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modified Bfat-1 gene and its biological verification in mice by hydrodynamic tail vein injection

Journal of Integrative Agriculture 2016年第6期15卷 1330-1337页

作者： GAO Xue DU Xin-hua ZHANG lu-pei CHEN Yan GAO Hui-jiang XU Shang-zhong lI Jun-ya Key laboratory for Farm Animal Genetic Resources and Utilization Ministry of AgricultureInstitute of Animal Science Chinese Academy of Agricultural Sciences State Key laboratory of Agro-Biotechnology China Agricultural University

Omega-3 polyunsaturated fatty acids （w-3 PUFAs） are essential components required for normal cellular function and have been shown to have important therapeutic and nutritional benefits in humans. But humans or mammals cannot naturally produce w-3 PUFAs, due to the lack of the co-3 fatty acid desaturase gene （fat-1 gene）. Previously, fat-1 gene has been cloned from Caenorhabditis elegans and transferred into mice, pigs and sheep, but not yet into beef cattle. We attempt to transfer it into beef cattle. The object of this paper is to edit the fat-1 gene from C. elegans to express more efficiently in beef cattle and verify its biological function in mice model. As a result, the fat-1 gene from C. elegans was modified by synonymous codon usage and named it Bfat-l. We have demonstrated that degree of codon bias of brat-1 gene was in- creased in beef cattle. Moreover, Bfat-1 gene could be transiently expressed in mouse liver and muscle, the w-6/co-3 PUFAs ratio of 18 and 20 carbon was decreased significantly in liver （P〈0.05）, and the ratio of 20 carbon decreased significantly in muscle 24 and 72 h after injection （P〈0.05）. This confirms that the Bfat-1 gene modification was successful, and the protein encoded was able to catalyze the conversion of w-6 PUFAs to e0-3 PUFAs.

关键词： beef cattle codon bias modified brat-l hydrodynamic tail vein injection mice

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