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Identification for Heat Tolerance in Backcross Recombinant Lines and Screening of Backcross Introgression Lines with Heat Tolerance at milky stage in Rice

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Rice science 2011年第4期18卷 279-286页

作者： Jiang-lin LIAO Hong-yu ZHANG Xue-lian SHAO Ping-an ZHONG Ying-jin HUANG Key Laboratory of Crop Physiological Ecology and Genetic Breeding Ministry of Education Jiangxi Agricultural University Nanchang 330045 China

The present study aimed at breeding new rice germplasms with similar genome but significantly differed in heat tolerance during the grain filling stage.A total of 791 BC1F8 backcross recombinant lines,derived from the cross of Xieqingzao B /N22 //Xieqingzao B,were used as *** rice line was separated evenly into two groups,and the heat tolerance of all rice lines were evaluated at natural high temperature in *** rice lines with heat tolerant index higher than 90% or lower than 40% were selected to compare the phenotypic characters and further identify heat tolerance at the early milky stage in a *** lines with similar phenotypic characters but significantly differed in heat tolerance at the milky stage were analyzed by 887 simple sequence repeat markers that were evenly distributed on the 12 rice *** the result,12(6 pairs) rice lines with similar phenotypic characters but significantly differed in heat tolerance at the milky stage were *** marker analysis indicated that the genomic polymorphism between 703T and 704S was the smallest in the 6 pairs of rice lines,with only 16 polymorphic sites,including 22 different *** application of these two backcross introgression rice lines for future study on the mechanisms of heat tolerance in rice at the milky stage will be theoretically beneficial in reducing the interference caused by genetic differences from experimental materials.

关键词： rice heat tolerance milky stage backcross recombinant line backcross introgression line

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