检索结果-南通市图书馆

The regulating role of mutant ikbα in expression of TIMP-2 and MMP-9 in human glioblastoma multiform

Chinese Medical Journal 2009年第2期122卷 205-211页

作者： HU Yu-hua YU Li-Jie SHAO En-de WU Jian-liang JI Jian-wen Department of Neurosurgery Second Hospital of Hebei Medical University Shijiazhuang Hebei 050000 China Department of Pathology Stomatological Hospital of Hebei Medical University Shijiazhuang Hebei 050000 China Department of Neurosurgery Langfang People's Hospital Langfang Hebei 065000 China

Background Our previous studies demonstrated that mutant ikbα （ikbαM） inhibited the occurrence, growth and angiogenesis of human glioblastoma multiform （GBM）. However, the specific mechanism by which ikbaM regulates protein-degrading enzymes secreted from GBM to inhibit invasion and metastasis has remained unclear. The aim of the present study was to investigate the regulatory role and significance of ikbαM genes in the expression of tissue inhibitor of metalloproteinase （TIMP）-2 and matrix metalloproteinase （MMP）-9 in human GBM. Methods We established the following four GBM cell lines stably expressing ikbaM by plasmid construction, gene transfection and screening for ikbαM protein expression： mutant ikba-transfected cells （G36A-M）, wild-type ikba-transfected cells （G36A-W）, empty plasmid transfected cells （G36A-P） and untransfected cells （G36A）. The TIMP-2 and MMP-9 expression was detected by RT-PCR and Western blotting. Tumor cells were then implanted subcutaneously into nude mice to establish an animal model of ectopic tumor growth, and TIMP-2 and MMP-9 expression was determined by immunohistochemical methods. Results The results showed that there was a significant increase in TIMP-2 expression and a significant decrease in MMP-9 expression in the G36A-M group at both the RNA and protein levels compared with the G36A-W group, G36A-P group and G36A group. Similar results were observed in the immunohistochemical staining analysis of tumor tissues. In the G36A-M group, TIMP-2 expression was significantly higher while MMP-9 expression was significantly lower than in the other three groups. Conclusions Our findings indicate that ikbaM inhibits the activation of NF-KB. It significantly up-regulates TIMP-2 expression in human malignant glioma cells and down-regulates the expression of MMP-9. Thus, ikbαM maintains the integrity of the extracellular matrix and further inhibits the growth and metastasis of tumor tissues. Chin Med J 2009; 122（2）：205-211

关键词： nuclear factor-KB mutant-type ikbα metalloproteinases extracellular matrix glioma

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：