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multilocus sequence typing indicates diverse origins of invasive Candida tropicalis isolates in China

Chinese Medical Journal 2014年第24期127卷 4226-4234页

作者： Fan Xin Xiao Meng Wang He Zhang Li Kong Fanrong Lu Juan Hu Zhidong Kang Mei Xu Yingchun Graduate School Peking Union Medical College Chinese Academy of Medical Sciences Beijing 100730 China Department of Clinical Laboratory Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China Centre for Infectious Diseases and Microbiology Laboratory Services Westmead Hospital Darey Road Westmead New South Wales Australia Department of Clinical Laboratory First Affiliated Hospital of Harbin Medical University Harbin Heilongiiang 150001 China Department of Clinical Laboratory Tianjin Medical University General Hospital Tianjin 300052 China Laboratory of Clinical Microbiology West China Hospital Sichuan University Chengdu Sichuan 610041 China

Background According to data from the China Hospital Invasive Fungal Surveillance Net （CHIF-NET） 2010, Candida tropica/is （C. tropica/is） is the third most common pathogen causing invasive candidiasis. Moreover, the majority of fluconazole-resistant C. tropicalis isolates were from a single hospital. Therefore, a molecular epidemiological survey is necessary to investigate the genetic relatedness of C. tropica/is isolates in China. Methods In this study, 48 C. tropicalis isolates causing invasive fungal infections from four tertiary hospitals in China were studied. All the isolates were identified by sequencing the internal transcribed spacer region. Antifungal susceptibility to triazoles, amphotericin B, and caspofungin was determined by the Clinical and Laboratory Standards Institute standard broth microdilution method. multilocus sequence typing （MLST） was performed, and phylogenetic analysis was further performed by the eBURST and maximum parsimony （MP） methods to characterize the genetic relatedness of isolates. Results MLST discriminated 40 diploid sequence types （DSTs） among 48 isolates, including 36 novel DSTs, and the XYR1 gene showed the highest discriminatory power. The DSTs obtained from this study were compared with those of previously reported C. tropicalis isolates, and there was poor type alignment with regional strains. Nine groups and 11 singletons were identified by eBURST, whereas two groups and 10 subgroups were clustered by MP analysis. Generally, there were no obvious correlations between clonal clusters generated and the specimen source or hospital origin. Seven fiuconazole-resistant isolates were confirmed and assigned to three distinguishable branches. Conclusions The results suggested diverse origins of invasive C. tropicalis isolates in China. Although most invasive C. tropicalis strains in the mainland of China were clustered with previously characterized Asian isolates, major C. tropicalis clusters identified in this study were genetically distinct from those of other geographic regi

关键词： Candida tropicalis invasive fungal infections multilocus sequence typing phylogenetic analysis

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Optimization of Bartonella henselae multilocus sequence typing scheme using single-nucleotide polymorphism analysis of SOLID sequence data

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Chinese Medical Journal 2012年第13期125卷 2284-2288页

作者： ZHAO Fan Gemma Chaloner Alistair Darby SONG Xiu-ping LI Dong-mei Richard Birtles LIU Qi-yong State Key Laboratory for Infectious Diseases Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China Department of Epidemiology Beijing Institute of Heart Lung and Blood Vessel Diseases Beijing An Zhen Hospital Capital Medical University Beijing 100029 China National Centre for Zoonosis Research School of Veterinary Science University of Liverpool Cheshire CH64 7TE UK Centre for Genomics Research Biosciences Building University of Liverpool Crown Street Liverpool L69 7ZB UK School of Environment and Life Sciences University of Salford M5 4WT UK

Background Multi-locus sequence typing （MLST） is widely used to explore the population structure of numerous bacterial pathogens. However, for genotypically-restricted pathogens, the sensitivity of MLST is limited by a paucity of variation within selected loci. For Bartonella henselae （B. henselae）, although the MLST scheme currently used has been proven useful in defining the overall population structure of the species, its reliability for the accurate delineation of closely-related sequence types, between which allelic variation is usually limited to, at most, one or two nucleotide polymorphisms. Exploitation of high-throughput sequencing data allows a more informed selection of MLST loci and thus, potentially, a means of enhancing the sensitivity of the schemes they comprise. Methods We carried out SOLID resequencing on 12 representative B. henselae isolates and explored these data using single nucleotide polymorphism （SNP） analysis. We determined the number and distribution of SNPs in the genes targeted by the established MLST scheme and modified the position of loci within these genes to capture as much genetic variation as possible. Results Using genome-wide SNP data, we found the distribution of SNPs within each open reading frame （ORF） of MLST loci, which were not represented by the established B. henselae MLST scheme. We then modified the position of loci in the MLST scheme to better reflect the polymorphism in the ORF as a whole. The use of amended loci in this scheme allowed previously indistinguishable ST1 strains to be differentiated. However, the diversity of B. henselae was still rare in China. Conclusions Our study demonstrates the use of SNP analysis to facilitate the selection of MLST loci to augment the currently-described scheme for B. henselae. And the diversity among B. henselae strains in China is markedly less than that observed in B. henselae populations elsewhere in the world.

关键词： Bartonella henselae multilocus sequence typing single-nucleotide polymorphism

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Serological and molecular capsular typing, antibiotic susceptibility and multilocus sequence typing of Streptococcuspneumoniae isolates from invasive and non-invasive infections

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Chinese Medical Journal 2013年第12期126卷 2296-2303页

作者： ZHANG Yi-jie CHEN Yu-shen WANG Zhan-wei LI Yu-qian WANG Da-xuan SHANG Ying FU Rong-rong HU Ying-hui GENG Rong WEI Li-ping YANG Jing-ping LI Jia-shu YU Qin DU Juan GAO Zhan-cheng Department of Respiratory and Critical Care Medicine (Zhang YJ Li YQ Shang Y Fu RR and Gao ZC) Department of Clinical Laboratory Peking University People's Hospital Beijing 100044 China Department of Respiratory Medicine Fujian Provincial Hospital Fujian Medical University Fuzhou Fujian 350001 China Department of Respiratory Medicine Beijing Children's Hospital Affiliated to Capital Medical University Beijing 100045 China Department of Emergency MedicineBeijing Children's Hospital Affiliated to Capital Medical University Beijing 100045 China Department of Respiratory Medicine Third Affiliated Hospital of Guangzhou Medical College Guangzhou Guangdong 510150 China Department of Respiratory and Critical Care Medicine Third Affiliated Hospital of the Inner Mongolia Medical College Baotou Inner Mongolia 014030 China Department of Respiratory Medicine Lianyungang First People's Hospital Affiliated to Xuzhou Medical College Lianyungang Jiangsu 222002 China Department of Respiratory Medicine First Hospital of Lan Zhou University Lanzhou Gansu 730000 China Department of Respiratory Medicine Affiliated Hospital of Guiyang Medical College Guiyang Guizhou 550004 China

Background Streptococcus pneumoniae （S.pneumoniae） is a major causative agent of severe infections,including sepsis,pneumonia,meningitis,and otitis media,and has become a major public health concern.We report the pneumococcal serotype and sequence type （ST） distribution,and antimicrobial resistance of 39 S.pneumoniae strains from seven hospitals in China.Methods Blood/cerebrospinal fluid （CSF） and sputum isolates from patients were analyzed to determine S.pneumoniae serotypes by polymerase chain reaction （PCR） and the Neufeld Quellung reaction,the multilocus sequence types （MLST） by PCR and sequencing,and susceptibility to antimicrobial agents by the VITEK Gram Positive Susceptibility Card.Results A total of 39 isolates were collected including 21 blood/CSF and 18 sputum isolates.Conventional serotyping by the Quellung reaction required 749 reactions.In contrast,PCR based typing needed only 106 PCR reactions.The most frequent serotypes from the blood/CSF isolates were 14 （38.1%）,19A （14.3%）,23F （9.5%）,and 18C （9.5%）.In the sputum isolates the most frequent serotypes were 19F （33.3%）,23F （16.7%）,19A （11.1%）,and 3 （11.1%）.The incidence of penicillin resistance in the blood/CSF and sputum isolates was 66.7% and 55.6%,respectively.Statistical analysis showed that patients ≤5 years old had a higher resistance to penicillin when they compared with the patients ≥65 years old （P=0.011）.Serotypes 14,19A and 19F were significantly associated with penicillin resistance （P 〈0.001）.ST320,ST271,and ST876 isolates showed high resistant rates to several antibiotics including penicillin （P=0.006）.All of the isolates of serotype 19A were resistant to both penicillin and erythromycin,and they were all multi-drug resistant （MDR） isolates.Conclusions The specificity and sensitivity of multiplexPCR are good,and this method represents a substantial savings of time and money,and can be widely used in the laboratory and clinical practice.Data from this research showed an extremely high prevalence of penicillin resistance and an increasin

关键词： serotype multilocus sequence typing antibiotic resistance Streptococcus pneumonia

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multilocus sequence typing and population genetic characterization of Cyclospora cayetanensis

Multilocus sequence typing and population genetic characteri...

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中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工...

作者： Yaqiong Guo Yaoyu Feng Na Li Kevin Tang Michael Frace Michael J. Arrowood Dawn M.Roellig Lin Wang Longxian Zhang Lihua Xiao East China University of Science and Technology

Objectives: Cyclospora cayetanensis is an important foodborne pathogen, causing numerous cyclosporiasis outbreaks in North America since the mid-1990 s. The lack of genotyping tools for C. cayetanensis has greatly hampered the cases linkage and population analysis of this pathogen. This study aim to develop a multilocus sequence typing(MLST) tool and analyze population structure of C. cayetanensis. Methods: To identify potential genotyping markers, we sequenced the genome of one C. cayetanensis isolate(CHNEN01) from Henan, China by 454 technology and Illumina technology. Microsatellite and minisatellite repeats were searched from whole genome sequencing date and amplified by nested PCR. Totally, 64 C. cayetanensis-positive specimens, which were from China, Peru, the United States, Nepal, Indonesia and Spain, were used to analyze amplification efficiency and nucleotide sequence polymorphism. Afterwards, population genetics analysis were undertook based MLST data generated from the 64 specimens. Results: Five microsatellite loci(CYC3, CYC13, CYC15, CYC21 and CYC22) exhibiting good amplification and sequence polymorphism were used for genotyping all specimens. Totally, thirty-four specimens had sequence data at five loci, forming 25 multilocus genotypes(MLGs). The linkage disequilibria(LD) in the 64 specimens indicated an overall non-panmictic structure. The disappearance of LD when only considered MLGs confirmed the clonal epidemic structure of the population. Meanwhile, the subpopulation analysis suggested the geographical sub-structuring between Chinese specimens and the other ones mostly from Peru and the United States. Conclusion: The typing tool developed in this study has high resolution and MLST data generated revealed an overall epidemic C. cayetanensis population, which consisting of two remarkable geographical segregated sub-populations. Further analysis of specimens from more geographical regions should be performed to fully understand the transmission dynamics of C. cayetanensis.

关键词： Keywrods Cyclospora cayetanensis Whole genome sequencing multilocus sequence typing Population genetics

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Tracing enterococci persistence along a pork production chain from feed to food in China

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Animal Nutrition 2022年第2期9卷 223-232页

作者： Jianfei Zhao Rui Liu Yanpeng Sun Xiaojun Yang Junhu Yao College of Animal Science and Technology Northwest Agriculture&Forestry UniversityYangling712100China

The prevalence and transmission of vancomycin-resistant Enterococcus(VRE)in enterococci being as probiotics has been neglected in the scientific literature.The application of enterococci in feed,food and health products may cause VRE transmission through the food chain.This study evaluated phenotypic resistance of Enterococcus species to 20 antibiotics along a pork production chain from feed to food.It also assessed the genetic diversity of Enterococcus faecium isolates.A total of 510 samples(feed,n=70;swine manure,n=400;swine carcasses,n=20,and retail pork,n=20)were collected in Beijing,China.A total of 328 enterococci isolates with 275 E.faecium and 53 Enterococcus faecalis were identified using 16 S rRNA.Antimicrobial susceptibility to all enterococci isolates was conducted using the KeB method for 20 antibiotics from 9 categories.multilocus sequence typing(MLST)was conducted on the E.faecium isolates to survey the dissemination of enterococci in the pig industry.The results showed that only 26 enterococci isolates were sensitive to the 20 antibiotics,while half of the isolates(164/328)had acquired multi-drug resistance.The resistant rate to furazolidone was 68.60%,followed by 42.99%to tetracycline.One vancomycin-resistant E.faecium isolates were isolated from feed origin and 2 from manure origin,with minimum inhibitory concentrations to vancomycin of 1,024,64,and 64 mg/mL,respectively.The MLST outcomes showed that the 275 E.faecium isolates belonged to 11 sequence types(ST)including ST40,ST60,ST94,ST160,ST178,ST296,ST361,ST695,ST726,ST812 and ST1014.The ST of the feedsourced VRE was ST1014,while the 2 manure-sourced VRE was ST69.ST1014 evolved from ST78,which was the dominant clonal complex in most cities of China,leading to the spreading of VRE.These findings revealed the potential safety hazards of commercial probiotic enterococci in China and showed that there is a risk of the VRE horizontally transferring from feed to food.

关键词： Antimicrobial resistance multilocus sequence typing Vancomycin-resistant enterococci

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Characterization of the first Wolbachia from the genus Scaptodrosophila,a male-killer from the rainforest species S.claytoni

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Insect Science 2022年第5期29卷 1401-1413页

作者： Kelly M.Richardson Michele Schiffer Perran A.Ross Joshua A.Thia Ary A.Hoffmann School of Biosciences Bio21 InstituteThe University of MelbourneVictoriaAustralia Daintree Rainforest Observatory James Cook UniversityCape TribulationQueenslandAustralia

The Scaptodrosophila genus represents a large group of drosophilids with a worldwide distribution and a predominance of species in Australia,but there is little information on the presence and impacts of Wolbachia endosymbionts in this group.Here we describe the first Wolbachia infection from this group,wClay isolated from Scaptodrosophila claytoni(van Klinken),a species from the east coast of Australia.The infection is polymorphic in natural populations,occurring at a frequency of around 6%–10%.wClay causes male killing,producing female-biased lines;most lines showed 100%male killing,though in 1 line it wasmultilocus sequence typing and the wsp gene.Male-killing Wolbachia are likely to be common in drosophilids but remain difficult to detect because the infections can often be at a low frequency.

关键词： male-killer multilocus sequence typing phenotypic effects Scaptodrosophila Wolbachia

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MLST analysis of genetic diversity of Bacillus coagulans strains to evaluate effects on constipation model

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Food Science and Human Wellness 2022年第4期11卷 815-827页

作者： Jiang Cao Wenyin Liu Ruolan Liliu Jianxin Zhao Hao Zhang Wei Chen Qixiao Zhai State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China National Engineering Research Center for Functional Food Jiangnan UniversityWuxi 214122China Wuxi Translational Medicine Research Center and Jiangsu Translational Medicine Research Institute Wuxi Branch Wuxi 214122China

Bacillus coagulans can help ameliorate or prevent gastrointestinal diseases, but the genetic relationships among B. coagulans isolates are not well studied. multilocus sequence typing analysis was conducted on 57 isolates of B. coagulans from 22 provinces or autonomous regions in China. B. coagulans isolates were highly diverse and a total of 33(sequence typings) STs were found. These isolates had a weak clonal population structure and strong indications of intraspecies recombination. The evolution direction of B. coagulans was not correlated with geography or isolation source. Fifteen strains were selected for further analysis based on proximity relationships from the phylogenetic tree. Five isolates(B. coagulans-1, B. coagulans-10, B. coagulans-39, B. coagulans-70 and B. coagulans-71) with good spore-forming ability relative to the rest of the isolates were evaluated for constipation relief. B. coagulans-39 significantly relieved constipation symptoms in mice by regulating intestinal flora, increasing the production of short-chain fatty acids and restoring the level of gastrointestinal regulatory peptides. Comparative genomic analysis showed the beneficial effects of B. coagulans-39 might be associated with specific functional genes that are involved in the utilization of various carbohydrates as primary substrates and short-chain fatty acid production.

关键词： Bacillus coagulans multilocus sequence typing Housekeeping gene Constipation Strain-specific

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Molecular Characteristics of Neisseria meningitidis Isolated during an Outbreak in a Jail: Association with the Spread and Distribution of ST-4821 Complex Serogroup C Clone in China

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Biomedical and Environmental Sciences 2013年第5期26卷 331-337页

作者： ZHANG Ji ZHOU Hai Jian XU Li HU Guang Chun ZHANG Xue Hua XU Sheng Ping LIU Zun Yu SHAO Zhu Jun Jinan Municipal Center for Disease Control and Prevention State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and Prevention

Objective To characterize the meningococcal strains isolated from cases and close contacts with meningococcal disease associated with an outbreak in a jail in May 2010 by investigating the national distribution of hyperinvasive ST-4821 serogroup C clone associated with this outbreak. Methods The cases were described based on the clinical symptoms and laboratory results. Pharyngeal swabs were cultured for N. meningitidis from men in the jail. Meningococcal isolates were identified by serogrouping, pulsed-field gel electrophoresis （PFGE） and multilocus sequence typing （MLST）, respectively. Four hundred and sixteen serogroup C N. meningitidis strains were collected from 27 provinces between 2003 and 2010 for a nationwide survey and analyzed by PFGE and MLST. Results Three persons in a jail system were infected with invasive N. meningitidis serogroup C. All isolates tested had matching PFGE patterns and belonged to the multilocus sequence type （ST） 4821 clonal complex. All 47 N. meningitidis strains were identified from the pharyngeal swabs of 166 peoples in the jail, and 26 of them belonged to ST-4821 serogroup C clone, and 90.14% （375/416） serogroup C strains identified in the nationwide survey belonged to the ST-4821 complex. The ST-4821 serogroup C clone was spread nationwide, distributed in 24 provinces, especially in eastern provinces between 2003 and 2010. Conclusion Endemic transmission and carriage rate of ST-4821 serogroup C clone are high in this jail system. The ST-4821 serogroup C clone is spreading in China and nationwide distributed despite the existence of some effective vaccines.

关键词： Pulsed-field gel electrophoresis multilocus sequence typing Neisseria meningitidis ST-4821 Serogroup C

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Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections： a multicenter study in China

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Chinese Medical Journal 2011年第5期124卷 687-692页

作者： LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng Department of Respiratory and Critical Care Medicine Peking University People's Hospital Beijing 100044 China Department of Respiratory Medicine Fujian Provincial Hospital Fuzhou Fujian 350001 China Department of Respiratory Medicine Third Affiliated Hospital of the Inner Mongolia Medical College Baotou Inner Mongolia 014010 China Department of Respiratory Medicine First Affiliated Hospital of Nanchang University Nanchang Jiangxi 330006 China Department of Respiratory Medicine Xiangya Hospital of Central South University Changsha Hunan 410008 China Department of Respiratory Medicine Lianyungang First People's Hospital affiliated of Xuzhou Medical College Lianyungang Jiangsu 222002 China Department of Respiratory Medicine Peking University Space Central Hospital Beijing 100049 China Department of Respiratory Medicine Beijing Children's Hospital affiliated of Capital Medical University Beijing 100045 China Department of Emergency Medicine Beijing Children's Hospital affiliated of Capital Medical University Beijing 100045 China Department of Respiratory Medicine Renmin Hospital of Wuhan University Wuhan Hubei 430060 China Department of Respiratory Medicine Nanfang Hospital affiliated to Southern Medical University Guangzhou Guangdong 510515 China Department of Respiratory Medicine Ruijin Hospital affiliated to Shanghai Jiao Tong University Shanghai 200025 China Institute of Respiratory Disease First Affiliated Hospital of China Medical University Shenyang Liaoning 110001 China Department of Respiratory Medicine Third Affiliated Hospital of Guangzhou Medical College Guangzhou Guangdong 510150 China Department of Respiratory Medicine Affiliated Hospital of Guiyang Medical College Guiyang Guizhou 550004 China Department of Respiratory Medicine First Hospital of Lanzhou University Lanzhou Gansu 730000 China Department of Respiratory Medicine First Affiliated Hospital of Guangxi Medical University Nanning Guangxi 530021 China Department of Respiratory Medicin

Background Staphylococcus aureus （S. aureus） remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus in lower respiratory tract infections （LRTIs） have been documented. The aim of this study was to explore the molecular epidemiology of S. aureus in LRTIs in China.Methods A multicenter study of the molecular epidemiology of S. aureus in LRTIs was conducted in 21 hospitals in Beijing, Shanghai and twelve other provinces from November 2007 to February 2009. All the collected S. aureus strains were classified as minimum inhibitory concentration （MIC）, mecA gene, virulence genes Panton-Valentine Leukocidin （PVL） and y-hemolysin （hlg）, staphylococcal cassette chromosome mec （SCCmec） type, agr type, and multilocus sequence Typinq （MLST）.Results Totally, nine methicillin-sensitive S. aureus （MSSA） and 29 methicillin-resistant S. aureus （MRSA） strains were isolated after culture from a total of 2829 sputums or bronchoalveolar lavages. The majority of MRSA strains （22/29） had a MIC value of 〉512 μg/ml for cefoxitin. The mecA gene acting as the conservative gene was carried by all MRSA strains. PVL genes were detected in only one S. aureus strain （2.63%, 1/38）. The hlg gene was detected in almost the all S. aureus （100% in MSSA and 96.56% in MRSA strains）. About 75.86% of MRSA strains carried SCCmec Ⅲ. Agr type 1 was predominant （78.95%） among the identified three agr types （agr types 1,2, and 3）. Totally, ten sequence type （ST） of S. aureus strains were detected. A new sequence type （ST1445） was found besides confirming ST239 as the major sequence type （60.53%）. A dendrogram generated from our own MLST database showed all the bootstrap values 〈50%. Conclusion Our preliminary epidemiology data show SCCmec Ⅲ, ST239 and agr type 1 of S. aureus as the predominant strains in LRTIs in Mainland of China.

关键词： Staphylococcus aureus lower respirato tract infections molecular epidemiology staphylococcal cassette chromosome mec multilocus sequence typing

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Genetic and Antibiotic Resistance Characteristics ofCampylobacterjejuni Isolated from Diarrheal Patients,Poultry and Cattle in Shenzhen

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Biomedical and Environmental Sciences 2018年第8期31卷 579-585页

作者： JU Chang Yan ZHANG Mao Jun MA Yan Ping LU Jing Rang YU Mu Hua CHEN Hui LIU Chu Yun GU Yi Xin FU Yan Yan DUAN Yong Xiang Nanshan Center for Disease Control and Prevention Shenzhen 518054GuangdongChina State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment ofInfectious DiseasesNational Institute for Communicable Disease Control and PreventionChinese Center for DiseaseControl and PreventionBeijing 102206China ORD Environmental Protection AgencyCincinnatiOhio 45220United States

Objective To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni(C. jejuni) isolated from Shenzhen. Methods Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. Results In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat(36.5%) was higher than that in cattle meat(1.1%). However, the prevalence in poultry cloacal swabs(27.0%) was lower than that in cattle stool(57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21(11.9%), ST-22(10.3%), and ST-403(7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin(89.7%), followed by tetracycline(74.6%), and nalidixic acid(69.0%). Conclusion This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry.

关键词： Campylobacter jejuni multilocus sequence typing Antibiotic resistance Poultry Cattle Diarrheal patients

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