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Therapeutic effects of adipose-derived stem cells-based microtissues on erectile dysfunction in streptozotocin-induced diabetic rats

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Asian Journal of Andrology 2017年第1期19卷 91-97页

作者： Feng Zhou Hua Xin Yong-De Xu Hong-En Lei Bi-Cheng Yang Rui-Li Guan Meng Li Jian-Quan Hou Zhong-Cheng Xin Molecular Biology Laboratory of Andrology Center Peking University First Hospital Peking University Beijing 100034 China Department of Urology First Affiliated Hospital of Soochow University Soochow University Suzhou 215006 China Department of Ophthalmology Beijing Chao-Yang Hospital Capital Medical University Beijing 100043 China Department of Urology General Hospital of Ningxia Medical University Ningxia Medical University Ningxia 750021 China

This study aimed to explore the therapeutic effects of adipose-derived stem cells （ADSCs）-based microtissues （MTs） on erectile dysfunction （ED） in streptozotocin （STZ）-induced diabetic rats. Fifty-six 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ （60 mg kg-1）, and 8 weeks later, the determined diabetic rats randomly received intracavernous （IC） injection of phosphate buffer solution （PBS）, ADSCs, or MTs. Another eight normal rats equally got IC injection of PBS. MTs were generated with a hanging drop method, and the injected cells were tracked in ADSC- and MT-injected rats. Four weeks after the treatments, intracavernous pressure （ICP）, histopathological changes in corpus cavernosum （CC）, and functional proteins were measured. Rat cytokine antibody array was used to detect ADSCs or MTs lysate. The results showed that MTs expressed vascular endothelial growth factor （VEGF）, nerve growth factor （NGF）, and tumor necrosis factor-stimulated gene-6 （TSG-6）. MTs injection had a higher retention than ADSCs injection and MTs treatment improved ICP, neuronal nitric oxide synthase （nNOS） expression, smooth muscle, and endothelial contents in diabetic rats, ameliorated local inflammation in CC better. Thus, our findings demonstrate that IC injection of MTs improves erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

关键词： adipose-derived stem cells diabetes erectile dysfunction microtissues paracrine factors

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Encapsulated three-dimensional bioprinted structure seeded with urothelial cells:a new construction technique for tissue-engineered urinary tract patch

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Chinese Medical Journal 2020年第4期133卷 424-434页

作者： Yi-Peng Jin Chong Shi Yuan-Yi Wu Ji-Lei Sun Jiang-Ping Gao Yong Yang Department of Urology Sixth Medical Center of Chinese People's Liberation Army General HospitalBeijing 100048China The First Center of Department of Hepatobiliary Surgery Fifth Medical Center of Chinese People's Liberation Army General HospitalBeijing 100039China Department of Urology Fourth Medical Center of Chinese People's Liberation Army General HospitalBeijing 100048China Department of Urology First Medical Center of Chinese People's Liberation Army General HospitalBeijing 100853China

Background:Traditional tissue engineering methods to fabricate urinary tract patch have some drawbacks such as compromised cell viability and uneven cell distribution within *** this study,we combined three-dimensional(3D)bioprinting and tissue engineering method to form a tissue-engineered urinary tract patch,which could be employed for the application on Beagles urinary tract defect mode to verify its effectiveness on urinary tract ***:Human adipose-derived stem cells(hADSCs)were dropped into smooth muscle differentiation medium to generate induced microtissues(ID-MTs),flow cytometry was utilized to detect the positive percentage for CD44,CD105,CD45,and CD34 of *** of vascular endothelial growth factor A(VEGFA)and tumor necrosis factor-stimulated gene-6(TSG-6)in hADSCs and MTs were identified by Western *** the ID-MTs were employed for 3D *** bioprinted structure was encapsulated by transplantation into the subcutaneous tissue of nude mice for 1 *** retrieval of the encapsulated structure,hematoxylin and eosin and Masson’s trichrome staining were performed to demonstrate the morphology and reveal collagen and smooth muscle fibers,integral optical density(IOD)and area of interest were calculated for further semiquantitative *** double staining of CD31 andα-smooth muscle actin(α-SMA)were used to reveal vascularization of the encapsulated *** was performed to evaluate the expression of interleukin-2(IL-2),α-SMA,and smoothelin of the MTs in the implanted ***,the encapsulated structure was seeded with human urothelial *** staining of cytokeratins AE1/AE3 was applied to inspect the morphology of seeded encapsulated ***:The semi-quantitative assay showed that the relative protein expression of VEGFA was 0.355±0.038 in the hADSCs vs.0.649±0.150 in the MTs(t=3.291,P=0.030),while TSG-6 expression was 0.492±

关键词： Human adipose-derived stem cells Urinary tract patch microtissues Tissue engineering Three-dimensional bioprinting

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