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Biosorption of Cd (II), Cr (VI) &Pb (II) from Aqueous Solution Using <i>mirabilis jalapa</i>as Adsorbent

Journal of Encapsulation and Adsorption Sciences 2015年第2期5卷 93-104页

作者： Shaik Abdul Shahanaz Begum Yadamari Tharakeswar Yakkala Kalyan Gurijala Ramakrishna Naidu Department of Environmental Sciences Sri Venkateswara University Tirupati India

mirabilis jalapa (chandrakantha) leaves were used as a biosorbent for the removal of toxic metals ions cadmium (II), chromium (VI) and lead (II) from water. The physiochemical properties of the developed biosorbent were studied using FTIR. The efficiency of this developed biosorbent was tested using batch experiments under controlled conditions such as a function of pH, contact time, initial metal concentration and the optimization amount of biosorbent. The residual metallic ion concentrations of cadmium (II), chromium (VI) and lead (II) were determined using AAS. The biosorption capacity of mirabilis jalapa was found to be pH dependant and the percentage removal of cadmium (II), chromium (VI) and lead (II) was increased with increasing adsorbate solution pH and a maximum value was reached at an equilibrium pH = 6 for cadmium (II), pH = 5.5 for chromium (VI) and pH 4.5 for lead (II) respectively. The equilibrium time required for the saturation loading of cadmium (II), chromium (VI) and lead (II) in the biosorbent was found to be about 120 min. The obtained equilibrium biosorption data was fitted to the linear forms of Freundlich isotherms. The results proved the efficiency of mirabilis jalapa leaves powder as biosorbent for the removal of metal ions and it can be used for the development of an efficient, clean and novel technology for waste water treatment.

关键词： mirabilis jalapa Biosorption Toxic Metal Ions Isotherm Models

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Antimicrobial Resistance and Genotype Analysis of Extended-Spectrum-β-Lactamase-Producing Proteus mirabilis

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Open Journal of Clinical Diagnostics 2014年第1期4卷 57-62页

作者： Ying Huang Yuanhong Xu Zhongxin Wang Xianghong Lin Clinical Laboratory the First Hospital Affiliated to Anhui Medical University Hefei China

To analyse the genotypes of clinical isolates of Extended-Spectrum-β-Lactamase-Producing (ESBL-producing) Proteus mirabilis (P. mirabilis) and the mechanisms of antimicrobial resistance, to guide reasonable use of antibiotics and to avoid nosocomial outbreak infections by ESBL-producing P. mirabilis. 125 clinical isolates of P. mirabilis were collected from the Drug-Resistant Bacteria Surveillance Center of Anhui Province (from Jan 2009 to May 2010). Searching for the genotypes of ESBLs was perfomed by PCR amplification and DNA sequencing, and performed conjugation test simultaneously. Among ESBL-producing strains, CTX-M was the major genotype (3 CTX-M-13 and 1 CTX-M-3). TEM-1b spectrum β-lactamase was also prevalence in P. mirabilis. The diversity of β-lactamases in P. mirabilis and the emergency of multi-drug-resistance clinical strains will present serious threat to clinical therapy and even will lead to outbreak of nosocomial infections. Our study emphasizes the need for enhanced supervision of ESBL-producing P. mirabilis. Timely and reasonable drug-resistance data are indispensable to clinical therapy.

关键词： Genotype Extended-Spectrum-β-Lactamase Antimicrobial Resistance Proteus mirabilis

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Extracellular Biosynthesis of AgNPs by the Bacterium <i>Proteus mirabilis</i>and Its Toxic Effect on Some Aspects of Animal Physiology

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Advances in Nanoparticles 2014年第3期3卷 83-91页

作者： Mohammad S. Al-Harbi Bahig A. El-Deeb Nasser Mostafa Sayed A. M. Amer Department of Biology Faculty of Science Taif University Taif Saudi Arabia Department of Biology Faculty of Science Taif University Taif Saudi Arabia Department of Botany Faculty of Science Sohag University Sohag Egypt Department of Biology Faculty of Science Taif University Taif Saudi Arabia Department of Zoology Faculty of Science Cairo University Giza Egypt Department of Chemistry Faculty of Science Taif University Taif Saudi Arabia Department of Chemistry Faculty of Science Suez Canal University Ismailia Egypt

The development of a reliable green chemistry process for the biogenic synthesis of nanomaterials is an important aspect of current nanotechnology research. Silver nanoparticles (AgNPs) have been used as antimicrobial and disinfectant agents. However, there is limited information about its toxicity. Therefore, this study focused on the biosynthesis of AgNPs by the bacterium Proteus mirabilis and on determining its preliminary toxic effect on some aspects of animal physiology. A green method for the synthesis of AgNPs using culture supernatant of Proteus mirabilis has been developed in this study and the synthesized AgNPs were characterized by several techniques. The AgNPs showed a maximum absorbance at 445 nm on ultraviolet-visible spectra. The presence of proteins was identified by Fourier transform-infrared spectroscopy. The reduction of Ag+ to elemental silver was characterized by X-ray spectroscopy analysis. The transmission electron micrograph revealed the formation of polydispersed nanoparticles of 5 - 45 nm. The AgNPs were evaluated for their toxic effect on pregnant female albino rat. The result showed that liver enzymes (AST and ALP) were decreased significantly in the group treated with AgNPs. Mean corpuscular hemoglobin concentration also showed significant increase.

关键词： AgNPs P. mirabilis TEM X-Ray Liver Enzymes Blood Parameters

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Investigation of the Potential Association between Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR) and Antibiotic Resistance Pattern of Bacterial Strains Isolated from Medical Waste and Environmental Water

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Open Journal of Medical Microbiology 2018年第2期8卷 13-25页

作者： Md.Shahariar Alam Sajib Chakraborty Taibur Rahman Md.Ismail Hosen Anik Paul A.K.M.Mahbub Hasan M.Anwar Hossain Department of Biochemistry and Molecular Biology University of DhakaDhakaBangladesh

Enterobacteriaceae are important human pathogens that cause many food and waterborne illness. Rapid emergence of multi-drug resistant bacteria in Bangladesh has become a serious problem. Phage-host interaction is now considered as the major driving force for the conversion of non-pathogenic bacteria to pathogenic ones. Evolution of highly pathogenic and antibiotic resistant bacteria largely depends upon the horizontal gene transfer by means of plasmid, megaplasmid and bacteriophages. Conversely, bacteria may acquire a novel defence mechanism called CRISPR (Clustered regularly interspersed short palindromic repeats) that can restrict horizontal transfer of plasmids and bacteriophages to limit the spread of antibiotic resistance genes among bacterial species. In this study, twenty bacterial strains were isolated from water of different medical waste and Buriganga river. Therefore, CRISPR locus was investigated following various biochemical and molecular analysis of those bacterial isolates. Identification of the bacterial isolates was conducted by Polymerase Chain Reaction (PCR) based assay of 16S rDNA extracted from those isolated strains. Results indicated that most strains were identified as Proteus mirabilis and Citrobacter freundii which mainly cause septicemia, and pneumonia in human. Thereafter, antibiogram of these strains was performed by using 11 different antibiotic discs where bacterial isolates from medical drainage system showed more resistant to antibiotics than the river water. In this study, CRISPR locus was also investigated within the genome of the isolated bacterial stains but unexpectedly, we did not find any CRISPR locus in their genome. In conclusion, we confirm that multi-drug resistant bacterial strains are devoid of CRISPR locus suggesting a possible negative association between CRISPR locus and antibiotic resistance. Further studies to pinpoint are required to elucidate the underlying mechanism of the association between CRISPR and antibioti

关键词： Enterobacteriaceae Antibiotic Resistance Proteus mirabilis Citrobacter freundii CRISPR-Cas

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