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Enhanced production of laccase by Coriolus hirsutus using molasses distillery wastewater

Frontiers of Environmental Science & Engineering 2013年第2期7卷 200-210页

作者： Wei SUN Meiying XU ChunyuXIA Anhua LI Guoping SUN Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application Guangdong Institute of Microbiology Guangzhou 510070 China Guangdong Open Laboratory of Applied Microbiology Guangzhou 510070 China State Key Laboratory of Applied Microbiology (Ministry--Guangdong Province Jointly Breeding Base) South China Guangzhou 510070 China School of Bioscience and Bioengineering South China University of Technology Guangzhou 510006 China

The effect of physical parameters of cultivation （load volume, temperature, pH, agitation, inoculum size, and incubation period） in the production of laccase by wood-rotting basidiomycete Coriolus hirsutus were stu- died using diluted molasses distillery wastewater （MDW） as a major composition. Using fractional factorial design, our study first identified load volume, agitation, and inoculum size as statistically significant factors. Optimal preferences and mutual interactions of the factors were then determined by the response surface method, which is based on the center composite design. A quadratic model was used to fit the experimental data. The optimized operational parameters for laccase production were determined to be the following： culture temperature of 25~C, pH 4, load volume of 40 mL diluted MDW in 150 mL flask, agitation rate of 183 r-m in-~, inoculation of 11.5% v/v, and cultivation time of 6 d. The experimental validation under these conditions （the maximum laccase production of ***-1 was within the confidence interval） subsequently verified the accuracy of the constructed model. Moreover, the removal of chemical oxygen demand and nitrogen of MDW reached 62.85% and 48.00% respectively, and the decolorization ratio under the optimal condition was 41.85%. The enhanced production of laccase by C. hirsutus is a new recovery strategy for MDW.

关键词： laccase Coriolus hirsutus fractional factorialdesign response surface method molasses distillery wastewater

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