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Study on the Vesiculation during mouse sperm Acrosome Reaction

Journal of Reproduction and Contraception 1994年第2期5卷 265-269页

作者：沙家豪周作民胡志刚王黎熔林敏张适

The location Of the mono-membrane and the bi-membrane vesicles of mouse sperm wasidentified using Con A in conjugation with the colloidal gold. The observation showed that bothmono-membrane vesicles and outer layer of the bi-membrane vesicles come from the outeracrosome *** inner membrane layer of the bi-member vesicles and residual membrane distributed among the vesicles are really foe *** is suggested that the outeracrosome membrane did not fuse with the plasmalemma during mouse sperm acrosome reaction and that both the mono-membrane and the bi-membrane vesicles of mouse sperm wereformed due to winding of the outer acrosome membrane.

关键词： mouse sperm Bi-membrane vesicles,Con A,Colloid gold

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Vlouse sperm acquire a new structure on the apical hoo Juring epididymal maturation

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Asian Journal of Andrology 2013年第4期15卷 523-528,I0009页

作者： Yi-Wen Lin Tzu-Han Hsu Pauline H Yen lnstitute of Biomedical Sciences Academia Sinica Taipei 11529 China and lnstitute of Cellular and Organismic Biology Academia Sinica Taipei 11529 China

spermatozoa emerging from the testis undergo a maturation process in the epididymis during which they change morphologically, biochemically and physiologically to gain motility and the ability to fertilize ova. We examined mouse epididymal sperm with immunostaining and transmission electron microscopy （EM） and identified a previously unknown structure on the apical hook. The structure has a coiled configuration around 11 nm in thickness and is present at the tip of each corner of the triangular-rod shaped perforatorium. Surveying sperm isolated from various regions of the epididymis indicated that mouse sperm acquire the hook rim （HR） structure during its passage through the proximal two-thirds of the caput epididymidis. The structure withstands vigorous sonication and harsh chemical treatments and remains intact after the acrosome reaction. Its location and sturdiness suggest a function in protecting the apical hook from mechanical wear during fertilization. Our EM images of epididymal sperm also revealed additional novel structures as well as lateral asymmetry of the sperm head, indicating that mouse sperm head has a structure more complex than previously recognized.

关键词： apical hook epididymal maturation mouse sperm sperm head ultrastructure

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The opening of maitotoxin-sensitive calcium channels induces the acrosome reaction in human spermatozoa： differences from the zona pellucida

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Asian Journal of Andrology 2011年第1期13卷 159-165页

作者： lulio C Chaivez Gerardo A de Blas Josd L de la Vega-Beltran Takuya Nishigaki Mayel Chirinos Maria Elena Gonzaez-Gonzalez Fernando Larrea Alejandra Soiis Alberto Darszon Claudia L Trevino Department of Developmental Genetics and Molecular Physiology Biotechnology Institute UNAM Cuernavaca Mexico Department of Reproduction Biology National Institute of Medical Sciences and Nutrition ＇Salvador Zubiran＇ Mexico City Mexico

The acrosome reaction （AR）, an absolute requirement for spermatozoa and egg fusion, requires the influx of Ca2＋ into the spermatozoa through voltage-dependent Ca2＋ channels and store-operated channels. Maitotoxin （MTx）, a Ca2＋-mobilizing agent, has been shown to be a potent inducer of the mouse sperm AR, with a pharmacology similar to that of the zona pellucida （ZP）, possibly suggesting a common pathway for both inducers. Using recombinant human ZP3 （rhZP3）, mouse ZP and two MTx channel blockers （U73122 and U73343）, we investigated and compared the MTx- and ZP-induced ARs in human and mouse spermatozoa. Herein, we report that MTx induced AR and elevated intracellular Ca2＋（[Ca2＋]~） in human spermatozoa, both of which were blocked by U73122 and U73343. These two compounds also inhibited the MTx-induced AR in mouse spermatozoa. In disagreement with our previous proposal, the AR triggered by rhZP3 or mouse ZP was not blocked by U73343, indicating that in human and mouse spermatozoa, the AR induction by the physiological ligands or by MTx occurred through distinct pathways. U73122, but not U73343 （inactive analogue）, can block phospholipase C （PLC）. Another PLC inhibitor, edelfosine, also blocked the rhZP3- and ZP-induced ARs. These findings confirmed the participation of a PLC-dependent signalling pathway in human and mouse zona protein-induced AR. Notably, edelfosine also inhibited the MTx-induced mouse sperm AR but not that of the human, suggesting that toxin-induced AR is PLC-dependent in mice and PLC-independent in humans.

关键词： acrosome reaction calcium channels human sperm maitotoxin mouse sperm

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