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Parent-offspring relationship recognition based on SSR and mtdna confirmed resource supplement effect of Fenneropenaeus chinensis release

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Acta Oceanologica Sinica 2024年第2期43卷 156-160页

作者： Song Sun Ding Lyu Xianshi Jin Xiujuan Shan Weiji Wang Key Laboratory of Sustainable Development of Marine Fisheries Ministry of Agriculture and Rural AffairsYellow Sea Fisheries Research InstituteChinese Academy of Fishery SciencesQingdao 266071China Function Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The resource of Fenneropenaeus chinensis has declined sharply due to excessive fishing intensity,ecological changes and diseases.In order to supplement the fishing yield and restore resources of F.chinensis,the relevant authorities have carried out the activities of stock enhancement and releasing.It can increase biomass and recover resources.However,compared with increasing biomass,there were still few reports on its effect on the recovery of resources.Resource recovery is a process related to whether the released individuals can form a reproductive population.Up to now,there has been a lack of evidence whether the released F.chinensis can complete the entire life history,and form reproduction population.In this study,gravid female shrimp after spawning migration were captured from coastal waters of Haiyang,Qingdao,and Yellow Sea.After identifying parentage relationships using simple sequence repeat(SSR)and mtdna haplotype,it was finally confirmed that there were eight released individuals in the recapture samples.It was confirmed for the first time that at least part of the released F.chinensis can complete overwintering and reproductive migration,and maintain the migration habits as their wild counterparts.Therefore,we infered that the released shrimp can reproduce under natural conditions,these F.chinensis can form reproductive populations theoretically if without human intervention.These results indicated that enhancenment and release activities have a positive effect on resource recovery.

关键词： Fenneropenaeus chinensis release simple sequence repeat(SSR) mtdna resource supplement

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庆阳驴mtdna D-loop区遗传多样性及起源分析

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中国畜牧兽医 2024年第2期51卷 601-613页

作者：施海娜王永杰梁万鹏耿智广李世恩徐振飞刘刚刘哲庆阳市农业科学研究院庆阳745000 宁夏回族自治区水产研究所银川750001 全国畜牧总站北京100193 甘肃农业大学动物科学技术学院兰州730070

[目的]研究庆阳驴养殖群体的遗传多样性与母系起源,了解其遗传信息,为保护庆阳驴种质资源、选育和遗传改良工作提供理论依据。[方法]随机选取133头庆阳驴,对其线粒体DNA(mitochondrial DNA,mtdna)D-loop区序列进行PCR扩增、测序及比对,... 详细信息

[目的]研究庆阳驴养殖群体的遗传多样性与母系起源,了解其遗传信息,为保护庆阳驴种质资源、选育和遗传改良工作提供理论依据。[方法]随机选取133头庆阳驴,对其线粒体DNA(mitochondrial DNA,mtdna)D-loop区序列进行PCR扩增、测序及比对,并探讨庆阳驴的遗传多样性与母系起源。[结果]在获得的520 bp D-loop碱基序列中,AT含量(57.3%)高于GC含量(42.8%),表现出碱基的偏倚性;检测到38个变异位点,包含8个碱基对的转换;其核苷酸多样性(Pi)、单倍型多样性(Hd)、平均核苷酸差异(K)分别为0.01591、0.895和8.274,与欧洲家驴和中国家驴研究的平均值相比较低,说明该驴品种核苷酸变异较为贫乏。庆阳驴mtdna D-loop区存在35个单倍型,单倍型之间的遗传距离为0.002~0.042。系统进化结果显示,庆阳驴存在2个线粒体支系,表明其具有2个母系起源,且遗传距离表明,庆阳驴与克罗地亚家驴之间的遗传距离较近。[结论]本研究从分子水平初步揭示庆阳驴核苷酸变异比较贫乏,杂交程度高,mtdna遗传多态性正逐步丧失,应加强庆阳驴品种的遗传资源保护工作。

关键词：庆阳驴 mtdna D-loop区遗传多样性起源

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Novel Pt(IV) complex OAP2 induces STING activation and pyroptosis via mitochondrial membrane remodeling for synergistic chemo-immunotherapy

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1742-1758页

作者： Renming Fan Ruizhuo Lin Shuo Zhang Aohua Deng Yongrui Hai Junyan Zhuang Yang Liu Maosheng Cheng Gaofei Wei Institute of Medical Research Northwestern Polytechnical UniversityXi'an 710072China Research&Development Institute of Northwestern Polytechnical University in Shenzhen Shenzhen 518057China Key Laboratory of Structure-Based Drug Design and Discovery Ministry of EducationSchool of Pharmaceutical EngineeringShenyang Pharmaceutical UniversityShenyang 110016China

Mitochondrial membrane remodeling can trigger the release of mitochondrial DNA (mtdna), leading to the activation of cellular oxidative stress and immune responses. While the role of mitochondrial membrane remodeling in promoting inflammation in hepatocytes is well-established, its effects on tumors have remained unclear. In this study, we designed a novel Pt(IV) complex, OAP2, which is composed of oxaliplatin (Oxa) and acetaminophen (APAP), to enhance its anti-tumor effects and amplify the immune response. Our findings demonstrate that OAP2 induces nuclear DNA damage, resulting in the production of nuclear DNA. Additionally, OAP2 downregulates the expression of mitochondrial Sam50, to promote mitochondrial membrane remodeling and trigger mtdna secretion, leading to double-stranded DNA accumulation and ultimately synergistically activating the intracellular cGAS-STING pathway. The mitochondrial membrane remodeling induced by OAP2 overcomes the limitations of Oxa in activating the STING pathway and simultaneously promotes gasdermin-D-mediated cell pyroptosis. OAP2 also promotes dendritic cell maturation and enhances the quantity and efficacy of cytotoxic T cells, thereby inhibiting cancer cell proliferation and metastasis. Briefly, our study introduces the first novel small-molecule inhibitor that regulates mitochondrial membrane remodeling for active immunotherapy in anti-tumor research, which may provide a creative idea for targeting organelle in anti-tumor therapy.

关键词： Anti-tumor drug Prodrug Platinum Chemo-immunotherapy Mitochondrial membrane modeling STING Pyroptosis mtdna

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Increased mtdna mutation frequency in oocytes causes epigenetic alterations and embryonic defects

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National Science Review 2022年第10期9卷 67-81页

作者： Longsen Han Yujia Chen Ling Li Chao Ren Haichao Wang Xinghan Wu Juan Ge Wenjie Shu Minjian Chen Qiang Wang State Key Laboratory of Reproductive Medicine Suzhou Municipal Hospital Nanjing Medical University Department of Human Anatomy and Histoembryology Nanjing University of Chinese Medicine Beijing Institute of Microbiology and Epidemiology Key Laboratory of Modern Toxicology of Ministry of Education School of Public Health Nanjing Medical University Center for Global Health School of Public Health Nanjing Medical University

Mitochondria are essential for female reproductive processes,yet the function of mitochondrial D NA(mtdna) mutation in oocytes remains elusive.By employing an mtdna mutator(Polgm) mouse model,we found the fetal growth retardation and placental dysfunction in post-implantation embryos derived from Polgm oocytes.Remarkably,Polgm oocytes displayed the global loss of DNA methylation;following fertilization,zygotic genome experienced insufficient demethylation,along with dysregulation of gene expression.Spindle-chromosome exchange experiment reve aled that cytoplasmic factors in Polgm oocytes are responsible for such a deficient epigenetic remodeling.Moreover,metabolomic profiling identified a significant reduction in the a-ketoglutarate(aKG) level in oocytes from Polgm mice.Importantly,aKG supplement restored both D NA methylation state and transcriptional activity in Polgmembryos,conse quently preventing the developmental defects.Our findings uncover the important role of oocyte mtdna mutation in controlling epigenetic reprogramming and gene expression during embryogenesis.a KG deserves further evaluation as a potential drug for treating mitochondrial dysfunction-related fertility decline.

关键词： mtdna metabolism oocyte embryo epigenetics

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赤水乌骨鸡线粒体DNA控制区遗传多样性和进化分析

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中国畜牧杂志 2024年

作者：吴芸李盼盼闫吉美杨凤黄锡阳遵义师范学院生物与农业科技学院赤水市农业农村局畜牧渔业站

为探究赤水乌骨鸡线粒体DNA控制区的遗传多样性、单倍型特征和母系起源情况，利用PCR扩增和直接测序法检测了63只赤水乌骨鸡线粒体DNA （mitochondrial DNA， mtdna）控制区（D-loop）全序列，分析其遗传多样性和单倍型特性，并与不同... 详细信息

为探究赤水乌骨鸡线粒体DNA控制区的遗传多样性、单倍型特征和母系起源情况，利用PCR扩增和直接测序法检测了63只赤水乌骨鸡线粒体DNA （mitochondrial DNA， mtdna）控制区（D-loop）全序列，分析其遗传多样性和单倍型特性，并与不同红色原鸡亚种的序列进行聚类分析。结果表明，赤水乌骨鸡mtdna D-loop区全序列共检测到29个突变位点，构成19种单倍型。遗传多样性分析表明，单倍型多样性（Hd）、核苷酸多样性（Pi）和平均核苷酸差异数（K）分别为0.990 5±0.018 0、0.006 5±0.000 5和7.981。系统发育分析显示，赤水乌骨鸡19种单倍型可划分为A、B、C、E、G 5个单倍型群，A、B均分别与原鸡滇南亚种聚类，单倍型群C与原鸡印度亚种、原鸡滇南亚种和原鸡海南亚种聚为一类，E单倍型群分支与印度亚种和原鸡滇南亚种聚为一类。据此推测，赤水乌骨鸡遗传多样性丰富，可能有多个母系起源，主要源于原鸡滇南亚种。

关键词：赤水乌骨鸡 mtdna 控制区遗传多样性系统进化

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传明酸多肽组合物改善线粒体功能及炎性色沉的研究

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日用化学品科学 2024年第6期47卷 13-18页

作者：王琪郑含含张肖黄琳王晓东卢云宇上海优斐斯生物科技有限公司上海201403 杭州彗柏生物科技有限公司浙江杭州311200

利用4种细胞模型,研究了单用传明酸及传明酸+精氨酸/赖氨酸多肽+谷胱甘肽(传粒亮TXATidePro™)组合物对人皮肤成纤维细胞(HFF-1)细胞代谢活性及线粒体形态学水平的影响;对人角质形成细胞(HaCaT)ATP生成、mtdna拷贝数及细胞自噬水平的影响... 详细信息

利用4种细胞模型,研究了单用传明酸及传明酸+精氨酸/赖氨酸多肽+谷胱甘肽(传粒亮TXATidePro™)组合物对人皮肤成纤维细胞(HFF-1)细胞代谢活性及线粒体形态学水平的影响;对人角质形成细胞(HaCaT)ATP生成、mtdna拷贝数及细胞自噬水平的影响;对RAW264.7细胞TNF-α及IL-1β水平的影响;以及对B16F10细胞黑色素水平的影响。结果表明,传粒亮TXATidePro™组合物较对照组及单用传明酸显著促进细胞代谢活性,改善线粒体质量,提升ATP生成,降低mtdna损伤,促进细胞自噬,抑制TNF-α及IL-1β,降低黑色素含量。传粒亮TXATidePro™组合物可通过改善皮肤细胞线粒体及自噬功能达到抗炎、提亮淡斑及抵抗炎性色沉的作用。

关键词：传明酸精氨酸/赖氨酸多肽谷胱甘肽线粒体 mtdna 细胞自噬炎性色沉黑色素

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Diet induced the change of mtdna copy number and metabolism in Angus cattle

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Journal of Animal Science and Biotechnology 2021年第1期12卷 172-184页

作者： Ying Bai José A.Carrillo Yaokun Li Yanghua He Jiuzhou Song College of Life Sciences and Food Engineering Hebei University of EngineeringHandan 056038China .2Department of Animal&Avian Sciences University of MarylandCollege ParkMD 20742USA Council on Dairy Cattle Breeding BowieMD 20716USA .4Human Nutrition Food and Animal SciencesUniversity of Hawaii at ManoaHonoluluHI 96822USA.

Background: Grass-fed and grain-fed Angus cattle differ in the diet regimes. However, the intricate mechanisms of different beef quality and other phenotypes induced by diet differences are still unclear. Diet affects mitochondrial function and dynamic behavior in response to changes in energy demand and supply. In this study, we examined the mtdna copy number, mitochondria-related genes expression, and metabolic biomarkers in grass-fed and grainfed Angus cattle.Results: We found that the grass-fed group had a higher mtdna copy number than the grain-fed group. Among different tissues, the mtdna copy number was the highest in the liver than muscle, rumen, and spleen. Based on the transcriptome of the four tissues, a lower expression of mtdna-encoded genes in the grass-fed group compared to the grain-fed group was discovered. For the mitochondria-related nuclear genes, however, most of them were significantly down-regulated in the muscle of the grass-fed group and up-regulated in the other three tissues. In which, COX6 A2, POLG2, PPIF, DCN, and NDUFA12, involving in ATP synthesis, mitochondrial replication,transcription, and maintenance, might contribute to the alterations of mtdna copy number and gene expression.Meanwhile, 40 and 23 metabolic biomarkers were identified in the blood and muscle of the grain-fed group compared to a grass-fed group, respectively. Integrated analysis of the altered metabolites and gene expression revealed the high expression level of MDH1 in the grain-fed group might contribute to the mitochondrial NADH oxidation and spermidine metabolism for adapting the deletion mtdna copy number.Conclusions: Overall, the study may provide further deep insight into the adaptive and regulatory modulations of the mitochondrial function in response to different feeding systems in Angus cattle.

关键词： Beef quality Different diet Gene expression Metabolism mtdna

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Mitochondrial DNA Polymorphism in Natural Populations of Drosophila albomicans (Ⅰ)——Remarkable mtdna Polymorphism in the Population of D.albomicans

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Science China Chemistry 1994年第11期37卷 1329-1340页

作者：王文凌发瑶施立明 Laboratory of Cellular and Molecular Evolution Kunming Institute of Zoology Academia SinicaKunming 650223PRC Laboratory of Cellular and Molecular Evolution Kunming Institute of ZoologyAcademia SinicaKunming 650223PRC

The technique of mtdna restriction fragments length polymorphism(RFLP)was used tosurvey the population structure of D.albomicans.Remarkable mtdna polymorphism has been observed inD.albomicans populations.A total of 34 nucleomorphs were detected from 82 isofemale lines assayed by only8 restriction enzymes.The cause and the effect of this phenomenon were discussed.As a result,it is sug-gested that a mechanism which maintains mtdna diversity exists in this fly,and that the high intra-popula-tional polymorphism could numerically conceal the extent of differentiation between populations.In addition,on the base of restriction maps,it was found that the mtdna molecule of D.albomicans might be impactedby the selection pressure during its evolution process both on the nucleotide composition and on the function-al regions.

关键词： Drosophila albomicans natural population restriction maps mtdna polymorphism

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Sequence-length variation of mtdna HVS-I C-stretch in Chinese ethnic groups

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第10期10卷 711-720页

作者： Feng CHEN Yong-hui DANG Chun-xia YAN Yan-ling LIU Ya-jun DENG David J. R. FULTON Teng CHEN Department of Forensic Medicine School of Medicine Xi'an Jiaotong University Xi'an 710061 China Ministry of Education Key Laboratory of Environment and Genes Related to D&eases Xi 'an Jiaotong University Xi'an 710061 China Beijing Genomics Institute Chinese Academy of Sciences Beijing 100029 China Medical College of Georgia 1459 Laney Walker Blvd Augusta Georgia 30912 USA

The purpose of this study was to investigate mitochondrial DNA (mtdna) hypervariable segment-I (HVS-I) C-stretch variations and explore the significance of these variations in forensic and population genetics studies. The C-stretch sequence variation was studied in 919 unrelated individuals from 8 Chinese ethnic groups using both direct and clone sequencing ap-proaches. Thirty eight C-stretch haplotypes were identified, and some novel and population specific haplotypes were also detected. The C-stretch genetic diversity (GD) values were relatively high, and probability (P) values were low. Additionally, C-stretch length heteroplasmy was observed in approximately 9% of individuals studied. There was a significant correlation (r=-0.961, P<0.01) between the expansion of the cytosine sequence length in the C-stretch of HVS-I and a reduction in the number of up-stream adenines. These results indicate that the C-stretch could be a useful genetic maker in forensic identification of Chinese populations. The results from the Fst and dA genetic distance matrix, neighbor-joining tree, and principal component map also suggest that C-stretch could be used as a reliable genetic marker in population genetics.

关键词：线粒体DNA 拉伸长度序列长度 HVS 群体遗传学华裔法医鉴定 mtdna

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The phylogeny of Orthoptera inferred from mtdna and description of Elimaea cheni(Tettigoniidae:Phaneropterinae)mitogenome

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Journal of Genetics and Genomics 2010年第5期37卷 315-324页

作者： Zhijun Zhou Haiyan Ye Yuan Huang Fuming Shi a College of Life Science Hebei University Baoding 071002 China College of Life Science Shaanxi Normal University Xi'an 710062 China

The complete 15,831 bp nucleotide sequence of the mitochondrial genome from Elimaea cheni （Phaneropterinae） was determined. The putative initiation codon for cox1 was TTA. The phylogeny of Orthoptera based on different mtdna datasets were analyzed with maximum likelihood （ML） and Bayesian inference （BI）. When all 37 genes （mtdna） were analyzed simultaneously, the monophyly of Caelifera and Ensifera were recovered in the context of our taxon sampling. The phylogeny of Orthoptera was largely consistent with previous phylogenetic hypotheses. Rhaphidophoridae to be a sister group of Tettigoniidae, and the relationships among four subfamilies of Tettigoniidae were （Phaneropterinae ＋（Conocephalinae ＋（Bradyporinae ＋ Tettigoniinae）））. Pyrgomorphidae was the most basal group of Caelifera. The relationships among six acridid subfamilies were （Oedipodinae ＋（Acridinae ＋（Gomphocerinae ＋（Oxyinae ＋（Callip- taminae ＋ Cyrtacanthacridinae）））））. However, we did not recover a monophyletic Grylloidea. Myrmecophilidae clustered into one clade with Gryllotalpidae instead of with Gryllidae. ML and BI analyses of all protein coding genes （using all nucleotide sequence data or excluding the third codon position, and amino acid sequences） revealed a topology identical to that of the entire mtdna genome dataset. However, 22 tRNAs genes excluding the DHU loop and TψC loop （TRNA）, and two rRNA genes （RRNA） perform poorly when analyzed as single dataset. Our results suggest that the best phylogenetic inferences were ML and BI methods based on total mtdna. Excluding tRNA genes, rRNA genes and the third codon position of protein coding genes from dataset and converting nucleotide sequences to amino acid sequences do not positively affect phylogenetic reconstruction.

关键词： mtdna mitogenome Elimaea cheni Orthoptera phylogeny

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