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Effects of moniliformin and selenium on human articular cartilage metabolism and their potential relationships to the pathogenesis of Kashin-Beck disease

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第3期11卷 200-208页

作者： An ZHANG Jun-ling CAO Bo YANG Jing-hong CHEN Zeng-tie ZHANG Si-yuan LI Qiang FU Clare E. HUGNES Bruce CATERSON Institute of Endemic Diseases School of Medicine Xi 'an Jiaotong University Xi 'an 710061 China Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education Xi 'an Jiaotong University Xi 'an 710061 China Key Laboratory of Microelement and Endemic Disease of Ministry of Health Xi 'an Jiaotong University Xi'an 710061 China Laboratory of Connective Tissue Biology School of Biosciences Cardiff University Cardiff CF10 3US UK

Objective： To investigate the effects of mycotoxin moniliformin （MON） on the metabolism of aggrecan and type 11 collagen in human chondrocytes in vitro and the relationship between MON and Kashin-Beck disease （KBD）. Methods： Human chondrocytes were isolated and cultured on bone matrix gelatin to form an artificial cartilage model in vitro with or without MON toxin. Cell viability was determined by 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide （MTT） assay. The expression of aggrecan and type II collagen in the cartilage was determined using immunocytochemical staining. Results： MON toxin inhibited chondrocyte viability in dose-dependent and time-dependent manners. MON reduced aggrecan and type Ⅱ collagen syntheses in the tissue-engineered cartilage. MON also increased the expression of matrix metalloproteinase-1 （MMP-1）, MMP-13, BC4 epitopes, and CD44 in cartilages. However, the expression of 3B3（-） epitopes in cartilages was inhibited by MON. Selenium partially alleviated the damage of aggrecan induced by MON toxin. Conclusion： MON toxin promoted the catabolism of aggrecan and type II collagen in human chondrocytes.

关键词： Chondrocytes moniliformin Selenium Aggrecan Collagen Matrix metalloproteinases (MMPs) CD44 Kashin-Beck disease

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Detoxifying moniliformin in grains and water

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Journal of Environmental Sciences 1997年第2期9卷 90-95页

作者： Zhang Hong Li Jilun College of Biological Sciences China Agricultural UniversityBeijing 100094China

The method to determine moniliformin content was established in our laboratory. The recovery rate is 97 7% while the moniliformin content in the sample is 8 8 mg/g. In comparison with different methods of detoxifying moniliformin in water, the best antidote is chlorinated lime. 1 5 mg active chlorine in chlorinated lime was required to detoxify 1 mg moniliformin. 5% H 2O 2 spray was the best way for detoxifying moniliformin in grains. These two methods are convenient, economic and with no secondary pollution. They can be used for preventing human and livestock from the toxicity of moniliformin.

关键词： moniliformin detoxifying Keshan disease.

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THE EXPERIMENTAL STUDY ON THE EFFECTS OF SIX MYCOTOXINS ON THE CULTURAL CHONDROCYTES

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Journal of Pharmaceutical Analysis 1998年第1期12卷 1-8页

作者：曹峻岭熊咏民李斯纯郑滨张矢远毕华银莫东旭 Institute of Endemic Bone Diseases Xi'an Medical University Xi'an 710061 China

The effects of deoxynlvalenol (DON), T--2 toxin, nivalenol (NIv), hutenolide (BuT).alternariol methyl ether(AME) and monlliformin (cON ) on rabbit articular chondrocytes were observed by using the method of chondrocyte monolayer culture. The amounts or DNA in chondrocytesand glucuronate in matrix were measured. And the chondrocytes were observed by inversion microscope and transmission electron microscope (TEM). The results showed that the cultured chondrocytes were damaged by all the six mycotoxinsl and the synthesis of DNA and the divided reproductionof chondrocytes were restrained; the damage errect was more evident, esl,ecially in the early stage ofculturel the higher concentration or toxin in the media was used, the lower density of the culturalckondrocytes was observed; the cells were even round damaged and dead, so long as the media contolued toxin. When the six mycotoxins arrected the cultural chondrocytes ***, three dirfereut kinds or ultrastructural changes in ckondrocytes were seen by TEa. The relationship betweenmycotoxiu and KBD was preliminarily discussed, and some problems still need further investigation..

关键词： chondrocyte culture deoxynivalenol T-2 toxin nivalenol butenolide alternariol methyl ether moniliformin Kashin-Beck disease

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Mango Malformation: I. Toxin Production Associated with Fusarium Pathogens

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American Journal of Plant Sciences 2011年第2期2卷 276-281页

作者： Wafaa Haggag M. Hazza M. Sehab A. Abd El-Wahab M. 不详

Eight Fusarium species i.e. F. subglutinans, F. solani, F. oxyspoum, F. sterilihyphosum, F. proliferatum, F. monili-forme, F. avena and F. chlamydspore isolated from mango malformed disease were tested for their ability to cause mango malformation disease and their production of moniliformin and total fumonisins (FB1 + FB2) using HPLC. A evaluated for moniliformin production, seven isolates were toxin producers, the production levels ranging from 0.51 to 8.90 μg/ml. The higher levels were produced by Fusarium subglutinans (8.51 μg/ml). Moderate concentrations of moniliformin was produced by *** (6.90 μg/ml), F. oxysporum (6.30 μg/ml), F. proliferatum (4.10 μg/ml) and F. sterilihyphosum (1.10 μg/ml). Separation and identification of Fumonisin that was isolated from the pathogen- causing disease are made by (HPLC). A evaluated for total fumonisin production (FB1 + FB2), seven isolates were toxin producers, the production levels ranging from 0.10 to 8.30 μg/ml. The higher levels were produced by F. monili-forme (8.30 μg/ml. Moderate concentrations of fumonisin was produced by F .proliferatum (0.64 μg/ml) and F. subglutinans (0.50 μg/ml). Strong positive correlations between moniliformin and total fumonisins (FB1 + FB2) activities and malformation disease incidence by F. subglutinans, F. solani, F. oxyspoum, F. sterilihyphosum, F. proliferatum was observed.

关键词： Fusarium Mango Malformation moniliformin And Fumonisins

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