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Journal of Environmental Sciences 2010年第5期22卷 796-800页

作者： Qiyan Ye Huisheng Zhuang Chun Zhou Qiong'e Wang College of Environmental Science and Engineering Donghua University Shanghai 201620 China School of Environmental Science and Engineering Shanghai Jiao Tong University Shanghai 200240 China College of Chemistry Chemical Engineering and Biotechnology Donghua University Shanhai 201620 China

A reliable and sensitive competitive real-time fluorescent quantitative immuno-PCR （RTFQ-IPCR） assay using a molecular beacon was developed for the determination of trace fluoranthene （FL） in the *** optimized assay conditions,FL can be determined in the concentration range from 1 fg/mL to 100 ng/mL,with y=0.194x ＋ 7.859,and a correlation coefficient of 0.967 was identified,with a detection limit of 0.6 fg/*** water samples were successfully analyzed,recovery was between 90% and 116%,with intra-day relative standard deviation （RSD） of 6.7%-12.8% and inter-day RSD of 8.4%-15.2%.The results obtained from RTFQ-IPCR were confirmed by ELISA,showing good accuracy and suitability to analyze FL in field *** a highly sensitive method,the molecular beacon-based RTFQ-IPCR is acceptable and promising for providing reliable test results to make environmental decisions.

关键词： fluoranthene real-time fluorescent quantitative irnmuno-PCR molecular beacon

Engineering Novel molecular beacon Constructs to Study Intracellular RNA Dynamics and Localization

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Genomics, Proteomics & Bioinformatics 2017年第5期15卷 279-286页

作者： Zhao Ma Xiaotian Wu Christopher J.Krueger Antony K.Chen Department of Biomedical Engineering College of Engineering Peking University Beijing 100871 China Wallace H Coulter Department of Biomedical Engineering Georgia Institute of Technology Atlanta GA 30332 USA

With numerous advancements in novel biochemical techniques, our knowledge of the role of RNAs in the regulation of cellular physiology and pathology has grown significantly over the past several decades. Nevertheless, detailed information regarding RNA processing, trafficking, and localization in living cells has been lacking due to technical limitations in imaging single RNA transcripts in living cells with high spatial and temporal resolution. In this review, we discuss tech- niques that have shown great promise for single RNA imaging, followed by highlights in our recent work in the development of molecular beacons （MBs）, a class of nanoscale oligonucleotide-probes, for detecting individual RNA transcripts in living cells. With further refinement of MB design and development of more sophisticated fluorescence microscopy techniques, we envision that MB-based approaches could promote new discoveries of RNA functions and activities.

关键词： Y-O-methyl RNA Phosphorothioate molecular beacon RNA dynamics Single-molecule RNAimaging

Ligase-based ultrasensitive detection of DNAzyme cleavage product using molecular beacon

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Chinese Chemical Letters 2012年第10期23卷 1177-1180页

作者： Hong Peng Xie Xiang Xian Meng Hongyan Su Qing Yun Cai Yong Jun Tan Xiao Qin Huang School of Biology State Key Laboratory of Chemo/Biosensing and ChemometricsCollege of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Detection for deoxyribozyme （DNAzyme） cleavage usually needs complex and time-consuming radial labeling, gel electro- phoresis and autoradiography. A new approach was reported for detection DNAzyme cleavage product based on molecular beacon （MB）. Part of the loop of MB was designed to complementary to DNAzyme cleavage product. MB was employed to monitor ligation process of RNA/DNA complex and to convert directly cleavage product information into fluorescence signal. Detection limit of the assay is 0.02 nmol/L. The cleavage product of 8-17 DNAzyme against HCV-RNA was detected perfectly based on this assay. The method is fast, simple and ultrasensitive, which might hold great promise in DNAzyme reaction and DNAzyme gene therapy.

关键词： molecular beacon Ligation DNAzyme cleavage HCV-RNA

A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex molecular beacon for the Detection of Kanamycin

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Chemical Research in Chinese Universities 2018年第4期34卷 541-545页

作者： ZHU Yuqing LI Wei TAN Suzhen CHEN Tianxiao School of Chemistry and Biological Engineering Changsha University of Science and Technology Changsha 410004 P. R. China

A label-free and turn-off fluorescent method for the quantitative detection of kanamycin based on a funtional molecular beacon was developed. The molecular beacon consists of two hairpin structures with a split G-rich oligonucleotide in the middle. The kanamycin＇s aptamer formed the loops portion for recognizing kanamycin, and the G-quadruplex bound by Thioflavin T（ThT） was employed as the reporter. In the absence of target, the molecular beacon folded into double stem-loops and the splited G-rich oligonucleotid came close to form a G-quadruplex. When ThT bound to the G-quadruplex, the fluorescence intensity of the solution increased. Upon the addition of kanamycin, the function between kanamycin and aptamer unfolded the hairpin and disassembled the G-quadraplex structure, resulting in a significant decrease in the fluorescence intensity. A good linear relationship ranging from 0.7 nmol/L to 10 nmol/L was achieved and the limit of detection was 0.37 nmol/L. Besides, it could efficiently recognize kanamycin in real samples.

关键词： molecular beacon Fluorescence Kanamycin G-Quadruplex Thioflavin T

The exploration of quantum dot-molecular beacon based MoS_(2) fluorescence probing for myeloma-related Mirnas detection

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Bioactive Materials 2022年第11期7卷 360-368页

作者： Jing Jing Wang Ying Liu Zhou Ding Le Zhang Caiqin Han Changchun Yan Eric Amador Liqin Yuan Ying Wu Chunyuan Song Ying Liu Wei Chen Jiangsu Key Laboratory of Advanced Laser Materials and Devices School of Physics and Electronic EngineeringJiangsu Normal UniversityXuzhou221116China Jiangsu Key Laboratory for Biosensors Institute of Advanced Materials(IAM)Nanjing University of Posts&TelecommunicationsNanjing210023China Department of Physics The University of Texas at ArlingtonArlingtonTX76019-0059USA Department of General Surgery The Second Xiangya HospitalCentral South UniversityChangShaHu’nan410011China Medical Technology Research Centre Chelmsford CampusAnglia Ruskin UniversityChelmsfordCM11SQUK

Highly sensitive and reliable detection of multiple myeloma remains a major challenge in liquid biopsy. Herein, for the first time, quantum dot-molecular beacon (QD-MB) functionalized MoS_(2) (QD-MB @MoS_(2)) fluorescent probes were designed for the dual detection of multiple myeloma (MM)-related miRNA-155 and miRNA-150. The results indicate that the two probes can effectively detect miRNA-155 and miRNA-150 simultaneously with satisfactory recovery rates, and the limit of detections (LODs) of miRNA-155 and miRNA-150 in human serum are low to 7.19 fM and 5.84 fM, respectively. These results indicate that our method is the most sensitive detection so far reported and that the designed fluorescent probes with signal amplification strategies can achieve highly sensitive detection of MM-related miRNAs for MM diagnosis.

关键词： Multiple myeloma miRNA-155 miRNA-150 Duplex-specific nuclease(DSN) Quantum dot MoS_(2) molecular beacon

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING molecular beacon IMAGING

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Journal of Pharmaceutical Analysis 2006年第2期18卷 167-170页

作者：安瑞芳贺大林薛艳王姝谢丽赵军王新阳杨丽丽 Department of Gynecology and Obstetrics the First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 China Urologic Institute the First Affiliated Hospital of Medical School Xi'an Jiaotong University Xi'an 710061 China Department of Surgery and Winship Cancer Institute Emory University Atlanta

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells (HeLa and SiHa) and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase (SP) assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.

关键词： survivin molecular beacon HeLa SiHa cervical cancer

molecular beacon based Aptazyme for Amplified Fluorescence Detection of Adenosine

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Molecular Beacon based Aptazyme for Amplified Fluorescence D...

molecular beacon-based activatable silver nanoclusters for multiplexed analysis of genes

第八届全国化学生物学学术会议

作者： Yong He Jin Huang Xiaohai Yang Kemin Wang State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan University.Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province

＜正＞Aptazyme is a kind of nucleic acid probes which take advantage of the specific recognition of aptamer and the highly catalysis efficiency of ***,using the elements of 8-17 DNAzyme,adenosine aptamer and molecular... 详细信息

关键词： DNAzyme Aptamer molecular beacon Adenosine

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Molecular beacon-based activatable silver nanoclusters for m...

A versatile sensing platform based on activatable molecular beacon-based silver nanoclusters

中国化学会第30届学术年会

作者： Da-Qian Feng Guoliang Liu Weicheng Zhou Yapeng Shi School of Chemistry and Chemical Engineering Yancheng Institute of Technology

Homogeneous detection of nucleic acids with fluorescent probes attracts the wide interesting due to possessing several inherent advantages such as operation convenience and real-time analysis. Herein, activatable molecular beacon-based silver nanoclusters were applied for the multiplexed detection of Influenza A virus genes. The fluorescence signal is quenched as for the energy transfer from silver nanoclusters to the quencher. Upon the introduction of target gene, the specific combination induces the biological allosteric effectors, preventing the energy transfer, leading to activatable fluorescence intensity. By implementing two different color molecular beacon-based silver nanoclusters, the parallel multiplexed detection of two virus genes using 520, 608 nm fluorescent silver nanoclusters is demonstrated. Furthermore, the developed sensing platform will be used for multiplexed analysis of biologically relevant molecules besides the genes.

关键词： Multiplexed analysis Activatable Silver nanoclusters molecular beacon

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A versatile sensing platform based on activatable molecular ...

Mechanics of molecular beacons for mRNA detecting

中国化学会第30届学术年会

作者： Guoliang Liu Da-Qian Feng Lei Kong Weiwei Diao School of Chemistry and Chemical Engineering Yancheng Institute of Technology

Develop the activatable fluorescence probe has become a research hotspot due to its high sensitivity and superior signal-to-background ratio in the imaging ***,activatable molecular beacon-based silver nanoclusters were synthesized for the development of fluorescent DNA sensors and *** specific combination of targets with the sensing sequence triggers the biological allosteric effectors,which prevent the energy transfer from fluorescence silver nanoclusters to the quencher,resulting in activatable fluorescence *** activatable molecular beacon-based silver nanoclusters are implemented for the analysis of p53 ***,the sensing platform is also applied to determine small molecule ATP or biomacromolecule thrombin.

关键词： Fluorescence-activatable probe Silver nanoclusters molecular beacon Versatile

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Acta Mechanica Sinica 2021年第2期37卷 187-193页

作者： Yulong Gong Xinghua Shi Laboratory of Theoretical and Computational Nanoscience CAS Center for Excellence in NanoscienceNational Center for Nanoscience and TechnologyChinese Academy of Sciences.Beijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Decoding genetic information is crucial for gene therapy and cancer diagnosis,which has attracted growing interest in the field of clinical medicine and life *** this study,we conducted a comprehensive exploration to obtain the detection mechanism of molecular beacons from a mechanics point of *** potential energy function of molecular beacon/target system is established firstly,based on which the profile of molecular beacons is solved by genetic algorithm *** length of stem and the total energy are further calculated when the target is hybridized with loop and *** results show that the hybridization between target and stem is energetically favorable compared with that between target and loop,indicating a new detection *** analyses may cast light on understanding the mechanism of molecular beacons detection,and further help to design novel molecular beacons with high resolution and quantification.

关键词： molecular beacon Detection mechanism Finite deformation model Energy release rate