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中国遗传学会第八次代表大会暨学术讨论会

作者： Zhen Qin Laixiang Xu Mingjing Song Fanhua KongCollege of Life Sciences,Qufu Normal University,Qufu 273165,Shandong,China, Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences,Beijing,100021, China

To test the structure of mitochondrial dna control region and phylogenetic relationships in rodentia,the mitochondrial dna control region of Tscherskia triton,Cricetulus barabensis, Apodemus agrarius and Niviventer niviventer were amplified by polymerase chain reaction(PCR) and directly sequenced,the PCR primers were designed by the consensus sequence of rodentia with Primer *** mtdna control region of rodentia could be separated into three domains,namely, the terminal associated sequence domain,the central conserved sequence domain and the conserved sequence block *** extended terminal associated sequence(ETAS1 and ETAS2),six conserved sequence blocks(CSB-F,CSB-E,CSB-D,CSB-C,CSB-B,CSB-A)in the central conserved sequence domain and three conserved sequence blocks(CSB1,CSB2,CSB3)in the conserved sequence block domain were also *** phylogenetic relationships among rodentia were constructed through UPGMA methods using homo as *** showed that phylogenetic relationships among rodentia made by the mitochondrial dna control region consistent with ncbi.

关键词： rodentia mitochondrial dna control region phylogeny

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Genetic variation of the small yellow croaker(Larimichthys polyactis)inferred from mitochondrial dna provides novel insight into the fluctuation of resources

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Acta Oceanologica Sinica 2022年第11期41卷 88-95页

作者： Jian Zheng Tianxiang Gao Yunrong Yan Na Song Key Laboratory of Mariculture of Ministry of Education Ocean University of ChinaQingdao 266003China Fishery College Zhejiang Ocean UniversityZhoushan 316022China College of Fisheries Guangdong Ocean UniversityZhanjiang 524088China

The small yellow croaker(Larimichthys polyactis)belongs to the family Sciaenidae,which is an offshore warm fish species and widely distributed in the western *** this study,the variation of genetic diversity and genetic differentiation among *** populations was analyzed by mitochondrial dna control region.A total of 110 polymorphic sites were checked,which defined 134 *** level of haplotype diversity(h=0.993±0.002)was detected in the examined *** genetic structure analyse(analysis of molecular variance,Fst)showed there were high gene flow among *** *** result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations,which can be attributed to diverse habitats,wide distribution range and high mutation rate of control *** phylogenetic methods,coalescent analyses(neutrality tests,mismatch distribution analysis,Bayesian skyline analyses)and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence,we inferred that the genetic make-up of extant populations of *** was shaped by Pleistocene environmental impacts on the historical demography of this ***,relatively constant genetic diversity and larger effective population size were detected in recent *** *** result showed that the fishing policy certainly,such as the summer closed fishing,played a role in protecting resources of *** study can offer a wealth of biological novelties which indicates genetic structure of *** population and provides the foundation for resources protection and policy setting.

关键词： Larimichthys polyactis genetic diversity genetic differentiation mitochondrial dna control region

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维普期刊数据库

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Genetic variation and population dispersal of Yangtze voles Microtus fortis calamorum in the Dongting Lake region

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Current Zoology 2012年第2期58卷 211-220页

作者： Zongming GUO Pengfei SONG Cong GUO Zhaobin SONG Yong WANG Bo LI Meiwen ZHANG Jianghong RAN College of Life Sciences Sichuan University Chengdu 610064 Sichuan China Key laboratory agro-ecological processes of Subtropical region Institute of Subtropical Agriculture Chinese Academy of Sciences Changsha 410125 China Yantai Nanshan University Yantai 265713 Shandong China

To understand genetic variation and population dispersal in the Yangtze vole Microtusfortis calamorum distributed in the Dongting Lake region, 144 individuals were collected from six habitat patches. The mitochondrial dna control region was sequenced and 17 haplotypes were observed. Of the six investigated populations, haplotype and nucleotide diversities of those from larger patches were higher than those from smaller patches. Nonparametric correlation analysis showed that patch size had a positive correlation with haplotype diversity （r = 0.943, P 〈 0.01）. A neighbour-joining tree of the 17 haplotypes showed no geo- graphic genetic structure among the six populations. Analysis of isolation by distance showed that genetic differentiation among the six populations was not positively related to geographic distance. Analysis of mismatch distribution indicated that the voles had passed through a population expansion. The pattern of haplotype distribution in the Changsha population suggests that the population was established by a founder effect

关键词： Microtusfortis calamorum mitochondrial dna control region genetic structure Dongting Lake Habitat patch

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A preliminary investigation on genetic diversity of Sousa chinensis in the Pearl River Estuary and Xiamen of Chinese waters

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Journal of Genetics and Genomics 2008年第8期35卷 491-497页

作者： Hongshan Chen Kan Zhai Jialin Chen Yulong Chen Haijun Wen Sufang Chen Yuping Wu State Key Laboratory of Biocontrol Department of Biochemistry School of Life Sciences Sun Yat-Sen University Guangzhou 510275 China National Nature Preserve Administration Bureau on Sousa chinensis of Pearl River Estuary Zhuhai 519000 China The First Affiliated Hospital of Sun Yat-Sen University Guangzhou 510000 China

In this study, the mitochondrial dna （mtdna） control region and the mitochondrial cytochrome b gene of stranded Indo-Pacific humpback dolphin （Sousa chinensis） samples from the Pearl River Estuary and Xiamen waters were sequenced and analyzed. The result of mtdna control region revealed 34 variable sites and four unique haplotypes （named as A, B, C and D） identified among the total samples from these two water areas, and the most common haplotype （A） was shared by 75% of the dolphins sampled from the two water areas. The haplotypic diversity （h） was 0.455 and the nucleodde diversity （π） was 0.0088. The phylogenetic analysis showed that the haplotype A, C, and D were closely related, but the haplotype B （unique for XM01 from Xiamen） was far from the other three. By scanning cytochrome b fragments, two haplotypes （A and B） were identified in these two water areas, and the most common haplotype （A） was shared by 91.67% individuals, while XM01 from Xiamen as the only exception. The data suggest that there is a possibility of gene exchange between the two populations in the Pearl River Estuary and Xiamen waters, and there possibly exists a unique maternal lineage in Xiamen waters.

关键词： Sousa chinensis mitochondrial dna control region cytochrome b genetic diversity

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