGCG),low-molecular-weight polyethylenimine(PEI 1.8k),and 2-acetylphenylboric acid(2-APBA)on the protein surface,hence forming the EPP-protein nanocapsules(NCs).EGCG enhanced protein encapsulation via hydrogen bonding,and reduced the positive charge density of PEI to endow the NCs with high serum tolerance,thereby enabling effective cellular internalization in serum.The formation of reversible imine and boronate ester among 2-APBA,EGCG,and PEI 1.8k allowed acid-triggered dissociation of EPP-protein NCs in the endolysosomes,which triggered efficient intracellular release of the native proteins.Such strategy therefore showed high efficiency and universality for diversities of proteins with different molecular weights and isoelectric points,including enzyme,toxin,antibody,and CRISPR(clustered regularly interspaced short palindromic repeats)-Cas9 ribonucleoprotein(RNP),outperforming the commercial protein transduction reagent PULSin and RNP transfection reagent lipofectamine CMAX.Moreover,intravenously(i.v.)injected EPP-saporin NCs efficiently delivered saporin into 4T1 tumor cells to provoke robust antitumor effect.This simple,versatile,and robust cytosolic protein delivery system holds translational potentials for the development of protein-based therapeutics.

关键词： nanocapsules dynamic crosslinking cytosolic protein delivery serum resistance antitumor therapy genome editing

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Facile synthesis of chromium chloride/poly(methyl methacrylate) core/shell nanocapsules by inverse miniemulsion evaporation method and application as delayed crosslinker in secondary oil recovery

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Petroleum Science 2023年第1期20卷 396-406页

作者： Jing-Yang Pu Keith P.Johnston Ping-Keng Wu Muaaz Ahmad Ming-Liang Luo Na Zhang Ju-Tao He School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Key Laboratory of Unconventional Oil&Gas Development(China University of Petroleum(East China)) Ministry of EducationQingdao266580ShandongChina Mcketta Department of Chemical Engineering The University of Texas at AustinAustinTX78712USA Department of Energy and Mining Engineering Shandong University of Science and TechnologyQingdao266590ShandongChina No.7 Oil Production Plant of PetroChina Changqing Oilfield Branch Qingyang745100GansuChina

Cr(III)ehydrolyzed polyacrylamide(HPAM)gels have been extensively studied as a promising strategy controlling waste water production for mature oilfields.However,the gelation time of the current technologies is not long enough for in-depth placement.In this study,we report a novel synthesis method to obtain chromium chloride/poly(methyl methacrylate)(PMMA)nanocapsules which can significantly delay the gelation of HPAM through encapsulating the chromium chloride crosslinker.The chromium chloride-loaded nanocapsules(CreNC)are prepared via a facile inverse miniemulsion evaporation method during which the hydrophobic PMMA polymers,pre-dispersed in an organic solvent,were carefully controlled to precipitate onto stable aqueous miniemulsion droplets.The stable aqueous nanodroplets(W)containing Cr(III)are dispersed in a mixture of organic solvent(O1)with PMMA and nonsolvent medium(O2)to prepare an inverse miniemulsion.With the evaporation of the O1,PMMA forms CreNCs around the aqueous droplets.The CreNCs are readily transferred into water from the organic nonsolvent phase.The CreNCs exhibit the tunable size(358-983 nm),Cr loading(7.1%-19.1%),and Cr entrapment efficiency(11.7%-80.2%),with tunable zeta potentials in different PVA solutions.The CreNCs can delay release of Cr(III)and prolong the gelation time of HPAM up to 27 days.

关键词： nanocapsules Inverse miniemulsion evaporation Chromium chloride crosslinker HPAM gelation Secondary oil recovery

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Sustained‑Release nanocapsules Enable Long‑Lasting Stabilization of Li Anode for Practical Li‑Metal Batteries

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Nano-Micro Letters 2020年第12期12卷 239-250页

作者： Qianqian Liu Yifei Xu Jianghao Wang Bo Zhao Zijian Li Hao Bin Wu School of Materials Science and Engineering Zhejiang UniversityHangzhou 310027People’s Republic of China

A robust solid-electrolyte interphase(SEI)enabled by electrolyte additive is a promising approach to stabilize Li anode and improve Li cycling efficiency.However,the self-sacrificial nature of SEI forming additives limits their capability to stabilize Li anode for long-term cycling.Herein,we demonstrate nanocapsules made from metal–organic frameworks for sustained release of LiNO3 as surface passivation additive in commercial carbonate-based electrolyte.The nanocapsules can offer over 10 times more LiNO3 than the solubility of LiNO3.Continuous supply of LiNO3 by nanocapsules forms a nitride-rich SEI layer on Li anode and persistently remedies SEI during prolonged cycling.As a result,lifespan of thin Li anode in 50μm,which experiences drastic volume change and repeated SEI formation during cycling,has been notably improved.By pairing with an industry-level thick LiCoO2 cathode,practical Li-metal full cell demonstrates a remarkable capacity retention of 90%after 240 cycles,in contrast to fast capacity drop after 60 cycles in LiNO3 saturated electrolyte.

关键词： Metal–organic frameworks LiNO3 nanocapsules Lithium-metal anode Lithium-metal batteries

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Effect of 15-Deoxy-A^12,14-prostaglandin J2 nanocapsules on Inflammation and Bone Regeneration in a Rat Bone Defect Model

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Chinese Medical Journal 2017年第3期130卷 347-356页

作者： Qi Tang Li-Li Chen Fen Wei Wei-Lian Sun Li-Hong Lei Pei-Hui Ding Jing-Yi Tan Xiao-Tao Chen Yan-Min Wu Department of Oral Medicine The Second Affiliated Hospital of School of Medicine of Zhejiang University Hangzhou Zhejiang 310009 China

Background： 15-Deoxy-^△^12、14-prostaglandin J2 （15d-PGJ2）, one of the major metabolites from prostaglandin D2 in arachidonic acid metabolic pathway, has potential anti-inflammatory properties. The objective of this study was to explore the effects of 15d-PGJ2-1oaded poly（D,L-lactide-co-glycolide） nanocapsules （15d-PGJ2-NC） on inflammatory responses and bone regeneration in local bone defect. Methods： The study was conducted on 96 Wistar rats from June 2014 to March 2016. Saline, unloaded nanoparticles, free 15d-PGJ2 or 15d-PGJ2-NC, were delivered through a collagen vehicle inside surgically created transcortical defects in rat femurs, lnterleukin-6 （IL-6）, interleukin-1 beta （IL-I~）, and tumor necrosis factor-alpha （TNF-o0 levels in the surrounding soft tissue were analyzed by Western blot and in the defect by quantitative real-time polymerase chain reaction over 14 days. Simultaneously, bone morphogenetic protein-6 （BMP-6） and platelet-derived growth factor-B （PDGF-B） messenger RNA （mRNA） in the defect were examined. New bone formation and EphrinB2 and osteoprotegerin （OPG） protein expression in the cortical defect were observed by Masson＇s Trichrome staining and immunohistochemistry over 28 days. Data were analyzed by one-way analysis of variance. Least-significant difference and Dunnett＇s T3 methods were used with a bilateral P 〈 0.05. Results： Application of 15d-PGJfNC （100μg/ml） in the local bone defect significantly decreased 1L-6, IL-Iβ, and TNF-α mRNA and protein, compared with saline-treated controls （P 〈 0.05）. 15d-PGJfNC upregulated BMP-6 and PDGF-B mRNA （P 〈 0.05）. New bone formation was observed in the cortical defect in 15d-PGJ2-NC-treated animals from 7th day onward （P 〈 0.001）. Expression of EphrinB2 and OPG presented early on day 3 and persisted through day 28 in 15d-PGJfNC group （P 〈 0.05）. Conclusion： Stable 15d-PGJz-NC complexes were prepared that could attenuate IL-6, IL-1 β, and TNF-α expression, while increasing new bone formation and growth factors related to bone regeneration.

关键词： 15-Deoxy-Am4-prostaglandin J, lnterleukin-I Beta Interleukin-6 nanocapsules Osteogenesis

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Magnetic and Microwave-absorption Properties of Graphite-coated (Fe, Ni) nanocapsules

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Journal of Materials Science & Technology 2011年第7期27卷 607-614页

作者： Zhigao Xie Dianyu Geng Xianguo Liu Song Ma Zhidong Zhang Shenyang National Laboratory for Materials Science Institute of Metal Research and International Centre for Material Physics Chinese Academy of Sciences 72 Wenhua Road Shenyang 110016 China

The structure, magnetic and microwave-absorption properties of graphite-coated （Fe, Ni） alloy nanocapsules, synthesized by the arc-discharge method, have been studied. High-resolution transmission electron microscopy shows that the nanocapsules have a core/shell structure with （Fe, Ni） alloy as the core and graphite as the shell. All （Fe, Ni） alloy nanocapsules/paraffin composites show good microwave-absorption properties. The optimal reflection loss （RL） was found for （Fe70Ni30）/C nanocapsules/paraffin composites, being -47.84 dB at 14.6 GHz for an absorber thickness of 1.99 mm, while the RL values exceeding -10 dB were found in the 12.4– 17.4 GHz range, which almost covers the Ku band （12.4–18 GHz）. For （Fe70Ni30）/C nanocapsules/paraffin composites, RL values can exceed -10 dB in the 11.4–18 GHz range with an absorber thickness of 1.91 mm, which cover the whole Ku band.

关键词： Magnetic nanocapsules Microwave absorption

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A new scale for optimized cryogenic magnetocaloric effect in ErAl_2@Al_2O_3 nanocapsules

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Journal of Materials Science & Technology 2018年第5期34卷 848-854页

作者： F.Wei S.Ma L.Yang Y.Feng J.Z.Wang A.Hua X.G.Zhao D.Y.Geng Z.D.Zhang Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of Sciences

The Er Al2@Al2O3 nanocapsules with Er Al2core and Al2O3 shell were synthesized by modified arc-charge technique.The typical core-shell structure of the nanocapsules was confirmed by high resolution transmission electron microscopy and X-ray photoelectron spectroscopy.Transmission electron microscopy analysis shows the irregular sphere of the nanocapules with an average diameter of 26 nm.Magnetic investigation revealed the Curie temperature of Er Al2@Al2O3 nanocapsules at 20 K and the typical superparamagnetic behavior between blocking temperature and Curie temperature.Based on the blocking temperature and average diameter,the magnetocrystalline anisotropy constant of Er Al2@Al2O3nanocapsules was estimated to illustrate the magnetic contribution to the-SM.The large-SMof 14.25 J/（kg K）was obtained under 50 k Oe at 5 K.A vital parameter β was introduced in the present work to scale the optimized magnetic characteristics and the optimized mechanism was discussed in detail according to classical superparamagnetic theory.The results demonstrate that the optimal-SMwill be obtained when the magnetic parameter β is close to the theoretical coefficient.

关键词： nanocapsules Magnetocaloric effect Superparamagnetic property Cryogenic refrigeration

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Structure and Magnetic Properties of Boron-oxide and Boron-nitride Coated Iron nanocapsules

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Journal of Materials Science & Technology 2010年第11期26卷 1051-1056页

作者： W.S. Zhang J.G. Zheng E. Briick P.Z. Si D.Y. Geng Z.D. Zhang Shenyang National Laboratory for Materials Science Institute of Metal Research Chinese Academy of Sciences China and International Centre for Materials Physics Chinese Academy of Sciences Shenyang 110016 China College of Physics & Electronic Engineering Taizhou University Taizhou 317000 China Electron Probe Instrumentation Center (EPIC) Northwestern University 2225N Campus Drive 1156 MLSF Evanston IL 60208-3108 USA Van der Waals-Zeeman Instituut Universiteit van Amsterdam Valckenierstraat 65 1018 XE Amsterdam The Netherlands

The boron-oxide coated iron nanocapsules have been prepared by arc-discharge in a mixture of diborane and nitrogen,and then the boron-nitride coated iron nanocapsules by a subsequent annealing under a nitrogen atmosphere at 1100 ℃.After the arc-discharge,the boron-oxide coated iron nanocapsules form,which show an amorphous surface layer of B2O3 （and/or B） and a core of γ-Fe,α-Fe,FeB phases.After being annealed,part of the α-Fe phase transforms to the γ-Fe phase,and the FeB phase decomposes while the BN phase forms.The BN shell structure formed in the BN encapsulating iron nanocapsules is incomplete.Magnetic properties of the boron-oxide coated and the boron-nitride coated iron nanocapsules were compared and discussed in terms of the particles sizes,the phase components,and the surface structures.

关键词： Arc discharge nanocapsules Magnetic properties

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Synthesis and Characterization of nanocapsules of α-Fe(NiCoAl) Solid-solution

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Journal of Materials Science & Technology 2005年第3期21卷 297-300页

作者： DianyuGENG J.C.Kim C.J.Cho ChonglinCHEN XianguoZHAO ZidongZHANG ShenyangNationalLaboratoryforMaterialsScience InstituteofMetalResearchandInternationalCentretbrMaterialsPhysicsChineseAcademyofSciencesShenyang110016China KoreaInstituteofMachineryandMaterials 66Sangnam-dongChangwonKynugnam640-010SouthKorea

α-Fe(NiCoAl) solid-solution nanocapsules were prepared with pure powders of Fe, Ni, Co and Al by the plasma arc-discharging using a copper crucible. The shapes of the nanocapsules are in polyhedrons with the core/shell structure. The body centered cubic (BCC) phase is formed in the core. The size of the nanocapsules is in the range of 10-120 nm and the thickness of the shell is 4-11 nm. Saturation magnetization JS=150 Am2/kg and coercivity iHC=24.3 kA/m are achieved for the nanocapsules.

关键词： nanocapsules a-Fe solid solution Plasma arc discharge Magnetic properties

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ATP-responsive hollow nanocapsules for DOX/GOx delivery to enable tumor inhibition with suppressed P-glycoprotein

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Nano Research 2021年第1期14卷 222-231页

作者： Huimin Zhu Guodong Cao Yike Fu Chao Fang Qiang Chu Xiang Li Yulian Wu Gaorong Han State Key Laboratory of Silicon Materials School of Materials Science and EngineeringZhejiang UniversityHangzhou 310027China Department of Surgery Second Affiliated Hospital Zhejiang University School of Medicine Zhejiang UniversityHangzhou 310000China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang UniversityHangzhou 311200China

Multidrug resistance(MDR)restricts chemotherapy efficacy due to P-glycoprotein(P-gp)mediated drug efflux,whereas current approaches to suppressing P-gp expression suffer from intrinsic challenges,such as low transfection,high toxicity and poor specificity.Here,hollow ferric-tannic acid complex nanocapsules(HFe-TA),which can be effectively degraded by the reaction with adenosine triphosphate(ATP),are synthesized for the delivery of glucose oxidase(GOx)and doxorubicin(DOX)for tumor treatment.The findings indicate that the intracellular ATP is significantly decreased due to the combined effect of HFe-TA degradation and GOx-mediated glucose consumption.Along with this ATP down-regulation,P-gp expression of tumor cells is suppressed remarkably,which in turn promotes the intracellular accumulation and anticancer efficacy of DOX.In addition,the production of•OH by Fe ions released from HFe-TA is promoted by the by-products of the oxidation of glucose process by GOx.In consequence,HFe-TA nanocapsules loaded with DOX and GOx enable significant inhibition effect to tumors both in vitro and in vivo due to the synergistic effect of cascade reactions.This study has therefore provided an alternative therapeutic platform for effective tumor inhibition with the potential in overcoming intrinsic MDR.

关键词： adenosine triphosphate(ATP)-responsive P-glycoprotein(P-gp)suppression nanocapsules multi-model tumor therapy

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Enhanced antibacterial activity of cotton via silver nanocapsules deposited by atmospheric pressure plasma jet

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Plasma Science and Technology 2023年第3期25卷 130-137页

作者：张潇漫马晓萍李茂洋季佩宇黄天源诸葛兰剑吴雪梅 School of Physical Science and Technology Soochow UniversitySuzhou 215006People’s Republic of China Collaborative Innovation Center of Suzhou Nano Science and Technology Soochow UniversitySuzhou 215006People’s Republic of China The Key Laboratory of Thin Films of Jiangsu Soochow UniversitySuzhou 215006People’s Republic of China Analysis and Testing Center Soochow UniversitySuzhou 215123People’s Republic of China

In this work,the antibacterial activity of cotton containing silver nanocapsules prepared by atmospheric pressure plasma(APP)deposition is investigated.The nanocapsules consist of a shell and a silver nanoparticle(Ag NP)core,where the core is used to bring antibacterial activity,and the shell is utilized to suppress the potential toxicity of Ag NPs.The surface morphology and the elements of the samples are analyzed by scanning electron microscopy(SEM),energy dispersive x-ray and x-ray photoelectron spectroscopy(XPS).The SEM results show that the skin of the cotton fibers will fall off gradually after APP treatment over 3 min,and the XPS results show that the Ag content will rise to 1.6%after APP deposition for 10 min.Furthermore,the antimicrobial activity tests show that the reduction rates of Escherichia coli and Staphylococcus aureus can achieve 100%when the sample is treated for 10 min,which exhibits excellent antibacterial activity.In addition,the UV absorption properties of the cotton will also be correspondingly improved,which brings a broader application prospect for antibacterial cotton.

关键词： atmospheric pressure plasma(APP) nanocapsules antimicrobial activity cotton

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