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Skeletal muscle-derived cells repair peripheral nerve defects in mice

Neural Regeneration Research 2020年第1期15卷 152-161页

作者： Zi-Xiang Chen Hai-Bin Lu Xiao-Lei Jin Wei-Feng Feng Xiao-Nan Yang Zuo-Liang Qi The 16^th Department Plastic Surgery HospitalChinese Academy of Medical Sciences(CAMS)and Peking Union Medical College(PUMC)BeijingChina Yu Tian Cheng Plastic Surgery Clinic ShanghaiChina

Skeletal muscle-derived cells have strong secretory function,while skeletal muscle-derived stem cells,which are included in muscle-derived cells,can differentiate into Schwann cell-like cells and other cell ***,the effect of muscle-derived cells on peripheral nerve defects has not been *** this study,5-mm-long nerve defects were created in the right sciatic nerves of mice to construct a peripheral nerve defect *** female C57BL/6 mice were randomly divided into four *** the muscle-derived cell group,muscle-derived cells were injected into the catheter after the cut nerve ends were bridged with a polyurethane *** external oblique muscle-fabricated nerve conduit and polyurethane groups,an external oblique muscle-fabricated nerve conduit or polyurethane catheter was used to bridge the cut nerve ends,*** the sham group,the sciatic nerves on the right side were separated but not *** 8 and 12 weeks post-surgery,distributions of axons and myelin sheaths were observed,and the nerve diameter was calculated using immunofluorescence *** number,diameter,and thickness of myelinated nerve fibers were detected by toluidine blue staining and transmission electron *** fiber area ratios were calculated by Masson’s trichrome staining of gastrocnemius muscle *** functional index was recorded using walking footprint analysis at 4,8,and 12 weeks after *** results showed that,at 8 and 12 weeks after surgery,myelin sheaths and axons of regenerating nerves were evenly distributed in the muscle-derived cell *** number,diameter,and myelin sheath thickness of myelinated nerve fibers,as well as gastrocnemius muscle wet weight and muscle area ratio,were significantly higher in the muscle-derived cell group compared with the polyurethane *** 4,8,and 12 weeks post-surgery,sciatic functional index was notably increased in the muscle-derived cell group compared with the polyurethane

关键词： muscle nerve conduit myokine nerve regeneration nerve repair peripheral nerve regeneration polyurethane catheter seed cells skeletal muscle skeletal muscle-derived cells tissue-engineered nerve

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Research progress in muscle-derived stem cells Literature retrieval results based on international database

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Neural Regeneration Research 2012年第10期7卷 784-791页

作者： Li Zhang, Wei Wang Jinzhou Clinical College of Liaoning Medical University Jinzhou Central Hospital, Jinzhou 121000, Liaoning Province, China Jinzhou Clinical College of Liaoning Medical University Jinzhou Central Hospital Jinzhou 121000 Liaoning Province

OBJECTIVE： To identify global research trends of muscle-derived stem cells （MDSCs） using a bibliometric analysis of the Web of Science, Research Portfolio Online Reporting Tools of the National Institutes of Health （NIH）, and the Clinical Trials registry database （***）. DATA RETRIEVAL： We performed a bibliometric analysis of data retrievals for MDSCs from 2002 to 2011 using the Web of Science, NIH, and ***. SELECTION CRITERIA： Inclusion criteria：（1） Web of Science：（a） peer-reviewed articles on MDSCs that were published and indexed in the Web of Science. （b） Type of articles： original research articles, reviews, meeting abstracts, proceedings papers, book chapters, editorial material and news items. （c） Year of publication： 2002-2011. （d） Citation databases： Science Citation Index-Expanded （SCI-E）, 1899-present; Conference Proceedings Citation Index-Science （CPCI-S）, 1991-present; Book Citation Index-Science （BKCI-S）, 2005-present. （2） NIH：（a） Projects on MDSCs supported by the NIH. （b） Fiscal year： 1988-present. （3） ***： All clinical trials relating to MDSCs were searched in this database. Exclusion criteria：（1） Web of Science：（a） Articles that required manual searching or telephone access. （b） We excluded documents that were not published in the public domain. （c） We excluded a number of corrected papers from the total number of articles. （d） We excluded articles from the following databases： Social Sciences Citation Index （SSCI）, 1898-present; Arts ＆ Humanities Citation Index （A＆HCI）, 1975-present; Conference Proceedings Citation Index - Social Science ＆ Humanities （CPCI-SSH）, 1991-present; Book Citation Index - Social Sciences ＆ Humanities （BKCI-SSH）, 2005-present; Current Chemical Reactions （CCR-EXPANDED）, 1985-present; Index Chemicus （IC）, 1993-present. （2） NIH：（a） We excluded publications related to MDSCs that were supported by the NIH. （b） We limited the keyword search to studies that included MDSCs within th

关键词： stem cells muscle-derived regeneration Web of Science NIH Clinical Trials bibliometric

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World Journal of Stem Cells 2019年第6期11卷 347-374页

作者： Urszula Kozlowska Agnieszka Krawczenko Katarzyna Futoma Tomasz Jurek Marta Rorat Dariusz Patrzalek Aleksandra Klimczak Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of SciencesWroclaw 53-114Poland Department of Forensic Medicine Wroclaw Medical UniversityWroclaw 50-345Poland Faculty of Health Science Department of PhysiotherapyWroclaw Medical UniversityWroclaw 50-367Poland

BACKGROUND Mesenchymal stromal/stem cells (MSCs) constitute a promising tool in regenerative medicine and can be isolated from different human tissues. However, their biological properties are still not fully characterized. Whereas MSCs from different tissue exhibit many common characteristics, their biological activity and some markers are different and depend on their tissue of origin. Understanding the factors that underlie MSC biology should constitute important points for consideration for researchers interested in clinical MSC application. AIM To characterize the biological activity of MSCs during longterm culture isolated from: bone marrow (BM-MSCs), adipose tissue (AT-MSCs), skeletal muscles (SMMSCs), and skin (SK-MSCs). METHODS MSCs were isolated from the tissues, cultured for 10 passages, and assessed for: phenotype with immunofluorescence and flow cytometry, multipotency with differentiation capacity for osteo-, chondro-, and adipogenesis, stemness markers with qPCR for mRNA for Sox2 and Oct4, and genetic stability for p53 and c-Myc;27 bioactive factors were screened using the multiplex ELISA array, and spontaneous fusion involving a co-culture of SM-MSCs with BM-MSCs or AT-MSCs stained with PKH26 (red) or PKH67 (green) was performed. RESULTS All MSCs showed the basic MSC phenotype;however, their expression decreased during the follow-up period, as confirmed by fluorescence intensity. The examined MSCs express CD146 marker associated with proangiogenic properties;however their expression decreased in AT-MSCs and SM-MSCs, but was maintained in BM-MSCs. In contrast, in SK-MSCs CD146 expression increased in late passages. All MSCs, except BM-MSCs, expressed PW1, a marker associated with differentiation capacity and apoptosis. BM-MSCs and AT-MSCs expressed stemness markers Sox2 and Oct4 in long-term culture. All MSCs showed a stable p53 and c-Myc expression. BM-MSCs and AT-MSCs maintained their differentiation capacity during the follow-up period. In contrast

关键词： Mesenchymal stem/progenitor cells Bone marrow MSCs Adipose tissue MSCs muscle-derived MSCs Skin-derived MSCs Cytokines and trophic factors of MSCs Spontaneous fusion of MSCs

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