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Genetic and genomic analyses for economically important traits and their applications in molecular breeding of cultured fish

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Science China(Life Sciences) 2015年第2期58卷 178-186页

作者： TONG JinGou SUN XiaoWen State Key Laboratory of Freshwater Ecology and Biotechnology Institute of Hydrobiology Chinese Academy of Sciences Heilongjiang Fisheries Research Institute Chinese Academy of Fishery Sciences

The traits of cultured fish must continually be genetically improved to supply high-quality animal protein for human *** important fish traits are controlled by multiple gene quantitative trait loci(QTL),most of which have minor effects,but a few genes may have major effects useful for molecular *** this review,we chose relevant studies on some of the most intensively cultured fish and concisely summarize progress on identifying and verifying QTLs for such traits as growth,disease and stress resistance and sex in recent *** potential applications of these major-effect genes and their associated markers in marker-assisted selection and molecular breeding,as well as future research directions are also *** genetic and genomic analyses will be valuable for elucidating the mechanisms modulating economically important traits and to establish more effective molecular breeding techniques in fish.

关键词： aquaculture fish economic traits QTL major genes molecular markers molecular breeding

Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding

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The Crop Journal 2018年第4期6卷 366-376页

作者： Lin Sun Muna Alariqi Yi Zhu Jianying Li Zelin Li Qing Wang Yajun Li Hangping Rui Xianlong Zhang Shuangxia Jin National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 Hubei China Department of Agronomy and Pastures Faculty of Agriculture Sana'a University Yemen

Genes encoding reporter proteins are used as visual marker-assisted tools in genetic transformation as well as plant breeding. In this study, the red fluorescent protein identified in Discosoma sp. coral(DsRed2) was successfully used as a visual marker for cotton genetic engineering. DsRed2 was successfully expressed in two cotton cultivars,JIN668 and YZ1, driven by the Ca MV-35 S promoter via the Agrobacterium-mediated transformation. Our results suggest that DsRed2 expression provides an early-stage selection tool for the transgenic calli via visual observation. Red fluorescence can be detected not only in callus and somatic embryos but also in most tissues and organs of mature plants. The transgenic line Yz-2-DsRed2 was crossed with four different cotton cultivars to assess the transgene heritability and stability in different genetic *** heritability of the red color was highly stable when Yz-2-DsRed2 was used as a male parent. The DsRed2 gene expressed 100% in the F_1 hybrids. To investigate the relationship between DsRed2 transcription and DNA methylation, a methylation-specific PCR approach was applied to the Ca MV-35 S promoter region. The results showed a negative association between DNA methylation level in the promoter region and the transgene *** together, these findings suggest DsRed2 a visual reporter gene for cotton genetic transformation and molecular breeding programs.

关键词： DsRed2 Reporter gene Transgenic cotton molecular breeding DNA methylation

molecular breeding of farm animals through gene editing

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National Science Open 2023年第5期2卷 75-95页

作者： Fei Gao Naipeng Hou Xuguang Du Yu Wang Jianguo Zhao Sen Wu State Key Laboratory of Animal Biotech breeding College of Biological SciencesNational Engineering Laboratory for Animal BreedingFrontiers Science Center for Molecular Design BreedingChina Agricultural UniversityBeijing 100193China College of Animal Science and Technology China Agricultural UniversityBeijing 100193China Sanya Institute of China Agricultural University Sanya 572025China Institute of Zoology Chinese Academy of SciencesBeijing 100101China

The rapid development of biotechnology has facilitated our understanding of the biological functions of candidate genes for important economic traits in farm *** breeding by gene editing has greatly revolutionized the breeding of farm *** gene editing and embryo manipulation,breeds with designed economic or disease-resistant traits can be readily *** with this fast progress,the safety assessment of gene-edited farm animals has attracted public and regulatory *** review summarizes the research progress of gene editing in farm animals,focusing on performance improvement,disease resistance,bioreactors,animal welfare,and environmental *** limitations and future development of gene editing technology in farm animal breeding are also discussed.

关键词： molecular breeding farm animals gene editing

Prospect of the QTL-qSB-9^(Tq) utilized in molecular breeding program of japonica rice against sheath blight

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Journal of Genetics and Genomics 2008年第8期35卷 499-505页

作者： Shimin Zuo Li Zhang Hui Wang Yuejun Yin Yafang Zhang Zongxiang Chen Yuyin Ma Xuebiao Pan Key Laboratory of Crop Genetics and Physiology of Jiangsu Province Yangzhou University Yangzhou 225009 China Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

The major QTL-qSB-9^Tq conferring partial resistance to rice （Oryza sativa L.） sheath blight （Rhizoctonia solani Kvhn） has been verified on chromosome 9 of the indica rice cultivar, Teqing. In this study, the prospect of this QTL utilized in molecular breeding program of japonica rice for sheath blight resistance was investigated. Most of the japonica rice cultivars showed lower level of sheath blight resistance than the indica rice cultivars. At the corresponding site of qSB-9^Tq, nine typical japonica rice cultivars from different ecological regions or countries proved to possess the susceptible allele（s）. Introgression of qSB-9^Tq into these cultivars enhanced their resistance level by decreasing sheath blight score of 1.0 （0.5-1.3）, which indicated that qSB-9^Tq had a large potential in strengthening the resistance of japonica rice to sheath blight. The use of the three molecular markers, which were polymorphic between Teqing and many japonica rice cultivars, promotes the application of qSB-9^Tq in a concrete molecular breeding program.

关键词： rice (Oryza sativa L.) japonica cultivar sheath blight QTL molecular breeding

molecular breeding of hybrid rice

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Hunan Agricultural Science ＆ Technology Newsletter 2003年第2期4卷 8-12页

作者： Wang Xiusong1. College of Agronomy, Hunan Agricultural University, Changsha 410128, China2. Yuan Longping High-Tech Agriculture Co., Ltd., Changsha 410125, China College of Agronomy Hunan Agricultural University Changsha 410128 China Yuan Longping High - Tech Agriculture Co. Ltd. Changsha 410125 China

This paper describes in a general way the objectives, contents and methods of molecular breeding, and the application of molecular breeding in hybrid rice.

关键词： molecular breeding plant gene engineering total foreign DNA introduction hybrid rice

molecular breeding and Economic Importance of Pomegranate:A Review

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Molecular Breeding and Economic Importance of Pomegranate:A ...

Present Status, Problems and Prospects of molecular breeding of Cotton (Gossypium) with Resistance against Verticillium dahliae Kleb.

第四届中国石榴博览会暨第十届全国石榴生产与科研研讨会、首届国际石榴文化数字云博览会

作者： Krishna Poudel CHEN Lina LI Haoxian CAO Shangyin Zhengzhou Fruit Research Institute

Pomegranate is native to central Asia,which is an important fruit contributing to poverty alleviation and rural *** strategies followed by most of the breeders for breeding new varieties are based on the controlled crosses to select those individuals with desired traits of *** is one of the successful approaches in the production of most of the crop varieties available *** the fact,this process of breeding in fruit crops is time-consuming and costly due to the time required by the fruit crop to enter into the fruiting *** development in science as sequencing and mapping techniques are the new scientific tools for breeding in fruit *** breeding strategies have mostly focused on the introduction to the high yield and/or resistance-related traits to *** traits in pomegranates are the basic criteria for the consumers in selecting quality *** of the major traits representing the quality of the products are fruit color,seed hardness,aril color,taste,*** edibility of the fruits of pomegranate depends on the fleshy outer seed coats and hard or soft inner seed *** is the key factor for the preferences by the consumers to particular *** consumers’ preference towards softseeded pomegranates has accelerated the breeding of new soft-seeded pomegranate ***,the seed coat development in pomegranate has not yet been *** hardness is an important trait in pomegranate that determines the edibility and quality of the *** is a major factor for the economic value of the fruit as soft seeded fruits fetch a higher price in the market compared to the hard seeded ones.

关键词： Pomegranate Seed hardness molecular breeding Marker Genetic diversity

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Plant Diseases and Pests 2011年第5期2卷 1-5,10页

作者：汪保华高灵路王长彪南通大学生命科学学院江苏南通226019 山西省农业科学院棉花研究所山西运城044000

VerticiUium wilt is an important disease in cotton production, which seriously affects the production of cotton. The classification and pathogenesis of Yer-ticillium dahliae Kleb. , achievements in QTL mapping and molecular breeding were reviewed in the paper. Meanwhile, the current problems and future direction of resistance breeding against V. dahliae Kleb. were discussed so as to provide reference information for further research on resistance breeding against the pathogen.

关键词： Cotton Verticillium wilt QTL molecular breeding China

High-through screening of salt-alkaline tolerance genes based on gene microarray and RNA-sequencing and its application to molecular breeding in Glycine soja

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High-through screening of salt-alkaline tolerance genes base...

Great progress in molecular breeding of hybrid rice in CNHRRDC

第十四届全国植物基因组学大会

作者： Huizi DuanMu Xiaoli Sun Chao Chen Shufei Cheng Yang Yu Yanming Zhu Plant Bioengineering Laboratory Northeast Agricultural University

Soil salinity-alkalinity is one of the major environmental challenges limiting crop productivity *** soja is considered to be an ideal material for the research of stress-related gene cloning and molecular mechanism r... 详细信息

关键词： Glycine soja alkaline stress gene transcript profiling function analyzing screening of key genes molecular breeding

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Agricultural Science & Technology 2005年第3期6卷 2-6页

作者：赵炳然袁隆平国家杂交水稻工程技术研究中心长沙湖南410125

Elite maintainer lines and restorer lines have been developed by genomic DNA transformation,Analyses of molecular markers, DNA sequences, and Southern blotting have revealed that high DNA polymorphism exists between new developed lines and its receptors, indicating that the special DNA fragment from distant relatives may be integrated into the genome of rice. And several combinations with the potential of super-high yield have been developed from these lines. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for breeding of super-hybrid rice.

关键词： Super-hybrid rice molecular breeding genomic DNA transformation germplasm