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Characterization of interspecific hybrids and backcross progenies from a cross between Oryza minuta and Oryza sativa

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Science China(Life Sciences) 2009年第12期52卷 1148-1155页

作者： GUO SiBin, QIN FaLan, ZHANG DuanPin & LIN XingHua· National Key Laboratory of Crop Genetic Improvement, National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan 430070, China National Key Laboratory of Crop Genetic Improvement National Center of Crop Molecular Breeding Huazhong Agricultural University Wuhan 430070 China

Oryza minuta, a tetraploid wild relative of cultivated rice, is an important source for the genetic improvement. Interspecific hybrids were obtained from the cross of O. sativa L. (IR24) and O. minuta (Acc. No. 101133) with 5.58% crossability, which ranged from 0.11% to 1.62% in the backcross generations. The chromosome numbers of the backcross progenies were 24 to 48. Seven yield-related traits of the parents, hybrid F1, and backcross progenies were evaluated. Simple sequence repeat markers analysis showed that the polymorphism ratio of SSR bands between IR24 and Acc. No. 101133 was 93.2%. The average donor segment number, length, donor genome size, and percentage of donor genome of 92 BC3F1 plants (2n=24) were 24.1, 17.8 cM, 438.4 cM and 26.2%, respectively. They were complex variation and uneven among the chromosomes. These introgression lines could be used to identify the favorable genes of O. minuta and provide a new platform for the genetic improvement of cultivated rice.

关键词： Oryza sativa L. Oryza minuta interspecific hybridization backcross progeny introgression line

Anti-oxidative and anti-inflammatory effects of Tagetes minuta essential oil in activated macrophages

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Asian Pacific Journal of Tropical Biomedicine 2014年第3期4卷 219-227页

作者： Parastoo Karimian Gholamreza Kavoosi Zahra Amirghofran Biotechnology Institute Shiraz UniversityShiraz71441-65186Iran Department of Immunology Autoimmune Disease Research Center and Medicinal and Natural Products Chemistry Research CenterShiraz Universityof Medical SciencesShirazIran

Objective:To investigate antioxidant and anti-inflammatory effects of Tagetes minuta(***)essential ***:In the present study *** essential oil was obtained from leaves of *** via hydro-distillation and then was analyzed by gas chromatography-mass *** antioxidant capacity of *** essential oil was examined by measuring reactive oxygen,reactive nitrogen species and hydrogen peroxide *** anti-inflammatory activity of *** essential oil was determined through measuring NADH oxidase,inducible nitric oxide synthase and TNF-αmRNA expression in lipopolysacharide-stimulated murine macrophages using realtime ***:Gas chromatography-mass spectrometry analysis indicated that the main components in the *** essential oil were dihydrotagetone(33.86%),E-ocimene(19.92%).tagetone(16.15%),cis-β-ocimene(7.94%),Z-ocimene(5.27%).limonene(3.1%)and epoxyocimene(2.03%).The *** essential oil had the ability to scavenge all reactive oxygen/reactive nitrogen species radicals with IC_(50)12-15μg/mL,which indicated a potent radical scavenging *** addition,*** essential oil significantly reduced NADH oxidase,inducible nitric oxide synthaseand TNF-αmRNA expression in the cells at concentrations of 50μg/mL,indicating a capacity of this product to potentially modulate/diminish immune ***:*** essential oil has radical scavenging and anti-inflammatory activities and could potentially be used as a safe effective source of natural anti-oxidants in therapy against oxidative damage and stress associated with some inflammatory conditions.

关键词： Tagetes minuta Essential oil Macrophages Anti-inflammatory Antioxidant

Responses of <i>Marsilea minuta</i>L. to Cadmium Stress and Assessment of Some Oxidative Biomarkers

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American Journal of Plant Sciences 2014年第10期5卷 1467-1476页

作者： Kingsuk Das Chiranjib Mandal Nirmalya Ghosh Narottam Dey Malay Kumar Adak Department of Biotechnology Visva Bharati Santiniketan India Plant Physiology and Plant Molecular Biology Research Unit Department of Botany University of Kalyani Kalyani India

In a hydroponic based experiment, the Cd toxicity is monitored with some cellular responses of Marsilea plant. Initially, plants were grown under varying concentrations (0, 50, 100 and 200 μM of Cd) of cadmium (Cd) with supplementation of 2 mM spermidine (Spd). The oxidative stress developed by Cd overaccumulation was measured with fall in Relative Growth Rate (RGR) by 27.11% to 59.83% growth reduction over control under varying Cd treatments. The retrieval of RGR was recovered by 1.59 folds as compared to the highest concentration of Cd (200 μM) when plants were fed with Spd. A concomitant degradation of chlorophyll was recorded in dose-dependant manner, however, the retrieval was not much pronounced with Spd. On the contrary, the non-oxidant thiol had borne more clarity with ongoing Cd concentration and appeared to be 40.51% increase maximally for GSH: GSSG at the highest concentration of Cd. Spd has minimized the ratio by 27.4%. The recovery of osmotic turgidity was indexed with a sharp rise in glycine betaine by 3.86 folds maximum at the highest concentration of Cd over control which declined by 30.9% with Spd. Another cellular response of treated plants was more evident from their isozymic profiles with regard to superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX). The intensity of protein expression was significantly variable but not in band numbers as evident from Cd treated plants. In vitro enzyme assay of catalase showed as declining trend within the limit of 33.13% to 43.22% which was reported by 1.45 folds when Spd was applied. Therefore, from the present study, the cellular responses of Marsilea plant which showed compatibility for their expression with Cd toxicity could be hypothesized as a case of bioindication.

关键词： Cadmium Toxicity Marsilea minuta Bioindicators

利用昆虫携带的花粉初探西藏入侵植物印加孔雀草在当地传粉网络中的地位

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生物多样性 2019年第3期27卷 306-313页

作者：土艳丽王力平王喜龙王林林段元文西藏自治区高原生物研究所拉萨850001 中国科学院昆明植物研究所昆明650201 中国科学院大学北京100049

入侵植物在新生境中成功定殖后,通过利用当地传粉昆虫促进繁殖可以更好地保证种群的扩张,但是入侵植物在当地传粉网络中的角色和地位仍不是很清楚。本文利用西藏近年发现的入侵植物印加孔雀草(Tagetes minuta),分析其访花昆虫所携带的... 详细信息

入侵植物在新生境中成功定殖后,通过利用当地传粉昆虫促进繁殖可以更好地保证种群的扩张,但是入侵植物在当地传粉网络中的角色和地位仍不是很清楚。本文利用西藏近年发现的入侵植物印加孔雀草(Tagetes minuta),分析其访花昆虫所携带的植物花粉种类,构建了植物花粉–传粉者网络,探讨印加孔雀草快速入侵和扩张的可能机制。结果表明印加孔雀草为泛化传粉系统,共有13种昆虫访花,其中12种携带有印加孔雀草花粉,所有花粉中印加孔雀草花粉数量占比为89.89%。12种印加孔雀草传粉昆虫中, 4种泛化传粉昆虫(1种蜂、2种食蚜蝇和1种蝇)是其主要传粉昆虫。本研究揭示印加孔雀草在较短时间内已经成功利用多种当地泛化传粉昆虫为其授粉,已顺利融入当地的传粉网络,今后需要更加重视对印加孔雀草的防控。

关键词：青藏高原 Tagetes minuta 入侵植物传粉网络花粉

MORPHOLOGICAL COMPARISON OF THE FEEDING APPARATUS OF THREE CO-OCCURRING SPECIES OF PONYFISHES (LEIOGNATHIDAE)

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Chinese Journal of Oceanology and Limnology 1997年第3期15卷 224-226页

作者：刘静

Ponyfishes of the family Leiognathidae frequently show overlapping distribution pattems in the Indo-Pacific and South China Sea. The mouth morphology of three species of ponyfishes which feed at different lerels in the water column were compared. The species could be distinguished primarily by the mouth meropology. Gazza minuta (Bloch)feeds mainly on bethos, has the least protruding jaws; Secutor ruconius (Hamilton-Buchanan) feeds mainly on plankton, has the most protruding jaws, Leiognathus rivuatus (Temmink et Schlegel) has an intermediate deggree of jaw protrusion. This study showed that closely related species with overlapping distributions frequently exhibit morphologlcal, phpsiological or ecological differences related to the search for food, the intake of food or locomotion mode.

关键词： Gazza minuta Leiognathus rivulatus Secutor ruconius feeding apparatus jaw protrusion

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一株分离自胡杨树杆液的内生酵母菌初步鉴定

新疆农业科学 2012年第8期49卷 1483-1488页

作者：祖母拉提.阿布都热依木程刚布阿依夏姆.阿木提努力曼姑.司马义热孜亚.艾肯木合塔尔.阿布都克里木艾尔肯.热合曼新疆大学生命科学与技术学院乌鲁木齐830046

【目的】依据形态学、生理生化和26S rDNA D1/D2区序列特征,确定一株胡杨内生酵母菌的分类学地位。【方法】通过平板划线分离法分离纯化胡杨树干部内存液样品中的内生酵母菌;检测分离菌株形态学特征和生理生化特征,进一步对酵母菌株的26... 详细信息

【目的】依据形态学、生理生化和26S rDNA D1/D2区序列特征,确定一株胡杨内生酵母菌的分类学地位。【方法】通过平板划线分离法分离纯化胡杨树干部内存液样品中的内生酵母菌;检测分离菌株形态学特征和生理生化特征,进一步对酵母菌株的26S rDNA D1/D2区序列进行扩增以及系统进化分析。【结果】传统的形态学特征和生理生化特征鉴定及26S rDNA D1/D2区序列分析结果表明菌株ZA-7与Rhodotorulaminuta(FR870029)相近,相似率为99.4%,存在4个碱基的差异。【结论】从胡杨树干液中分离得到了一株尚未从植物中分离到的内生酵母菌,并将该菌株鉴定为Rhodotorula minuta的近似种。

关键词：胡杨内生酵母菌 Rhodotorula minuta 26S rDNA D1/D2区序列