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Journal of Integrative Agriculture 2017年第11期16卷 2646-2654页

作者： XIN Jian-zeng LI Zheng LI Xin LI Meng WANG Ying YANG Fu-min ZHANG De-quan College of Food Science and Engineering Gansu Agricultural University Institute of Food Science and Technology Chinese Academy of Agricultural Sciences

This study investigated the influence of activities of glyceraldehyde-3-phosphate dehydrogenase （GAPDH） and lactate dehydrogenase-B （LDH-B） on the colour stability of mutton. From 60 sheep （Bayannur mutton sheep）, 15 Iongissimus dorsi （LD） muscles were selected on the basis of colour stability （R630/580 and a＊ value） during the storage and classified into three groups （5 for each group） as high colour stability （HCS）, intermediate colour stability （ICS） and low colour stability （LCS）. The activities of GAPDH and LDH-B, muscle colour attributes, nicotinamide adenine dinucleuotide （NADH） concen- tration and lactate concentration were measured. The samples in HCS had higher activities of GAPDH and LDH-B than the samples in the LCS, and the samples in the HCS group also possessed higher NADH and lower lactate concentration. The higher activity of dehydrogenase enzyme may result in higher NADH concentrations and colour stability in muscle tissue. The results suggest that the activity of GAPDH and LDH-B may also play a role in maintaining colour stability.

关键词： mutton meat colour GAPDH LDH-B NADH

Microbiological Quality of Fresh and Grilled mutton Sold in Ouagadougou, Burkina Faso

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Food and Nutrition Sciences 2022年第12期13卷 986-1000页

作者： Zoénabo Douamba Abel Tankoano Donatien Kaboré Diarra Compaore-Sereme Mahamoudou Ouédraogo Pingdwindé Marie Judith Samadoulougou-Kafando Adama Paré Mamadou Hama Dicko Hagrétou Sawadogo/Lingani Institut de Recherche en Sciences Appliquées et Technologies (IRSAT)/Centre National de la Recherche Scientifique et Technologique (CNRST) Ouagadougou Burkina Faso Institut de Recherche en Sciences Appliquées et Technologies (IRSAT)/Centre National de la Recherche Scientifique et Technologique (CNRST) Bobo-Dioulasso Burkina Faso Laboratoire de Biochimie Biotechnologie Technologie Alimentaire et Nutrition (LABIOTAN) UFR/SVT Université Joseph KI-ZERBO Ouagadougou Burkina Faso

In Ouagadougou, grilled meats in the form of pieces and brochettes are very popular and well-known to consumers. The aim of this study was to assess the microbial quality of mutton meat sold in Ouagadougou, Burkina Faso. A total of 120 samples were collected from 20 meat grillers 60 samples of fresh meat and 60 samples of grilled meat. The sampling was done between the month of August 2018 and the month of February 2019. The samples were analyzed according to standard methods. The test performed were counts of Aerobic Mesophilic Bacteria (AMB), yeasts and molds, enterobacteria, Campylobacter spp, Staphylococcus aureus, Bacillus cereus, Brucella spp, Pseudomonas aeruginosa, the search for salmonella, and the detection of antibiotics residues. Results showed a high count of AMB (8.77 and 6.78 log UFC/g);enterobacteria (6.58 and 3.05 log UFC/g), Staphylococcus aureus (6.45 and 4.35 log UFC/g), Bacillus cereus (6.98 and 4.52 log UFC/g), Campylobacter (6.03 and 3.86 log UFC/g), yeasts and molds (4.80 and 3.26 log UFC/g) and Pseudomonas aeruginosa (0.45 and 0.15 log UFC/g), respectively in fresh meat and grilled meat. Presumptive Salmonella was found in 95% of fresh meat samples and in 75% of grilled meat samples. In the tested samples, no Brucella spp were detected. However, residues of antibiotics were found in 5% of fresh meat samples and 5% of grilled meat samples. Means of moisture and pH were respectively 74.91% and 6.05% for fresh meat and 53.21% and 6.06% for grilled meat. The average microbial counts recorded in fresh and grilled meat are significantly high and indicate poor hygiene in the raw material and ready-to-eat meat. Good practices of hygiene and processing guides should be developed for the meat grilling value chain actors to reduce contamination risks.

关键词： Meat mutton Grilling Microbial Quality

Determination of 14 Organophosphorus Pesticide Residues in mutton by Gel Permeation Chromatography-Gas Chromatography-Mass Spectrometry(GPC-GC-MS)

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Agricultural Biotechnology 2024年第3期13卷 28-30,33页

作者： Junpeng ZHAO Richard Avoi Azman Bin Atil@Azmi Jiao CHEN Ling YUN Tangshan Food and Drug Comprehensive Inspection and Testing Center Tangshan 063000China Hebei Agricultural Products Quality and Safety Testing Technology Innovation Center Tangshan 063000China Tangshan Institute of Industrial Technology for Functional Agricultural Products Tangshan 063000China Universiti Malaysia Sabah Faculty of Medicine and Health SciencesMalaysia Guizhou Forerunner College Guiyang 550000China Tangshan Center for Disease Prevention and Control Tangshan 063000China

[Objectives]This study was conducted to purify mutton samples by gel permeation chromatography(GPC).[Methods]Fourteen organophosphorus pesticide residues in samples were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS)in selective ion scanning mode(SIM).[Results]The organophosphorus pesticide standard solutions showed good linearity in the mass concentration range of 0.1-10.0μg/ml with correlation coefficients(r)not lower than 0.999,and the detection limits(S=3 N)ranged from 0.01 to 0.05 mg/*** average recovery values were in the range of 80.2%-99.7%,with relative standard deviations(RSDs,n=3)in the range of 1.8%-6.3%,at the addition levels of 0.5,1.0 and 2.0 mg/kg.[Conclusions]The method is simple,sensitive and accurate,and can be used for the determination of organophosphorus pesticide residues in mutton.

关键词： mutton Gas chromatography-mass spectrometry Gel permeation chromatography Organophosphorus Pesticide residue

Simultaneous Determination of 14 β-Receptor Agonists Residues in mutton by High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS/MS)

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Agricultural Biotechnology 2023年第5期12卷 55-58页

作者： Zhe MENG Jianhua WANG Bo LIU Yuhang GUO Haoshuang DONG Pingyang SHAN Dawei WANG Yajuan SONG Tangshan Food and Drug Comprehensive Testing Center Tangshan 063000China

[Objectives]A high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for the determination of 14β-receptor agonist residues in mutton.[Methods]Samples were hydrolyzed byβ-glucuronidase and extracted with 5%acetic acid-acetonitrile(1:99,V/V)*** Eclipse plus C 18 column was used for separation,and the MRM mode was used for qualitative analysis,and the external standard method was used for quantitative analysis of matrix standard solutions.[Results]Under the optimal conditions,the retention time of the 14 kinds ofβ-receptor agonists ranged from 1.0 to 9.5 *** the mass concentration was in the range of 0.05-0.50μg/ml,the linear relationship ofβ-receptor agonists was good,with correlation coefficients(r)≥*** detection limits of the method were in the range of 0.04-0.87μg/kg,and the quantitative limits were in the range of 0.35-1.86μg/*** average recovery values were in the range of 82.8%-108.9%,with RSDs(n=6)in the range of 1.9%-6.7%.[Conclusions]The method is simple,sensitive,reproducible,accurate,and can be used for simultaneous determination of the 14 kinds ofβ-receptor agonist residues in mutton.

关键词： mutton High performance liquid chromatography-tandem mass spectrometry β-receptor agonist Residue

Determination of Selected Heavy Metals in the Organs of Some Animals Reared in the Gold-Mining Areas of Zamfara State, Nigeria

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Journal of Agricultural Chemistry and Environment 2018年第4期7卷 188-202页

作者： U. A. Birnin-Yauri M. K. Musa S. M. Alhaji Department of Pure and Applied Chemistry Usmanu Danfodiyo University Sokoto Nigeria Department of Chemistry Sokoto State University Sokoto Nigeria

The study was conducted to determine the concentrations of heavy metals (Cu, Zn, Co, Mn, Fe, Cr, Cd and Pb) in the liver, kidney and flesh of Cattle, Sheep and Goats from Anka and Bukkuyum Local Government areas of Zamfara State Nigeria. The levels of heavy metals in the liver, kidney and flesh of beef, mutton and caprine ranged from 56 to 549 μg·g-1 for Zn;109 to 576 μg·g-1 for Fe;0.0 to 4.0 μg·g-1 for Pb;2.52 to 13.25 μg·g-1 for Mn;0.00 to 19.64 μg·g-1 for Cr;0.00 to 0.25 μg·g-1 for Cd;0.00 to 2.71 μg·g-1 for Co and 3.59 to 538 μg·g-1 for Cu. The concentration of Cr, Pb and Mn in the kidneys and livers of the samples were observed to be higher than the maximum allowable level. Similarly, the exception of Cr in the liver of sheep and goat from Anka and liver of sheep from Bukkuyum, Pb in some liver and kidney samples and Mn in some liver, kidney samples and flesh of cattle from Anka were found to be polluted and hence unsafe for consumption.

关键词： Heavy Metals Caprine mutton Beef Environment Cattle Sheep Goats