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中国药学杂志 2012年第23期47卷 1883-1885页

作者：刘新利李瑶瑶许晓坤鲁春华山东轻工业学院教育部生物化学重点实验室山东省微生物工程重点实验室济南250353 山东大学药学院教育部生物化学重点实验室济南250012

目的对黏球菌(Myxococcus sp.)189B05大罐发酵吸附树脂提取物的化学成分进行研究。方法采用Sephadex LH-20和硅胶等色谱柱色谱进行分离纯化,用波谱技术鉴定化合物的结构。结果从70 L发酵提取物中分离得到5个化合物,分别为quinolin-4-ylm... 详细信息

目的对黏球菌(Myxococcus sp.)189B05大罐发酵吸附树脂提取物的化学成分进行研究。方法采用Sephadex LH-20和硅胶等色谱柱色谱进行分离纯化,用波谱技术鉴定化合物的结构。结果从70 L发酵提取物中分离得到5个化合物,分别为quinolin-4-ylmethanol(1),myxothiazol(2),4-quinolinecarboxaldehyde oxime(3),N-(2-phenylethyl)acetamide(4),N-acetyltyra-mine(5)。结论化合物1～5均为首次从该菌株中分离得到。

关键词：黏球菌189B05 柱色谱结构鉴定 myxothiazol

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Impact of inhibition of Qo and Qi site of mitochondrial complex Ⅲ on persistent sodium currents and neuron excitability in rat hippocampal CA1 cells

Impact of inhibition of Qo and Qi site of mitochondrial comp...

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中国神经科学学会第六届学术会议暨学会成立十周年庆祝大会

作者： Lai Bin, Zhang Li, Dong Yan-lian, Zhu Yan-hua, Sun Feng-yan, Zheng Ping State Key Laboratory of Medical Neurobiology, Fudan University Shanghai Medical College, Shanghai 200032,China

＜正＞Hypoxia-induced inhibition of Qo and Qi site of mitochondrial complex Ⅲunder hypoxia has received attention, but its downstream pathways remain unclear. In this paper, we used the Qo site inhibitor myxothiazol and the Qi site,inhibitor antimycin A to mimick the inhibition of the Qo and Qi site of mitochondrial complex Ⅲand studied the effects of the inhibition of these site on persistent sodium currents, transient sodium currents and neuron excitability in rat hippocampal CA1 cells with whole cell patch-clamp methods combined biochemical and pharmacological approaches. The results showed that:

关键词： hippocampus mitochondrial myxothiazol antimycin A persistent sodium currents transient sodium currents neuron excitability whole-cell patch-clamp protein kinase C reactive oxygen species

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Inhibition of Qi and Qo sites of mitochondrial complexⅢinduced opposite effects on HVA calcium channels via activation of different protein kinase C isoforms in cortical neurons

Inhibition of Qi and Qo sites of mitochondrial complexⅢindu...

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中国神经科学学会第四次会员代表大会暨第八届全国学术会议

作者： Pei-Ying WU,Bin LAI,Ping ZHENG~* State Key Laboratory of Medical Neurobiology,Fudan University,Shanghai Medical College,Shanghai 200032, China

＜正＞Objective Inhibition of Qi and Qo sites of mitochondrial complexⅢunder hypoxia and ischemia has received attention,but its downstream targets remain largely *** work showed altered function of sodium channels after the inhibition of complexⅢ.HVA calcium channels were well recognized to be an important factor in ischemia-induced cell *** explore whether the inhibition of mitochondrial complexⅢhas any effect on the function of HVA calcium channels and the related downstream pathways will shed light on our understanding about the complicated events after hypoxia/*** Electrophysiological recording of HVA calcium currents was carried out on slice preparations from 14-day-old rats using whole cell recording *** of Qi and Qo sites of mitochondrial complexⅢwas mimicked by application of specific inhibitors antimycin A and myxothiazol *** drugs were applied through the bath ***（1） Antimycin A decreased the peak amplitude of HVA calcium current while myxothiazol increased it.（2） Antimycin A induced the effect via the activation ofβ1 isoform of protein kinase C while myxothiazol increased the amplitude of calcium currents through the activation ofδisoform.（3） The effect of antimycin A on HVA calcium channels could be abolished by the application of antioxidants PHEN and MPG while the superoxide scavenger MnTB Aβand SOD could cancel the effect of *** Inhibition of mitochondrial complex HI at different sites exerted opposite effects on HVA calcium channels.

关键词： cortical neuron whole cell patch-clamp mitochondria complexⅢ Qi site Qo site antimycin A myxothiazol HVA calcium channels Protein Kinase C isoforms reactive oxygen species superoxide

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