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Protective Effects of Saponin Mixture, Isolated from <i>Astragalus monspessulanus</i>subsp. <i>monspessulanus</i>on Tert-Butyl Hydroperoxide—Induced Oxidative Stress in Isolated Rat Hepatocytes

引用

American Journal of Plant Sciences 2015年第6期6卷 799-803页

作者： Magdalena Spasova Kondeva-Burdina Viktor Bratkov Rumyana Lubomirova Simeonova Vessela Bisserova Vitcheva Ilina Nikolaeva Krasteva Petranka Krumova Zdraveva Laboratory of Drug Metabolism and Drug Toxicity Department of Pharmacology Pharmacotherapy and Toxicology Medical University of Sofia Sofia Bulgaria Department of Pharmacognosy Faculty of Pharmacy Medical University of Sofia Sofia Bulgaria

Saponin mixture, obtained from Astragalus monspessulanus subsp. monspessulanus (Fabaceae) was investigated for possible protective effect on tert-butyl hydroperoxide (t-BuOOH)-induced cytotoxicity using primary isolated rat hepatocytes. The cells were isolated by two-stepped col-lagenase perfusion. Liver damage was induced by one hour incubation with t-BuOOH (75 μmol·L-1) and discerned by decreased cell viability, increased lactate dehydrogenase (LDH) leakage into the medium, increased production of malondialdehyde (MDA) and depletion of the cell protector glutathione (GSH). Cell pre-incubation with the saponin mixture (1 mg/mL and 5 mg/mL) significantly (p < 0.05) ameliorated t-BuOOH-induced liver damage, judged by preserved cell viability, decreased activity of LDH, decreased MDA production and restoration of GSH. The effect was concentration-dependent, more pronounced in the highest concentration and comparable with those of silymarin, used as a positive control. The observed cytoprotective effect could be explained by the influence of the saponins on the mitochondrial function, disturbed by t-BuOOH toxic metabolites.

关键词： Astragalus monspessulanus Saponins Hepatocytes Antioxidant Activity Cytoprotection

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