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Cytological and transcriptional analysis reveal phosphatidylinositol signaling pathway plays key role in mitotic division of Pyropia yezoensis

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Journal of Oceanology and Limnology 2022年第3期40卷 1148-1159页

作者： Yunke ZHU Xinran WANG Bin SUN Xianghai TANG Yunxiang MAO Key Laboratory of Marine Genetics and Breeding(Ministry of Education) College of Marine Life SciencesOcean University of ChinaQingdao 266003China MOE Key Laboratory of Utilization and Conservation for Tropical Marine Bioresources College of Fisheries and Life ScienceHainan Tropical Ocean UniversitySanya 572022China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

The phosphatidylinositol(PI)signaling system,a central regulator of eukaryotic metabolism,is widely found in eukaryotes for regulating a variety of cell *** of the genes in the PI signaling system were found conserved in Pyropia *** this experiment,wortmannin was used as an inhibitor to inhibit the activity of phosphatidylinositol-3 kinase(PI3K),an important regulator of the PI signaling *** wortmannin treatment,the mitotic division of *** was signifi cantly inhibited in a dose-dependent manner,and the mitotic division percentage was reduced by 68.1%and 91.9%in the 5-and 10-μmol/L groups,*** thalli were treated with wortmannin,the levels of reactive oxygen species(ROS)were signifi cantly ***,the expression level of PI3K was inhibited and the expression levels of downstream genes regulated by PI3K was signifi cantly *** the PI3K-AGC signaling pathway,the expression levels of Serine/threonine protein kinase(AGC)and cyclindependent kinases A(CDKA)were downregulated,while WEE1 kinase gene(WEE1)was *** nicotinamide adenine dinucleotide phosphate(NADPH)oxidase genes were downregulated after wortmannin *** results indicate that the PI signaling system plays an important role in the regulation of cell activity in *** was speculated that the growth and development of *** might be regulated by *** PI3K,which promoted the expression of the AGC gene and further regulates the expression of downstream WEE1 and CDKA genes to advance mitotic division,and also promoted the expression level of NADPH oxidase that regulates ROS homeostasis.

关键词： Pyropia yezoensis phosphatidylinositol signaling system reactive oxygen species mitotic division

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