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Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting β-catenin

Asian Pacific Journal of Tropical Medicine 2015年第11期8卷 903-908页

作者： Li-Yan Wang Bin Li Huan-Huan Jiang Li-Wei Zhuang Yong Liu Digestive System Department Affiliated Hospital of Guilin Medical College Digestive System Department the Fourth Hospital Affiliated to Harbin Medical University

Objective:To investigate the expression and the regulation effect of cell growth of microRNA-577 in hepatocellular carcinoma(HCC).Methods:qRT-PCR was applied to detect the relative expression of miR-577 in 70 paired HCC and matched tumor adjacent tissues collecting from resection between March 2011 and March *** chi-square test was used to analyze the relationship between the miR-577 expression and clinical *** miR-577 mimics were transfected into HepG2 cells:cell cycles were detected by flow cytometry,cell proliferation was measured by MTT assay and BrdU incorporation assay,and cell apoptosis was determined by flow cytometry and Caspase 3/7 activity *** expressions ofβ-catenin were measured by *** correlation analysis was used to analyze the relationship between miR-577 and ***-PCR and westernblot were used to detect the expression of p-catenin in transfected HepG2 ***:The relative expressions of miR-577 was significantly lower in HCC tissues compared to the matched normal tumor-adjacent tissues(P577 was significantly associated with large tumor size(≥5 cm,P577 mimics could inhibit repress cell proliferation,enhance cell apoptosis and block the cell cycles in G_1/G_1 phase(P577 in HCC group had a significant negative correlation relationship with the expression of downstream target of β-catenin(P577 is related to the malignant clinicopathological features in HCC tissues,and miR-577 may suppress HCC growth through down-regulating p-catenin.

关键词： mircorna-577 Hepatocellular carcinoma β-catenin Pr

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