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mirnas targeting CYP6ER1 and CarE1 are involved in nitenpyram resistance in Nilaparvata lugens

Insect Science 2022年第1期29卷 177-187页

作者： Kaikai Mao Ruoheng Jin Zhijie Ren Junjie Zhang Zhao Li Shun He Kangsheng Ma Hu Wan Jianhong Li Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

The evolution of nitenpyram resistance has been confirmed to be related to overexpression of two key metabolic enzyme genes, CYP6ER1 and CarE1, in Nilaparvata lugens, a highly destructive rice pest that causes substantial economic losses and has developed insecticide resistance. As microRNAs (mirnas) are important post-transcriptional regulators of gene expression, whether they are involved in nitenpyram resistance is poorly understood in N. lugens. In this study, knockdown of key genes in the miRNA biogenesis pathway (Dicer1, Drosha, and Argonaute1) changed CYP6ER1 and CarE1 abundance, which confirmed the importance of mirnas in nitenpyram resistance. Furthermore, global screening of mirnas associated with nitenpyram resistance in N. lugens was performed, and a total of 42 known and 178 novel mirnas were identified;of these, 57 were differentially expressed between the susceptible and resistant strains, and two (novel_85 and novel_191) were predicted to target CYP6ER1 and CarE1, respectively. Luciferase reporter assays demonstrated that novel_85 and novel_191 bind to the CYP6ER1 and CarE1 coding regions, respectively, and downregulate their expression. Moreover, modulating novel_85 and novel_191 expression by injection of miRNA inhibitors and mimics significantly altered N. lugens nitenpyram susceptibility. This is the first study to systematically screen and identify mirnas associated with N. lugens nitenpyram resistance, and provides important information that can be used to develop new miRNA-based targets in insecticide resistance management.

关键词： CarE1 CYP6ER1 mirnas Nilaparvata lugens nitenpyram resistance

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Microfluidic Device for Simple Diagnosis of Plant Growth Condition by Detecting mirnas from Filtered Plant Extracts

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Plant Phenomics 2024年第2期6卷 364-376页

作者： Yaichi Kawakatsu Ryo Okada Mitsuo Hara Hiroki Tsutsui Naoki Yanagisawa Tetsuya Higashiyama Akihide Arima Yoshinobu Baba Ken-ichi Kurotani Michitaka Notaguchi Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Department of Molecular and Macromolecular Chemistry Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoyaAichi 464-8603Japan Graduate School of Science Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Institute of Transformative Bio-Molecules Nagoya UniversityNagoya 464-8601Japan Department of Biological Sciences Graduate School of ScienceThe University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan Institute of Nano-Life-Systems Institutes of Innovation for Future SocietyNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Department of Biomolecular Engineering Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Institute of Quantum Life Science National Institutes for Quantum Science and Technology(QST)Anagawa 4-9-1Inage-kuChiba 263-8555Japan Department of Botany Graduate School of ScienceKyoto UniversityKitashirakawa Oiwake-choSakyo-kuKyoto 606-8502Japan

Plants are exposed to a variety of environmental stress,and starvation of inorganic phosphorus can be a major constraint in crop *** plants,in response to phosphate deficiency in soil,miR399,a type of microRNA(miRNA),is *** detecting miR399,the early diagnosis of phosphorus deficiency stress in plants can be ***,general miRNA detection methods require complicated experimental ***,simple and rapid miRNA detection methods are required for early plant nutritional diagnosis.

关键词： mirnas plant device simple growth from condition detecting diagnosis extracts

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mirnas modulate the drug response of tumor cells

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Science China(Life Sciences) 2009年第9期52卷 797-801页

作者： WU XueMei1,3 & XIAO HuaSheng1,2 1 Center of Functional Genomics,Key Laboratory of System Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200031,China 2 National Engineering Center for Biochip at Shanghai,Shanghai 201203,China 3 Graduate University of Chinese Academy of Sciences,Shanghai 200031,China Center of Functional Genomics Key Laboratory of System Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China Graduate University of Chinese Academy of Sciences Shanghai China National Engineering Center for Biochip at Shanghai Shanghai China

Chemotherapy is one of the major treatments of malignant carcinomas. However,its efficiency is affected by both intrinsic and acquired resistance to anticancer drugs. The cellular mechanisms of drug resistance include the overexpression of energy-dependent transporters that eject anticancer drugs from cells such as p-glycoprotein and multidrug resistance related protein (MRP),the mutation of drug targets,the activation of DNA repair pathways,the defects in cellular death pathways and so on. The genetic and epigenetic changes of these genes can lead to cancer drug resistance. Among these mechanisms,microRNAs (mirnas) which are critical and essential for many important processes such as development,differentiation,and even carcinogenesis have been reported to regulate the chemosen-sitivity of tumor cells. In this paper we briefly review the relationship between miRNA and cancer drug resistance.

关键词： cancer drug resistance mirnas

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Differentially expressed mirnas in anthers may contribute to the fertility of a novel Brassica napus genic male sterile line CN12A

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Journal of Integrative Agriculture 2020年第7期19卷 1731-1742页

作者： DONG Yun WANG Yi JIN Feng-wei XING Lijuan FANG Yan ZHANG Zheng-ying ZOU Jun-jie WANG Lei XU Miao-yun Crop Research Institute Gansu Academy of Agricultural SciencesLanzhou 730070P.R.China National Key Facility for Crop Gene Resources and Genetic ImprovementBiotechnology Research Institute Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Gansu Provincial Key Lab of Aridland Crop Sciences/Gansu Agricultural University Lanzhou 730070.P.R.China

In Brassica napus L.(rapeseed),complete genic male sterility(GMS)plays an important role in the utilization of *** microRNAs(mirnas)play essential regulatory roles during bud development,knowledge of how GMS is regulated by mirnas in rapeseed is rather *** this study,we obtained a novel recessive GMS system,*** sterile line CN12A has defects in tapetal diferentiation and *** sequencing was employed to examine the expression of mirnas in the buds of CN12A and the fertile line *** identifed 85 known mirnas and 120 novel mirnas that were expressed during rapeseed anther *** comparing the expression levels of mirnas between CN12A and CN12B,19 and 18 known mirnas were found to be difrentially expressed in 0.5--1.0 mm buds and in 2.5--3.0 mm buds,*** these,the expression levels of 14 mirnas were higher and the levels of 23 mirnas were lower in CN12A compared with *** predicted target genes of these iferentially expressed mirnas encode protein kinases,F-box domain-containing proteins,MADS-box family proteins,SBP-box gene family members,HD ZIP proteins,floral homeotic protein APETAL A 2(AP2)。and nuclear factor Y,subunit *** targets have previously been reported to be involved in pollen development and male sterility,suggesting that mirnas might act as regulators of GMS in rapeseed ***,RT-qPCR data suggest that one of the dffrentially expressed mirnas,bna-miR159,plays a role in tapetal differentiation by regulating the expression of transcription factor BnMYB101 and participates in tapetal degradation and infuences callose degradation by manipulating the expression of *** findings contibute to our understanding of the roles of mirnas during anther development and the occurrence of GMS in rapeseed.

关键词： Brassica napus L. genic male sterility mirnas targets molecular regulation network

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Impact of tiny mirnas on cancers

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World Journal of Gastroenterology 2007年第4期13卷 497-502页

作者： Wei Liu Sheng-Yong Mao Wei-Yun Zhu Laboratory of Gastrointestinal Microbiology College of Animal Science and Technology Nanjing Agricultural University Nanjing 210095 China

mirnas are a class of small, ∽22nt, non-coding RNAs that negatively regulate gene expression at the posttranscriptional level. They play profound and pervasive roles in manipulating gene expression involved in cell development, proliferation and apoptosis in various eukaryotes, which, in theory, could provide an access to many human diseases in theory. Recent evidence demonstrates that aberrant miRNA expression is a hallmark of tumor development, revealing that miRNA genes could function as potential oncogenes and repressors in the human body. mirnas can affect tumorigenesis mainly by interrupting the cell cycle at the cellular level and by interacting with signaling, oncogenes and with the response to environmental factors at the molecular level. The established miRNA expression signature could be a potent tool to diagnose and treat human cancers in the future.

关键词： mirnas Cancers Oncogenes and repressors Tumorigenesis miRNA expression signature

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Circulating mirnas as biomarkers for severe acute pancreatitis associated with acute lung injury

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World Journal of Gastroenterology 2017年第41期23卷 7440-7449页

作者： Xiao-Guang Lu Xin Kang Li-Bin Zhan Li-Min Kang Zhi-Wei Fan Li-Zhi Bai Depart-ment of Emergency Zhongshan Hospital Dalian University College of Basic Medicine Nanjing University of Chinese Medicine Department of Hepatobiliary and Pancreatic Surgery Puer People's Hospital

AIM To identify circulating micro(mi)RNAs as biological markers for prediction of severe acute pancreatitis(SAP) with acute lung injury(ALI).METHODS Twenty-four serum samples were respectively collected and classified as SAP associated with ALI and SAP without ALI, and the mi RNA expression profiles were determined by microarray analysis. These mi RNAs were validated by quantitative reverse transcriptionpolymerase chain reaction, and their putative targets were predicted by the online software Target Scan, mi Randa and Pic Tar database. Gene ontology(GO) and Kyoto encyclopedia of genes and genomes(commonly known as KEGG) were used to predict their possible functions and pathways *** We investigated 287 mi RNAs based on microarray data analysis. Twelve mi RNAs were differentially expressed in the patients with SAP with ALI and those with SAP without ALI. Hsa-mi R-1260 b, 762, 22-3 p, 23 b and 23 a were differently up-regulated and hsa-mi R-550 a*, 324-5 p, 484, 331-3 p, 140-3 p, 342-3 p and 150 were differently down-regulated in patients with SAP with ALI compared to those with SAP without ALI. In addition, 85 putative target genes of the significantly dysregulated mi RNAs were found by Target Scan, mi Randa and Pic Tar. Finally, GO and pathway network analysis showed that they were mainly enriched in signal transduction, metabolic processes, cytoplasm and cell *** This is the first study to identify 12 circulating mi RNAs in patients with SAP with ALI, which may be biomarkers for prediction of ALI after SAP.

关键词： mirnas Severe acute pancreatitis Acute lung injury Biomarker Microarray analysis

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Physical activity responsive mirnas-Potential mediators of training responses in human skeletal muscle?

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Journal of Sport and Health Science 2013年第2期2卷 101-103页

作者： Reeta Kangas Eija Pllnen Gerontology Research Center Department of Health SciencesUniversity of Jyvaskyla

*** basics of miRNA-mediated regulation The plasticity of skeletal muscle is of utmost importance for responding to and coping with environmental demands that emerge from changes in physical activity patterns,nourishment, hormonal status,and *** is well known,a sedentary lifestyle,aging,immobilization,and chronic diseases are associated with reduced muscle mass and function,while regular exercise improves muscle function and reduces the rate of decrement throughout ***,we do not have a complete understanding of the molecular factors controlling skeletal muscle adaptation to exercise *** identified microRNA molecules(mirnas) have rapidly

关键词： mirnas 肌肉人体骨骼动响应 microRNA 体力反应训练

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Identification of exosomal mirnas as diagnostic biomarkers for cholangiocarcinoma and gallbladder carcinoma

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1638-1640页

作者： Xin-ying Xue Yu-xia Liu Chen Wang Xin-jin Gu Zhi-qiang Xue Xue-lei Zang Xi-dong Ma Hui Deng Rong Liu Lei Pan San-hong Liu Department of Respiratory and Critical Care Medicine Beijing Shijitan HospitalCapital Medical UniversityBeijingChina Department of Respiratory and Critical Care Medicine General Hospital of PLABeijingChina Department of Respiratory and Critical Care Medicine Hospital of Weifang Medical UniversityShandongChina Department of Scientific Research Peking Union Medical College HospitalBeijingChina Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech UniversityShanghaiChina Department of Hepatobiliary and Pancreatic Oncology Surgery General Hospital of PLABeijingChina Department of Thoracic Surgery General Hospital of PLABeijingChina Department of Laboratory General Hospital of PLABeijingChina

Dear Editor,Extracellular vesicles,especially exosomes,have emerged as promising diagnostic sources for cancers due to their easy and quick accessibility.1,2 Additionally,it has been demonstrated that circulating mirnas serve as promising biomarkers for the diagnosis of multiple diseases.3,4 Cholangiocarcinoma(CCA)and gallbladder carcinoma(GBC)are two malignant biliary tract *** has been estimated that patients with unresectable GBC have a 5-year survival of only less than 5%,while CCA patients suffer from an overall 5-year survival of~10%.5 Hence,circulating diagnostic biomarkers would benefit the diagnosis and treatment of GBC and CCA *** sequenced exosomal small RNA from five CCA patients and four GBC patients before and after surgery and enrolled 40 healthy individuals,45 more CCA patients and 24 more GBC patients to validate the sequencing results to identify diagnostic biomarkers for GBC and CCA.

关键词： diagnosis gallbladder mirnas

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Construction of miRNA-mRNA network reveals crucial mirnas and genes in acute myocardial infarction

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The Journal of Biomedical Research 2021年第6期35卷 425-435页

作者： Kai Wang Zhongming Li Wenjie Ma Yan Sun Xianling Liu Lijun Qian Jian Hong Dasheng Lu Jing Zhang Di Xu Department of Cardiology the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Geriatrics the First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Cardiology the Second Affiliated Hospital of Wannan Medical CollegeWuhuAnhui 241000China.

Acute myocardial infarction(AMI)is a severe cardiovascular *** study aimed to identify crucial microRNAs(mirnas)and mRNAs in AMI by establishing a miRNA-mRNA *** microarray datasets GSE31568,GSE148153,and GSE66360 were downloaded from the Gene Expression Omnibus(GEO)*** identified differentially expressed mirnas(DE-mirnas)and mRNAs(DE-mRNAs)in AMI samples compared with normal control *** consistently changing mirnas in both GSE31568 and GSE148153 datasets were selected as candidate *** interactions between the candidate DE-mirnas and DE-mRNAs were analyzed,and a miRNA-mRNA network and a protein-protein interaction network were constructed,along with functional enrichment and pathway analyses.A total of 209 DE-mirnas in the GSE31568 dataset,857 DE-mirnas in the GSE148153 dataset,and 351 DE-mRNAs in the GSE66360 dataset were *** candidate DE-mirnas were selected from both the GSE31568 and GSE148153 ***,miR-646,miR-127-5p,miR-509-5p,miR-509-3-5p,and miR-767-5p were shown to have a higher degree in the miRNA-mRNA ***-1 as well as FOS was a hub gene in the miRNA-mRNA network and the protein-protein interaction(PPI)network,***1A was important in both miRNA-mRNA network and PPI *** established a miRNA-mRNA network in AMI and identified five mirnas and three genes,which might be used as biomarkers and potential therapeutic targets for patients with AMI.

关键词： acute myocardial infarction mirnas mRNAs miRNA-mRNA regulatory network

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The mirnas of Herpes Simplex Virus(HSV)

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Virologica Sinica 2012年第6期27卷 333-338页

作者： Le Sun Qihan Li Department of Viral Immunology Institute of Medical BiologyChinese Academy of Medicine SciencePekingUnion Medical CollegeKunming 650118China

Herpes simplex virus (HSV) is a group of common human pathogens with two serotypes HSV-1 and *** prevalence of HSV is *** primarily infects humans through epithelial cells,when it introduces a latent infection into the nervous *** viral latency,only a region known as the latency-associated transcript (LAT) is *** discovery of HSV mirnas helps to draw a larger picture of the infection and pathogenesis of the *** review summarizes mirnas found in HSV-1 and HSV-2 so *** functional studies of mirnas in HSV to date indicate that they play a stage-specific role coordinated with viral proteins to maintain the virus life cycle.

关键词： Herpes simplex virus (HSV) mirnas Latency-associated transcript (LAT)

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