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Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs

Forensic Sciences Research 2020年第4期5卷 292-299页

作者： Feng Liu Fei Jia Fang Sun Bin Zhao Hongying Shen Liaoning Provincial Public Security Department ShenyangChina

This article describes a newly devised autosomal short tandem repeat(STR)multiplex polymer-ase chain reaction(PCR)system for 19 autosomal loci(D12S391,D13S317,D16S539,D18S51,D19S433,D2S1338,D21S11,D3S1358,D5S818,D6S1043,D7S820,D8S1179,CSF1PO,FGA,TH01,TPOX,vWA,Penta D and Penta E),27 Y-chromosome STR loci(DYS19,DYS385,DYS3891,DYS38911,DYS390,DYS391,DYS392,DYS393,DYS437,DYS438,DYS439,DYS448,DYS449,DYS456,DYS458,DYS460,DYS481,DYS518,DYS533,DYS570,DYS576,DYS635,DYS627,YGATAH4 and DYF387S1)and amelogenin with six-colour fluorescent *** parameters were evaluated,such as its accuracy,sensitivity,specificity,stability,ability to ana-lysis of mixtures and effects of changes in the PCR-based *** of the 47 selected STR loci were accurately and robustly amplified from 282 bloodstain *** species-spe-cificity was high and some ability to inhibit Hematin was *** lowest detectable DNA amount was ≥0.125 *** of the male loci of the secondary component were revealed precisely when the control DNA was mixed at male/female and male/male ratios of 1:4 or *** conclude that the present 19-plex autosomal STR and 27 Y-STR assay is both accur-ate and *** constitutes an additional powerful tool for forensic applications.

关键词： Forensic sciences forensic genetics autosomal STR Y-STR multiplex amplification validation

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一种新型6色荧光25个STR基因座复合扩增体系研究

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中国法医学杂志 2020年第3期35卷 258-264页

作者：白雪姚伊人叶健 Institute of Forensic Science Ministry of Public SecurityP.R.ChinaBeijing 100038

To develop and validate a novel 6-dye STR(short tandem repeat)25-plex DNA typing system for forensic DNA profiling and *** this study,a novel STR 25-plex DNA typing system that includes 24 autosomal STRs(D1S1656,D2S1338,D2S441,D3S1358,D5S818,D6S1043,D7S820,D8S1179,D10S1248,D12S391,D13S317,D16S539,D18S51,D19S433,D21S11,D22S1045,CSF1PO,FGA,Penta D,Penta E,TH01,TPOX,vWA,D11S4463)and Amelogenin was *** studies demonstrated the sensitivity,accuracy,and reproducibility of our novel STR 25-plex DNA typing *** sensitivity of the STR 25-plex DNA typing system was demonstrated by the ability to obtain complete profiles from as little as 0.125ng of human *** testing was demonstrated by the lack of cross-reactivity to a variety of commonly encountered animal species and microbial *** stability testing,full profiles were obtained with humic acid concentration≤60ng/μL and hematin≤600μ*** forensic evaluation,the selected 24 autosomal STRs followed the Hardy-Weinberg *** 24 autosomal STRs were independent from one another,PM(Probability matching)was 3.5434×10-28,TDP(Total Probability of Discrimination Power)was 0.999999999999999999999999969863,and CEP(Cumulative probability of exclusion)was *** new STR 25-plex typing system is sensitive,reproducible,and stable,therefore it is highly applicable for use in national DNA database and can help to facilitate international data sharing.

关键词： Forensic genetics 6-dye STR multiplex amplification

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