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Application of molecular marker Assisted Selection in Gene Pyramiding and Selection of New Cultivars

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Journal of Northeast Agricultural University(English Edition) 2011年第1期18卷 79-84页

作者： WU Lan WANG Chao College of Horticulture Northeast Agricultural University Harbin 150000 China

The feasibility of molecule markers＇ application in gene pyramiding has been proved,and obvious progresses in crop breeding have been made till ***,different QTLs or molecular markers linked tightly to yield,quality or resistance may be used for marker assisted *** will be applied widely in crop breeding due to the development of more gene-based markers and efficient quantitative trait locus（QTL） as well as lower cost marking systems.

关键词： molecular marker gene pyramiding breeding

Development of molecular marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

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Journal of Northeast Agricultural University(English Edition) 2012年第4期19卷 30-36页

作者： Li Ning Jiang Jing-bin Li Jing-fu Xu Xiang-yang Cash Crops Research Institute Hubei Academy of Agricultural Sciences Department of Horticulture College of HorticultureNortheast Agricultural University

The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.

关键词： tomato Cf-10 resistant gene Cladosporium fulvum molecular marker

Introduction of T-DNA into Watermelon by Stigma Smeared Method and Its molecular marker Research

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Agricultural Science & Technology 2011年第1期12卷 77-79页

作者： MA Shuang-wu BAO Wen-feng WANG Ji-ming SHANG Jian-li WANG Xiao-jun Zheng Zhou Fruit Research Institute Chinese Academy of Agricultural SciencesZhengzhou 450009 Hefei Fengle Seed Co. Ltd.Hefei 230031

[Objective]The aim was to introduce T-DNA into watermelon for its molecular marker research.[Method]Based on the method of foreign DNA introduced to Arabidopsis thaliana via dipping flowers,the stigma smear was used to transfer T-DNA into watermelon and its molecular marker research was carried out.[Result]The ideal transformed species was ZXG01078 for the highest fruit setting rate and the most deviant *** best concentration of kanamycin for treating watermelon seeds was 500-700 mg/L with differences among the *** best position was spire leaf or young leaf and the best concentration of kanamycin for treating the watermelon leaf was 4 000-8 000 mg/L with no significant difference among *** steadily variation appearing of growing pointless and conjoined twin seedlings indicated that the normal growth had been interfered by foreign DNA in the progeny.[Conclusion]This study had provided basis for the further research on watermelon.

关键词： Watermelon Stigma smeared T-DNA introduced molecular marker

Application of molecular marker Technology in the Study of Forest Tree Species

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Asian Agricultural Research 2022年第12期14卷 32-35页

作者： Yini YAO Fatao HE Yan ZHAO Yuan MENG Yanrui MA Le CHU Guangpeng LIU Jinan Fruit Research Institute All China Federation of Supply and Marketing CooperativesJinan 250014China

Due to its unique advantages, molecular marker technology is widely applied in the research of forest tree species. This paper reviewed the application of molecular marker technology in tree species resource diversity, germplasm identification, genetic map construction, gene mapping and marker-assisted selection (MAS) breeding. In addition, it elaborated the great significance of molecular marker technology to promote the sustainable development of forestry production in China.

关键词： molecular marker Species resource identification Randomly amplified polymorphic DNA(RAPD) Forest tree breeding

Advances in molecular marker Techniques in Pomegranate(Punicagranatum L.)

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Agricultural Science & Technology 2016年第1期17卷 8-11,62页

作者：陆丽娟杨健张水明俞飞飞孙其宝周军永安徽省农业科学院园艺研究所安徽合肥230031 安徽农业大学园艺学院安徽合肥230036

At present, there is a need to develop a universal standard for the classification of pomegranate（Punica granatum L.） species, which facilitates the collection,sorting, classification and application of pomegranate resources. This review introduces recent research progress in pomegranate classification（in particular with molecular markers）, and finally the potential application of molecular markers in future research about pomegranate.

关键词： Pomegranate Classification molecular marker

Genetic Inheritance and molecular marker of Clubroot Resistance Genes in Brassica campestris ssp. chinensis

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Agricultural Science & Technology 2015年第6期16卷 1155-1158页

作者：宋波索欢陈龙正徐海樊小雪张慧袁希汉江苏省农业科学院蔬菜研究所江苏南京210014

Objective] This study was conducted to investigate the genetic inheritance of clubroot resistance in Chinese non-heading cabbage （Brassica campestris ssp. chinensis）. [Method] The clubroot resistance gene was introduced from a Brassica campestris ssp. pekinensis cultivar to non-heading Chinese cabbage, and the inheri-tance and molecular markers of clubroot resistance gene in parental lines, F1, F2 and BC1 of non-heading Chinese cabbage were studied through pathogen inoculation at seedling stage and ISSR-PCR. [Result] Clubroot resistance in non-heading Chi-nese cabbage was control ed by a single dominant gene. ISSR molecular markers with Bulk segregant analysis （BSA） found that primer-873 was linked to resistance gene, named CR-873, and the genetic distance between the marker and the resis-tance gene was 9.72 cM. [Conclusion] The results provide references for the molecular marker assisted breeding of non-heading Chinese cabbage.

关键词： Brassica campestris ssp. chinensis Clubroot resistance molecular marker

Fast tracking alien gene discovery by molecular markers in a late flowering Chinese cabbage-cabbage translocation line‘AT7–4'

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Horticultural Plant Journal 2023年第1期9卷 89-97页

作者： Hui Li Aixia Gu Daling Feng Na Li Rui Yang Xinpei Zhang Shuangxia Luo Umer Karamat Qianyun Wang Shuxin Xuan Xueping Chen Yin Lu Yanhua Wang Shuxing Shen Jianjun Zhao State Key Laboratory of North China Crop Improvement and Regulation Key Laboratory of Vegetable Germplasm Innovation and Utilization of HebeiCollaborative Innovation Center of Vegetable Industry in HebeiBaodingHebei 071000China College of Horticulture Hebei Agricultural UniversityBaodingHebei 071000China College of Life Science Hebei Agricultural UniversityBaodingHebei 071000China

Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding *** our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage ***-specific molecular markers show that these genes were coming from chromosome C01 of *** this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the ***,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week *** on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were *** study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.

关键词： Chinese cabbage Translocation line Alien gene molecular marker Late flowering

Development of two continuous hemocyte cell sublines in the Asian corn borer Ostrinia furnacalis and the identification of molecular markers for hemocytes

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Insect Science 2021年第5期28卷 1382-1398页

作者： Jian Hu Yan Du Meng Meng Yipei Dong Jiewen Peng School of Agriculture Sun Yat-Sen UniversityGuangzhou510275China State key Laboratory of Biocontrol School of Life SciencesSun Yat-Sen UniversityGuangzhou510275China

Granulocytes and plasmatocytes play important roles in clearing foreign objects in insects,but it is difficult to distinguish between them in immune *** on the hemocyte cell line SYSU-OfHe-C established at our lab,two cell sublines,SYSU-OfHe-C Granulocyte(Gr cells)and SYSU-OHe-C Plasmatocyte(PI cells),which possess the morphological characteristics of granulocytes and plasmatocytes,respectively,were *** and PI cells showed different behaviors in immune reactions,such as spreading,phagocytosis and *** cells were easier to spread,but Gr cells tended to undergo aggregation,indicating that they may take different strategies to clear foreign *** results also suggested that granulocytes and plasmatocytes may express some different *** comparing the gene expression in cells from the two sublines,1662 differentially expressed genes were identified,and 13 out of 30 transmembrane proteins highly ex pressed in PI cells(six)or Gr cells(seven)were further screened and confirmed by reverse-transcription polymerase chain reaction(PCR).Finally,three transmembrane genes specifically expressed in Pl cells and two transmembrane genes specifically expressed in Gr cells were screened out based on their expressions in immune reactions by quantitative PCR *** genes may potentially be used as molecular markers to distinguish between granulocy tes and plasmatocytes in Ostrinia fiurnacalis,and further to clarify the functions of immune hemocytes in cellular immune reaction such as encapsulation and so on.

关键词： cell sublines granulocyte hemocyte insect molecular marker plasmatocyte

Application of molecular markers Linking to Cytoplasmic Male Sterile Loci to Assist Maintainer Line Selection and Their Selection Efficiency in Welsh Onion (Allium fistulosum L.)

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Agricultural Sciences in China 2010年第11期9卷 1571-1576页

作者： GAI Shu-peng MENG Xiang-dong College of Life Science Qingdao Agricultural University Qingdao 266109 P.R.China College of Horticulture Shandong Agricultural University Tai ＇an 271018 P.R. China

Cytoplasmic male sterility exists widely in most natural populations of welsh onion （Alliumfistulosum L.）, which makes it possible to breed out many male sterile lines for heterosis utilization. Unfortunately, the breeding of cytoplasmic male sterility in welsh onion has a little progress due to the limitation of its biological characteristic and traditional selection approach. To study the feasibility and the efficiency of utilizing marker assisted selection for male sterile lines in welsh onion, one SCAR marker, SCS13, and one RAPD marker, S2002400, which could distinguish between N and S cytoplasm in several welsh onion cultivars, were identified. The two markers were then confirmed by Southern blotting, and used to screen the N or S cytoplasm of individual plants in seven welsh onion cultivars in this study. Male sterile and fertile plants were evaluated by aceto-carmine dying. The frequency of N-cytoplasmic plants and maintainer genotype was calculated in the seven open populations of welsh onion. The minimum number of plants needed to identify a maintainer was evaluated to be 95% reliable. Results showed that 20 to 80% decrease of crosses and self-crosses for identifying a maintainer genotype could be achieved by the marker-assisted selection compared with traditional selection method. It was proved that the molecular markers could precisely identify cytoplasmic types individually, performed by one generation of cross and two generations of testcrosses and self-crosses. Finally, several maintainer genotype plants were selected with the help of the two markers in the seven cultivars. The screened markers could assist and accelerate sterile and maintainer lines selection with less labor and cost.

关键词： Alliumfistulosum L. CMS (cytoplasmic male sterility) markers-assisted selection molecular marker efficiency