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Effects of Pien Tze Huang(片仔癀) on Angiogenesis in vivo and in vitro

Chinese Journal of Integrative Medicine 2012年第6期18卷 431-436页

作者：沈阿灵洪绯刘丽雅林久茂庄群川洪振丰彭军 Academy of Integrative Medicine Biomedical Research Center Fujian University of Traditional Chinese Medicine Fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese Medicine postdoctor Workstation Zhangzhou Pien Tze Huang Pharmaceutical Co.Ltd. Academy of Integrative Medicine Biomedical Research Center Fujian University of Traditional Chinese MedicineFuzhou(350108)China Postdoctor Workstation Zhangzhou Pien Tze Huang Pharmaceutical Co.Ltd.

Objective： TO investigate the anti-angiogenic effects of Pien Tze Huang （片仔癀, PZH） in vivo and in vitro. ***： Human umbilical vein endothelial cells （HUVECs） were treated with 0 mg/mL, 0.25 mg/mL, 0.5 mg/mL, and 1 mg/mL of PZH for 24 h, 48 h and 72 h, respectively. Chicken embryo chorioallantoic membrane （CAM） model was used to evaluate in vivo angiogenesis. An ECMatrix gel system was used to evaluate in vitro angiogenesis by examining the tube formation of HUVECs. 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide assay was performed to determine HUVEC viability. Cell density of HUVECs was observed by phase- contrast microscopy. HUVEC migration was determined by wound healing method. The mRNA and protein expression of vascular endothelial growth factor A （VEGF-A） and basic fibroblast growth factor （bFGF） in both HUVEC and human colon adenocaminoma cells （HT-29） was examined by reverse transcription polymerase chain reaction （RT-PCR） and enzyme linked immune sorbent assay （ELISA）, respectively. Results： PZH treatment significantly reduced the total number of blood vessels compared with the untreated control in the chicken embryos and resulted in a significant decrease in capillary tube formation and cell density of HUVECs （P〈0.05）. In addition, treatment with 0.25-1 mg/mL of PZH for 24 h, 48 h, and 72 h respectively reduced cell viability by 9%-52%, 24%-87% or 25%-87%, compared with the untreated control cells （P〈0.05）. Moreover, PZH treatment decreased the migration of HUVECs. Furthermore, PZH close-dependently suppressed the expression of VEGF-A and bFGF on both mRNA and protein levels （P〈0.05）. Conclusion： PZH could inhibit angiogenesis in vivo in CAM model and in vitro on HUVECs, suggesting that inhibiting tumor angiogenesis might be one of the mechanisms by which PZH treats cancer.

关键词： Pien Tze Huang tumor angiogenesis Chinese medicine multi-target agents

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Virtual Screening of Selective multi-target Kinase Inhibitors by Combinatorial Support Vector Machines

Virtual Screening of Selective Multi-Target Kinase Inhibitor...

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中国化学会第27届学术年会

作者：陈宇综 Bioinformatics and Drug Design Group Department of Pharmacy Center for Computational Science and Engineering National University of Singapore

＜正＞multi-target agents have been increasingly explored for enhancing efficacies and reducing counter-target activities and toxicities. Efficient virtual screening （VS） tools for searching selective multi-target a... 详细信息

关键词： kinase inhibitor SVM multi-target agents

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